Analytical studies on fern anthridium differentiation induced by anteridiogen
| Project/Area Number |
02640537
|
|---|
| Research Category |
Grant-in-Aid for General Scientific Research (C)
|
| Allocation Type | Single-year Grants |
|---|
| Research Field |
植物形態・分類学
|
|---|
| Research Institution | Toyama University |
|---|
Principal Investigator |
SUGAI Michizo Toyama Univ., Fac. of Science, Professor, 理学部, 教授 (60109098)
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
MASUDA Kyojiro Toyama Univ., Fac. of Science, Instructor, 理学部, 助手 (90018987)
YAMADA Kyoji Toyama Univ., Fac. of Science, Assoc. Professor, 理学部, 助教授 (70200714)
|
|---|
| Project Period (FY) |
1990 – 1991
|
| Project Status |
Completed (Fiscal Year 1991)
|
| Budget Amount *help |
¥1,500,000 (Direct Cost: ¥1,500,000)
Fiscal Year 1991: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 1990: ¥1,000,000 (Direct Cost: ¥1,000,000)
|
|---|
| Keywords | Antheridiogen / Antheridium / Lygodium japonicum (fern) / Fern Gametophyte / Gibberellic Acids / Protein / Protoplasts / Synchronous Culture / 造精器分化 / 突然変異体 / 細胞分化 / 遺伝子発現 |
|---|
| Research Abstract |
1) Establishment of experimental system of synchronous induction of antheridia
Antheridia were induced synchronously in darkness on protonemata of Lygodium japonicum cultured in agar media containing GA_3 of GA_4 methyl ester. Antheridia were also induced within 10 days by red light irradiated for 24 hs in liquid media containing 10^<-5> M GA_3.
Protoplasts isolated from gametophytic tissue in Lygodium Japonicum were cultured under the condition of radual decresing the osmotic pressuree of the media. In this culture conditions antheridia were induced on regenerated gametophytes within 3 days after onset of GA_3 treatment.
2) Detection of protein by SDS-PAGE
Soluble protein fraction extracted from protonemata grown in GA_3 containing liquid media were analyzed by SDS-PAGE. Protein bands were newly detected at about MW. 38, 000 and 69, 000 in antherdium bearing protonema. The appearance of the new protein bands were closely associated with the induction of the antheridia.
|
Report
(3 results)
Research Products
(16 results)
