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Molecular and morphological analyses of maternal inheritance of Chlamydomonas mutants by fluorescence microscopy

Research Project

Project/Area Number 02640542
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field 植物形態・分類学
Research InstitutionUniversity of the Ryukyus

Principal Investigator

NAKAMURA Soichi  University of the Ryukyus Division of General Education associate professor, 教養部, 助教授 (00201674)

Project Period (FY) 1990 – 1992
Project Status Completed (Fiscal Year 1992)
Budget Amount *help
¥1,800,000 (Direct Cost: ¥1,800,000)
Fiscal Year 1992: ¥300,000 (Direct Cost: ¥300,000)
Fiscal Year 1991: ¥300,000 (Direct Cost: ¥300,000)
Fiscal Year 1990: ¥1,200,000 (Direct Cost: ¥1,200,000)
KeywordsMaternal inheritance / Plastid / Pt nucleoids / Fluorescence microscope / Mutant / Preferential digestion / DNA amount / Number of pt nucleoids / 精細胞 / ヌクレア-ゼC / 葉緑体核 / クラミドモナス
Research Abstract

Maternal inheritance is a phenomenon that the characteristics of plastids of only maternal parent are transmitted to all progenies. Therefore we attempted to isolate mutants which have morphologically abnormal characteristics of plastid or plastid nucleoids (pt nucleoids). Two mutant cells (m-1, 2), having about 20 and 50 of plastid nucleoids, respectively, were isolated. Their numbers of pt nucleoids were about two and five times that of wild type cells, respectively. From the physiological analysis, total DNA contents of pt nucleoids per plastid were two and five times that of a wild type cell.
Preferential digestion of pt nucleoids derived from a male parent in newly formed young zygotes is thought to be the primary cause of the phenomenon of maternal inheritance of plastids. Usually the frequency of preferential digestion is 100% in the zygotes at 5 h. after crossing between two parents of wild type cells. However, the frequency was 85% in the zygotes when a m-1 cell was used as fem … More ale parent to cross to a male parent of wild type cell. In the zygotes when a m-2 cell was used as the female parent, the frequency was 80%.
Genetic analysis showed that each of two mutants had one gene mutation. Two mutations isolated in the present experiment were probably linked because no tetra type tetrads were obtained among 31 tetrads after crossing between two mutant cells.
Contrarily, two mutant cells (m-3, 4), having small pt nucleoid number, were isolated. Genetic analysis showed that they had one gene mutation. The results obtained from m-1 and m-2 mutant cells probably indicate that the preferential digestion of pt nucleoids in zygotes derived from these mutants requires a longer period because the frequencies of preferential digestion were not low. This suggests that maternal inheritance occurs independently on the DNA amount of pt nucleoids and opposes a proposal that one of parents having more DNA amount of pt nucleoids can transmit to its plastid characteristics to all progenies. Less

Report

(4 results)
  • 1992 Annual Research Report   Final Research Report Summary
  • 1991 Annual Research Report
  • 1990 Annual Research Report
  • Research Products

    (24 results)

All Other

All Publications (24 results)

  • [Publications] Sodmergen et al.: "Behavior of organelle nuclei(nucleoids)in generative and vegetative cells during maturation of pollen in Lilium longiflorum and Pelargonium zonale." Protoplasma. 168. 73-82 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Uchida H.et al.: "A study on the effects of UV on preferential digestion of chloroplast nuclei in young zygote of Chlamydomonas reinhardtii." Cytologia. 57. 395-399 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Nakamura S.et al.: "Fluorescence microscopy of plastid nucleoids and a survey of nuclease C in higher plants with respect to mode of plastid inheritance." protoplasma. 169. 68-74 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] 中村 宗一、その他: "シロツメクサからのヌクレアーゼCの分離" 日本植物学会第57回大会記録. 261- (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] 崎原 美奈子、その他: "クラミドモナスにおける葉緑体の核様体数の異常に多くなった株の単離" 日本植物学会第57回大会記録. 261- (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] 内田 英伸、その他: "Chlamydomonas reinhardtiiの接合直後に発現される遺伝子群の解析" 日本植物学会第57回大会記録. 264- (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Nakamura S, Chibana H & Kuroiwa T: "Domination by female cells over preferential digestion of chloroplast nucleoids in Chlamydomonas reinhardtii." Plant Cell Physiol. 32. 359-364 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Sodmergen, Suzuki T, Kawano S, Nakamura S, Tano S & Kuroiwa T.: "Behavior of organelle nuclei (nucleoids) in generative and vegetative cells during maturation of pollen in Lilium longiflorum and Pelargonium zonal." Protoplasma. 168. 73-82 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Uchida H, Kawano S, Nakamura S & Kuroiwa T.: "A study on the effects of UV on preferential digestion of chloroplast nuclei in young zygote of Chlamydomonas reinhardtii." Cytology. 57. 395-399 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Nakamura s, Ikehara T, Uchida H, Suzuki T & Sodmergen: "Fluorescence microscopy of plastid nucleoids and a survey of nuclease C in higher plants with respect to mode of plastid inheritance." Protoplasma. 169. 68-74 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Sodmergen et al.: "Behavior of organelle nuclei (nucleoids)in generative and vegetative cells during maturation of pollen in Lilium longiflorum and pelargonium zonale." Protoplasma. 168. 73-82 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] Uchida H. et al.: "A study on the effects of UV on preferential digestion of chloroplast nuclei in young zygote of Chlamydomonas reinhardtii." Cytologia. 57. 395-399 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] Nakamura S.et al.: "Fluorescence microscopy of plastid nucleoids and a survey of nuclease C in higher plants with respect to mode of plastid inheritance." protoplasma. 169. 68-74 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] 中村 宗一,その他: "シロツメクサからのヌクレアーゼCの分離" 日本植物学会第57回大会記録. 261- (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] 崎原 美奈子,その他: "クラミドモナスにおける葉緑体の核様体数の異常に多くなった株の単離" 日本植物学会第57回大会記録. 261- (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] 内田 英伸、その他: "Chlamydomonas reinhardtiiの接合直後に発現される遺伝子群の解析" 日本植物学会第57回大会記録. 264- (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] S.Nakamura: "Domination by female cells over preferential digestion of chloroplast nucleoids in Chlamydomonas reinhardtii." Plant Cell physiology. 32. 359-364 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] S.Nakamura: "Fluorescence microscopy of plastid nucleoids and a survey of nuclease C in higher plants with respect to mode of plastid inheritance." Protoplasma.

    • Related Report
      1991 Annual Research Report
  • [Publications] 中村 宗一: "クラシドモナスにおける葉緑体核様体の形態の異常な株の単離" 日本植物学会第56回大会研究発表記録. 166 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] 蘇都莫日根: "母性遺伝型植物と両性遺伝型植物の花粉形成時における成殖細胞オルガネラDNAの挙動に関する分子細胞生物学的解析" 日本植物学会第56回大会研究発表記録. 169 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] 内田 英伸: "クラミドモナス(Chlamydomonas reinhardtii)で接合直後に発現される遺伝子のcDNAクロ-ニング" 日本植物学会第56回大会研究発表記録. 286 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] S.Nakamura: "Domination by female cellsover preferential digestion of chloroplast nucleoids in Chlamydomonasreinhardtii." Plant Cell Physiol.

    • Related Report
      1990 Annual Research Report
  • [Publications] 中村 宗一: "クラミドモナスにおける配偶子の加齢による葉緑体の形質の母遺伝への影響" 日本植物学会第55回大会発表記録. 162 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] 内田 英伸: "クラミドモナス母性遺伝作用機構への核からの情報の関与" 日本植物学会第55回大会発表記録. 245 (1990)

    • Related Report
      1990 Annual Research Report

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Published: 1990-04-01   Modified: 2016-04-21  

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