• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

The regulatory mechanisms for maintaining the chlorophyll to apoproteins ratio.

Research Project

Project/Area Number 02640520
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field 植物生理学
Research InstitutionKYOTO UNIVERSITY

Principal Investigator

TANAKA Ayumi  Kyoto University,Instructor, 理学部, 助手 (10197402)

Project Period (FY) 1990 – 1992
Project Status Completed (Fiscal Year 1992)
Budget Amount *help
¥1,800,000 (Direct Cost: ¥1,800,000)
Fiscal Year 1992: ¥300,000 (Direct Cost: ¥300,000)
Fiscal Year 1991: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 1990: ¥1,000,000 (Direct Cost: ¥1,000,000)
KeywordsChlorophyll / Chlorophyll-protein complex / Light-harvesting chlorophyll-protein complex / Chloroplast / Protease / クロロフィルタンパク質複合体 / 集光性クロロフイルタンパク質複合体 / クロロフィルータンパク質複合体 / 緑化
Research Abstract

When etiolated seedlings are illuminated,chlorophyll(Chl)begins to be synthesized with accumulation of Chl-protein complex(CP)apoproteins. Newly synthesized Chl is incorporated into apoproteins to form CPs,which are associated with other components to form functional photosystems.
Each CP is supposed to be composed of Chl and its apoprotein in a definite ratio. It have been shown that light-harvesting Chl a/b-protein complex of PSII binds 15 Chl. The Chl/P700 ratio for P700-Chl a-protein complex isolated by SDS-PAGE was reported to be 120. Two apoproteins of PSII core have definite ratios of Chl to apoproteins Taking into consideration the observation that all the Chl molecules bind to apoproteins in thylakoid membranes,these indicate that the ratio of Chl to apoproteins of mature chloroplasts would be kept constant.
Accumulation of excess Chl or apoproteins which do not bind with each other would not be favorable for chloroplasts. The accumulation of apoproteins must be regulated in accordance with changes in the rate of Chl synthesis. We focussed our attention on the regulatory mechanisms operate to maintain the Chl to apoprotein ratio constant during chloroplast development and the followings were elucidated.
(1)Chl is supplied to apoproteins in two ways:by distribution of newly synthesized Chl and by redistribution of Chl that have been previously incorporated into other CPs. (2)The formation of different CPs is regulated by differential affinities of the apoproteins for Chl.(3)Coordinate accumulation of Chl and apoproteins of CPs is achieved through rapid digestion of apoproteins which are not bound with Chl.

Report

(4 results)
  • 1992 Annual Research Report   Final Research Report Summary
  • 1991 Annual Research Report
  • 1990 Annual Research Report
  • Research Products

    (25 results)

All Other

All Publications (25 results)

  • [Publications] 大橋 和子: "Development of thylakoid components in plastids during the early phase of greening in barley leaves." Plant Cell Physiol.33. 371-377 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] 田中 義人: "Stabilization of apoproteins of light-harvesting chlorophyll a/b-protein complexes by feeding 5-aminolevulinic acid under intermittent illumination" Plant Physiol.Biochem.30. 123-128 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] 加藤 哲也: "Xanthomes,supramolecular assemblies of xanthiphyll-chlorophylla/c protein complexes." Methods in Enzymology.(1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] 田中 義人: "Effect of 5-aminolevulinic acid on the accumulation of chlorophyll b and apoproteins of light-harvestion chlorophyll a/b-porotein complex of photosystem II." Plant Cell Physiol.(1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] 和田 明: "A 5-kDa protein (SCS23)from the 30S subunit of the spinach chloroplast ribosome." FEBS Lett.(1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] 柴坂 三根夫: "Changes in spectral properties of chlorophyll during greening of barley leaves." Photochem.Photobiol.(1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Shimada,Y.: "Formation of chlorophyll-protein complexes during greening l.Distribution of newly synthesized chlorophyll." Plant Cell Phsiol.31. 639-647 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Tanaka,A.: "Formation of chlorophyll-protein.Redistribution of chlorophyll amongapoproteins" Plant Cell Physiol.32. 195-204 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Honda,H.: "Proteolytic digestion of apoproteins of light-harvesting chlorophyll a/b-protein complexes in barley leaves." In Regulation of Chloroplast Biogenesis. 337-341 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Ohashi,K.: "Development of thylakoid components in plastids during the early phase of greening in barley leaves." Plant Cell Physiol.33. 371-377 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Tanaka,Y.: "Stabilization of apoproteins of light-harvesting chlorophyll a/b-protein complexes by feeding 5-aminolevulinic acid under intermittent illumination." Plant Physiol.Biochem.30. 123-128 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Katoh,T.: "Xanthomes,supramolecular assemblies of xanthiphyll-chlorophylla/c protein complexes." Methods in Enzymology.(1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Tanaka,Y.: "Effect of 5-aminolevulinic acid on the accumulation of chlorophyll b and apoproteins of light-harvesting chlorophyll a/b-protein complex of photosystem II." Plant Cell Physiol.(1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Wada,A.: "A 5-kDa protein(SCS23)from the 30S subunit of the spinach chloroplast ribosome." FEBS Lett.(1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Shibashaka,M.: "Changes in spectral properties of chlorophyll during greening of barley leaves." Photochem.Photobiol.(1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] 大橋 和子: "Development of thylakoid components in plastids during the early phase of greening barley leaves." Plant Cell Physiol.33. 371-377 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] 田中 義人: "Stavilization of apoproteins of light-Harvesting chlorophyll a/b-protein complexes by feeding 5-aminolevulinic acid under intermittent illumination." Plant Physiol.Biochem.30. 123-128 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] 加藤 哲也: "Xanthomes,supramolecular assemblies of xanthiphyll-chlorophylla/c protein complexes." Methods in Enzymology.(1993)

    • Related Report
      1992 Annual Research Report
  • [Publications] 田中 義人: "Effect of 5-aminolevulinic acid on the accumulation of chlorophyll b and apoproteins of light-harvesting chlorophyll a/b-protein complex of photosystem II." Plant Cell Physiol.(1993)

    • Related Report
      1992 Annual Research Report
  • [Publications] 和田 明: "A 5-KDa protein(SCS23)from the 30S subunit of the spinach chloroplast ribosome." FEBS Lett.(1993)

    • Related Report
      1992 Annual Research Report
  • [Publications] 田中 歩: "Formation of chlorophyllーprotein complexes drgduring greening.2Redistribution of chlorophyll among apoproteins." Plant Cell Physiol.32. 195-204 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] 本多 忠紀: "Proteolytic digestion of apoproteins of lightーharesfn harvesting chlorophyll a/bーprotein complexes in barley leaves." Regulation of chloroplast biogenesis. 329-334 (1992)

    • Related Report
      1991 Annual Research Report
  • [Publications] 田中 義人: "Stabilization of apoproteins of lightーharvesting chlorophyll a/bーprotein complex by feeding 5ーaminolevulinic acid under intermittent illumination." Plant Physiol.Biochem.30.

    • Related Report
      1991 Annual Research Report
  • [Publications] 島田 幸久: "Formation of chlorophyllーprotein complexes during greening 1.Distribntion of newly synthesized chlorophyll among apoproteins" Plant Cell Physiol.31. 639-647 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] 田中 歩: "Formation of chlorophyllーprotein complexes during greening 2.Redistribation of chlorophyll among apoproteing" Plant Cell Physiol.32. (1991)

    • Related Report
      1990 Annual Research Report

URL: 

Published: 1990-04-01   Modified: 2016-04-21  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi