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Analysis of flagellar movement of golden hamster spermatozoa by measuring sliding between outer doublet microtubules

Research Project

Project/Area Number 02640551
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field 動物発生・生理学
Research InstitutionTokyo Institute of Technology

Principal Investigator

ISHIJIMA Sumio  Tokyo Inst. Tech. Fac. of Sci. Associate, 理学部, 助手 (70193315)

Project Period (FY) 1990 – 1991
Project Status Completed (Fiscal Year 1991)
Budget Amount *help
¥1,500,000 (Direct Cost: ¥1,500,000)
Fiscal Year 1991: ¥300,000 (Direct Cost: ¥300,000)
Fiscal Year 1990: ¥1,200,000 (Direct Cost: ¥1,200,000)
KeywordsFlagellar movement / Hamster / Microtubule / Sliding / Sperm
Research Abstract

1. The method of "flagellar model", which is demembranated spermatozoa showing the flagellar movement similar to that of intact spermatozoa when activated with MgATP^<2->, has been developed in golden hamster spermatozoa. The direction of rolling motion of a spermatozoon about its long axis and of yawing motion of spermatozoa under the coverslip as well as the amplitude and asymmetry of flagellar wave was changed by the Ca^<2+> concentration in reactivation solution. In contrast to the sea urchin spermatozoa, the majority of intact golden hamster spermatozoa rolled counterclockwise when viewed from the head. Thus, intracellular concentration of Ca^<2+> of golden hamster spermatozoa is perhaps different from that of sea urchin spermatozoa.
2. When the demembranated spermatozoa were treated with DTT and trypsin, and then MgATP^<2-> was added to them, sliding of the outer doublet microtubules from the axoneme was observed under a darkfield microscope. The velocity of this sliding, which was determined by measuring the length of microtubules and time, was approximately 8 um/s. The sliding velocity did not change by the Ca^<2+> concentration in the medium so much.
3. There was no significant difference in the sliding velocity among testicular and caput and cauda epididymal spermatozoa. These results confirmed that the motor apparatus in these spermatozoa is already functionally assembled in the testis, although these spermatozoa are immotile in the Tyrode's solution.

Report

(3 results)
  • 1991 Annual Research Report   Final Research Report Summary
  • 1990 Annual Research Report
  • Research Products

    (29 results)

All Other

All Publications (29 results)

  • [Publications] Ishijima,S.: "Rotational movement of a spermatozoon around its long axis" Journal of Experimental Biology. 163. 15-31 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] 石島 純夫: "精子の運動における流体力学の問題" 日本機械学会第2回バイオエンジニアリングシンポジウム講演論文集. 99-100 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Ishijima,S.: "Demembranation and reactivation of mammalian spermatozoa from golden hamster and ram" Methods in Enzymology. 196. 417-428 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Hamaguchi,M.S.: "Double-focal videomicroscopy:A simple video system for analyzing the dynamics of cell motility" Journal of Experimental Biology. 161. 537-541 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] 石島 純夫: "哺乳類精子の運動能獲得と受精能獲得における鞭毛運動の変化" 日本アンドロロジ-学会第9回学術大会講演抄録集. 28-30 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Gagnon,C.: "Controls of Sperm Motility" CRC Press, 395 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Bavister,B.D.: "Fertilization in Mammals" Serono Publications, 469 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] 毛利 秀雄: "精子学" 東大出版会,

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Baccetti,B.: "Comparative Spermatology" Raven Press,

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Ishijima, S.: "Rotational movement of a spermatozoon around its long axis" J. Exp. Biol.163. 15-31 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Ishijima, S.: "Hydrodynamic aspects of sperm motility" Proceedings of the 2nd Symposium on Bioengineering. 99-100 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Ishijima, S.: "Demembranation and reactivation of mammalian spermatozoa from golden hamster and ram" Method. Enzymology. 196. 417-428 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Hamaguchi, M. S.: "Double-focal videomicroscopy : A simple video system for analyzing the dynamics of cell motility" J. Exp. Biol.161. 537-541 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Ishijima, S.: "Changes in the flagellar movement of mammalian spermatozoa during maturation and capacitation" Proceedings of the 9th Annual Meeting of the Japanese Society of Andrology. 28-30 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Gagon, C.: CRC Press. Controls of Sperm Motility, (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Bavister, B. D.: Serono Publications. Fertilization in Mammals, (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Mohri, H.: University of Tokyo Press. Spermatology,

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Baccetti, B.: Raven Press. Comparative Spermatology,

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Ishijima,S.: "Rotational movement of a spermatozoon around its long axis" Journal of Experimental Biology. 163. 15-31 (1992)

    • Related Report
      1991 Annual Research Report
  • [Publications] 石島 純夫: "精子の運動における流体力学の問題" 日本機械学会第2回バイオエンジニアリングシンポジウム講演論文集. 99-100 (1992)

    • Related Report
      1991 Annual Research Report
  • [Publications] Ishijima,S.: "Demembranation and reactivation of mammalian spermatozoa from golden hamster and ram" Methods in Enzymology. 196. 417-428 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] Hamaguchi,M.S.: "Doubleーfocal videomicroscopy:A simple video system for analyzing the dynamics of cell motility" Journal of Experimental Biology. 161. 537-541 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] 毛利 秀雄: "精子学" 東大出版会,

    • Related Report
      1991 Annual Research Report
  • [Publications] Ishijima,S.: "Rotational movement of a spermatozoon around its long axis" Journal of Experimental Biology.

    • Related Report
      1990 Annual Research Report
  • [Publications] Hamaguchi,M.S.: "Doubleーfocal videomicroscopy:A simple video system for analyzing the dynamics of cell motility" Journal of Experimental Biology.

    • Related Report
      1990 Annual Research Report
  • [Publications] Ishijima,S.: "Preparation of demembranated and reactivated golden hamster and ram spermatozoa" Methods in Enzymology.

    • Related Report
      1990 Annual Research Report
  • [Publications] Gagnon,C.: "Controls of Sperm Motility" CRC Press, 395 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] Bavister,B.D.: "Fertilization in Mammals" Serono Symposia,USA, 469 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] 毛利 秀雄: "精子学" 東大出版会,

    • Related Report
      1990 Annual Research Report

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Published: 1990-04-01   Modified: 2016-04-21  

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