| Project/Area Number |
02558016
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| Research Category |
Grant-in-Aid for Developmental Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
物質生物化学
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| Research Institution | Institute for Protein Research, Osaka University |
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Principal Investigator |
SHIMONISHI Yasutsugu Inst. for Protein Research, Osaka Univ. Professor., たんぱく質研究所, 教授 (00029951)
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| Co-Investigator(Kenkyū-buntansha) |
KAMMEI Yoshihiro MS group, JEOL Ltd., Group leader, 質量分析開発グループ, グループ長
TAKAO Toshifumi Inst. for Protein Research, Osaka Univ. Instructor, たんぱく質研究所, 助手 (10197048)
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| Project Period (FY) |
1990 – 1991
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| Project Status |
Completed (Fiscal Year 1991)
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| Budget Amount *help |
¥7,200,000 (Direct Cost: ¥7,200,000)
Fiscal Year 1991: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 1990: ¥6,200,000 (Direct Cost: ¥6,200,000)
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| Keywords | Four-sector Tandem mass spectrometer / CPU-controlled MS / MS / Acquisition Processor Unit / Digital Analog Converters / Multi-precursor lons / Fragment Ions / Stable-isotope Labeling of peptides / Peptide And Protein Sequencing / 安定同位体 ^<18>O標識化 / タンデム質量分析計 / 自動制御システム / マルチプリカ-サ-イオン捕促システム / マススペクトル表示 / マスクロマトグラム表示 |
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| Research Abstract |
We have developed a new acquisition processor unit simultaneously to control MS1 and MS2 of a four-sector tandem mass spectrometer that makes it possible to acquire the MS/MS spectra from multi-precursor ions in a single measurement. The difficulty in operation of the present four-sector tandem mass spectrometer (MS1 needs to be manually switched mass to mass to the center of each precursor ion, resulting in time and sample loss) can be overcome by connecting it to the above control system.
In the present system, to switch the magnetic field to the precise center of the precursor ions in a MS1, it can be correctly compensated by re-calibration using an appropriate calibrant interpolating each selected mass. Each precursor ion automatically selected in the MS1 can be decomposed independently by bombardment with helium in a collision cell, and resultant fragment ions derived from each precursor ion can be readily analyzed in a MS2. In this process, four sets of digital analo, converters (DAC) controlling magnetic and electric fields of both MSI and MS2 can be syiicliroiiized by system engineering work station (a data acquisition system in JMS-HX221CP, Institute for Protein Research).
A four-sector tandem mass spectrometer (JMS-HX221CP) equipped with the above MS/MS control system was used for analyzing, primary structures of peptides and proteins. The CPUcontrolled MS/MS of multi-precursor ions was found to be effective and rapid in peptide and protein sequencing. F Urthermore, a combined use of a stable-isotope labeling, method of peptides and the present tandem mass spectrometer allowed easily and reliably to determine amino acid sequences of peptides and proteins.
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