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Structural analysis of the hemoglobin genes in Paramecium

Research Project

Project/Area Number 02640553
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field 動物発生・生理学
Research InstitutionNiigata University

Principal Investigator

USUKI Itaru  College of General Education, Niigata University, Professor, 教養部, 教授 (90018242)

Co-Investigator(Kenkyū-buntansha) OCHIAI Takehiko  Fukushima Medical College, Professor, 教授 (40075031)
YAMAUCHI Kiyoshi  Faculty of Science, Shizuoka University, Research Assistant, 理学部, 助手 (50201827)
Project Period (FY) 1990 – 1991
Project Status Completed (Fiscal Year 1991)
Budget Amount *help
¥1,400,000 (Direct Cost: ¥1,400,000)
Fiscal Year 1991: ¥600,000 (Direct Cost: ¥600,000)
Fiscal Year 1990: ¥800,000 (Direct Cost: ¥800,000)
KeywordsParamecium / hemoglobin / genome DNA / cDNA / primary structure / nucleotide sequence / intron / amino acid residue
Research Abstract

This project was started to make clear the mutual correlation among the heterogeneous hemoglobin components and the structure of the hemoglobin genes in Paramecium.
We found that, using 6 stocks in 3 different species of Paramecium, 1) relative amounts of hemoglobin components shows a definite change during the cell growth and by the temperature of the culture, 2) the changes occur between the major hemoglobin component and submajor one, 3) the former in a cell shows faster migration by PAGE, smaller in molecular weight and lower in pI than the latter, and 4) both of the components increase or decrease inversely their amounts each other.
It was confirmed that the major hemoglobin components (Hb10) from 15 stocks in five sibling species of P. caudatum are dividable into three types, as A, B, and A + B, although their pI values fall into a narrow range measuring about pH 3.9. The submajor hemoglobin component in this species is also divided into four variants, showing the pI value of pH 9.8-10.5.
We determined the nucleotide sequence of the cDNA encoding the A-type of Hb10. The results obtained indicate that the sequence contains four TAA codons, which are known to act as a termination signal in the most of eukaryotes but encode Gln for ciliates, and that the Hb has an unusual structure which is composed of 116 amino acid residues. Analysis of the genomic DNA from macronuclei made clear that the gene is organized with two exons interrupted by one small intron, which is composed of only 23 bp at the position corresponding to the second intron found in plant Hb genes. We also clarified that the gene encoding the B-type of Hb10 are identical to that of the A-type Hb10, in an exception of the replacements of five out of 116 amino acid residues.

Report

(3 results)
  • 1991 Annual Research Report   Final Research Report Summary
  • 1990 Annual Research Report
  • Research Products

    (23 results)

All Other

All Publications (23 results)

  • [Publications] Usuki,I.and Hino,A.: "Changes in relative concentrations of the hemoglobin components in Paramecium caused by cell growth and temperature of the culture." Journal of Cell Science. 100(3). 635-639 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Hirai,I.,Usuki,I.and Ochiai,T.: "Inter- and intrasyngenic variations of the hemoglobin components in Paramecium caudatum."

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Yamauchi,K.,Mukai,M.,Ochiai,T.and Usuki,I.: "Molecular cloning of the cDNA for the major hemoglobin component from Paramecium caudatum." Biochemical and Biophysical Research Communications. 182(1). 195-200 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Yamauchi,K.,Ochiai,T.and Usuki,I.: "A unique structure of the Paramecium caudatum hemoglobin gene:the presence of one intron corresponding to the second intron found in plant hemoglobin genes."

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Yamauchi,K.,Tada,H.,Ochiai,T.and Usuki,I.: "The presence of subtype of hemoglobin gene in Paramecium caudatum."

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Hirai,I.,Ochiai,T.and Usuki,I.: "Inter-syngenic and inter-stock variations of the acidic and basic hemoglobin components from Paramecium caudatum." Zoological Science. 7(6). 1064 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Mukai,M.,Yamauchi,K.,Ochiai,T.and Usuki,I.: "Colning and sequencing of a cDNA encoding the major hemoglobin component of Paramecium caudatum." Zoological Science. 7(6). 1078 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Yamauchi,K.,Ochiai,T.and Usuki,I.: "Sequencing of complementary DNA encoding the majoa hemoglobin component of Paramecium caudatum." Comparative Physiology and Biochemistry. 8(3-4). 187 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Usuki, I. and Hino, A.: "Changes in relative concentrations of the hemoglobin components in Paramecium caused by cell growth and temperature of the culture." Journal of Cell Science. 100(3). 635-639 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Hirai, I., Usuki, I. and Ochiai, T.: "Inter- and intrasyngenic variations of the hemoglobin components in Paramecium caudatum."

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Yamauchi, K., Mukai, M., Ochiai, T. and Usuki, I.: "Molecular cloning of the cDNA for the major hemoglobin component from Paramecium caudatum." Biochem. Biophys. Res. Commun.182(1). 195-200 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Yamauchi, K., Ochiai, T. and Usuki, I.: "A unique structure of the Paramecium caudatum hemoglobin gene : the presence of one intron corresponding to the second intron found in plant hemoglobin genes."

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Yamauchi, K., Tada, H., Ochiai, T. and Usuki, I.: "The presence of subtype of hemoglobin gene in Paramecium caudatum."

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Hirai, I., Ochiai, T. and Usuki, I.: "Inter-syngenic and inter-stock variations of the acidic and basic hemoglobin components from Paramecium caudatum." Zoological Science. 7(6). 1064 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Mukai, M., Yamauchi, K., Ochiai, T. and Usuki, I.: "Colning and sequencing of a cDNA encoding the major hemoglobin component of Paramecium caudatum." Zoological science. 7(6). 1078 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Yamauchi, K., Ochiai, T. and Usuki, I.: "Sequencing of complementary DNA encoding the major hemoglobin component of Paramecium caudatum." Comp. Physiol. Biochem.8(3-4). 187 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Usuki,I.and Hino,A.: "Chomges in relative concentrations of the hemoglobin components in Paramecium caused by cell growth and temperature of the culture." Journal of Cell Science. 100(Nov.). 635-639 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] Yamauchi,K.,Ochiai,T.and Usuki,I.: "Nucleotide sequence of the cDNA for the major hemoglobin component from Paramecium caudatum." Biochemica Biophysica Research Commumication. (1992)

    • Related Report
      1991 Annual Research Report
  • [Publications] Hirai,I.,Usuki,I.and Ochiai,T.: "Interーand intrasyngenic variations of the hemoglobin components in Paramecium caudatum."

    • Related Report
      1991 Annual Research Report
  • [Publications] Yamauchi,K.,Ochiai,T.and Usuki,I.: "A unique structure of the Paramecium caudatum hemoglobin gene:the presence of one intron corresponding to the second intron found in plant hemoglobin genes."

    • Related Report
      1991 Annual Research Report
  • [Publications] Mukai,M.,Yamauchi,K.,Ochiai,T.and Usuki,I.: "Cloning and sequencing of a cDNA encoding the mojor hemoglobin component of Paramecium caudatum(Abstract)." Zoological Science. 7(6). 1078 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] Hirai,I.,Ochiai,T.and Usuki,I.: "Interーsyngenic and interーstock variations of the acidic and basic hemoglobin components from Paramecium caudatum(Abstract)." Zoological Science. 7(6). 1064 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] Yamauchi,K.,Ochiai,T.and Usuki,I.: "Nucleotide sequence of the cDNA for the major hemoglobin component from Paramecium caudatum." Journal of Molecular Biology.

    • Related Report
      1990 Annual Research Report

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Published: 1990-04-01   Modified: 2016-04-21  

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