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Mechanism of Signal Transduction in the Chromatophore Membrane

Research Project

Project/Area Number 02640570
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field 動物発生・生理学
Research InstitutionToho University

Principal Investigator

FUJII Ryozo  Toho University, Faculty of Science, Professor, 理学部, 教授 (10045354)

Co-Investigator(Kenkyū-buntansha) SUGIMOTO Masazumi  Toho University, Faculty of Science, Research Assistant, 理学部, 助手 (20235899)
OSHIMA Noriko  Toho University, Faculty of Science, Associate Professor, 理学部, 助教授 (70057735)
Project Period (FY) 1990 – 1991
Project Status Completed (Fiscal Year 1991)
Budget Amount *help
¥1,800,000 (Direct Cost: ¥1,800,000)
Fiscal Year 1991: ¥600,000 (Direct Cost: ¥600,000)
Fiscal Year 1990: ¥1,200,000 (Direct Cost: ¥1,200,000)
KeywordsChromatophore / Melanophore / Iridophore / Fish / Signal transduction / Inositol trisphosphate / Calcium ion / Receptor / 色素細胞 / 反射小板
Research Abstract

The concept that membranous phosphatidyl-inositol 1, 4, 5-bisphosphate (PIP_2) is hydrolyzed by phospholipase C to produce inositol 1, 4, 5-trisphosphate (IP_3), which in turn acts on IP_3 receptors on the intracellular Ca^<2+> storage compartment to release the ions has been demonstrated in a few effector cells. In this profect, the sequence has firstly been shown in the chromatophore system. The apparatus which enables us to measure the chromatophore responses and the intracellular Ca^<2+> concentration at the same time was invented by remodelling a commercial industrial microscope system. By applying this to a tilapia melanophore in culture, a quick Ca^<2+> increase followed by melanosome aggregation was demonstrated in response to norepinephrine stimulation. The present results showing the involvement of the IP_3 system strongly suggest that alpha_1-adrenergic receptors are involved in the pigment aggregation in the chromatophores. In practice, both alpha_1 and alpha_2 systems may be operating in fish chromatophores, and the ratios of their incidences may vary among species and also among tissues in a particular species. It is also assumed that alpha_2 system which involves cyclic AMP takes part in relatively slow movement of pigment, whereas alpha_1 system with the IP_3 changes has role in more rapid component of the response. In silurid catfishes, the pigment aggregation in response to sympathetic stimulation is mediated by acetylcholine receptors of the muscarinic type. Working on the translucent glass catfish, we characterized the receptors to be of the M3 type. Significant progresses have also been made withthe studies on motile iridophores. Among them, is a novel aspect on the change of reflective color from dendritic iridophores of the dark sleeper goby.

Report

(3 results)
  • 1991 Annual Research Report   Final Research Report Summary
  • 1990 Annual Research Report
  • Research Products

    (41 results)

All Other

All Publications (41 results)

  • [Publications] Nagaishi,H.: "Light-reflecting properties of the iridophores of the neon tetra Paracheirodon innesi." Comp.BViochem.Phyiol.95A. 337-341 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Oshima,N.: "Evidence for involvement of dynein-tubulin system in pigment aggregation within tilapia melanophores." Comp.Biochem.Physiol.96A. 517-523 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Oshima,N.: "The involvement of calmodulin in motile activities of fish chromatophores." Comp.Biochem.Physiol.97C. 33-36 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Fujii,R.: "Inositol 1,4,5-trisphosphate signals the motile response of fish chromatophores---I.Aggregation ofpigment in the tilapia melanophore." J.Exp.Zool.259. 9-17 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Fujii,R.: "Analysis of the reflection of light from motile iridophores of the dark sleeper,Odontobutis obscura obscura." Zool.Sci.8. 461-470 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] 藤井 良三: "色素細胞1991ー魚類の色素細胞を中心に." 日本香粧品科学会誌. 15. 138-148 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] 藤井 良三: "特集“動物の色科学":序論ー特集によせて." 細胞. 23. 494-495 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] 藤井 良三: "特集“動物の色科学"運動性虹色素胞." 細胞. 23. 507-512 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Fujii,R.: "Blanching at night of denervated banda in teleostean tail fin is due to pigment aggregation in melanophores by melatonin." Comp.Biochem.Physiol.101A. 29-32 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Oshima,N.: "Effects of choline chloride on the pigment translocation within fish melanophores." Comp.Biochem.Physiol.Series C. (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Nagaishi,H.: "Ultrastructure of the motile iridophores of the neon tetra." Zool.Sci.9. (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Oshima,N.: "Study of the motile mechanism in neon tetra iridophores." Comp.Biochem.Physiol.Series A. (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Fujii,R.: "Cytophysiology of fish chromatophores. International Review of Cytology" Academic Press,New York, (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Nagaishi, H.: "Light-reflecting properties of the iridophores of the neon tetra Paracheirodon innesi." Comp. Biochem. Physiol.95A. 937-341 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Oshima, N.: "Evidence for involvement of dynein-tubulin system in pigment aggregation within tilapia melanophores" Comp. Bichem. Physiol.96A. 517-523 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Oshima, N.: "The involvement of calmodulin in motile activities of fish chromatophores." Comp. Biochem. Physiol.96C. 33-36 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Fujii, R.: "Inositol 1, 4, 5-trisphosphate signals the motile response of fish chromatophores---I. Aggregation of pigment in tilapia melanophore" J. Exp. Zool.259. 9-17 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Fujii, R.: "Analysis of the reflection of light from motile iridophores of the dark sleeper, Odontobutis obscura obscura" Zool. Sci.8. 461-470 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Fujii, R.: "Pigment Cell 1991 --- Mainly about fish chromatophores" J. Jpn. Cosmetic Sci. Soc.15. 138-148 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Fujii, R.: "About the special issue : Coloration science in animals" The Cell (Tokyo). 23. 494-495 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Fujii, R.: "Motile iridophores" The Cell (Tokyo). 23. 507-512 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Fujii, R.: "Blanching at night of denervated bands in teleostean tail fin is due to pigment aggregation in melanophores by melatonin" Comp. Biochem. Physiol. 101A. 29-32 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Oshima, N.: "Effects of choline chloride on the pigment translocation within fish melanophores" Comp. Biochem. Physiol. Series C. (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Nagaishi, H.: "Ultrastructure of the motile iridophores of the neon tetra" Zool. Sci.9. (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Oshima, N.: "Study of the motile mechanism in neon tetra iridophores" Comp. Biochem. Physiol., Series A. (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Fujii, R.: Cytophysiology of fish chromatophores. Academic Press, New York, Int. Rev. Cytol., (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] 藤井 良三: "特集“動物の色科学":序論ー特集によせて." 細胞. 23. 494-495 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] 藤井 良三: "特集“動物の色科学":運動性虹色素胞." 細胞. 23. 507-512 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] Fujii,R.: "Blanching at night of denervated bands in teleostean tail fin is due to pigment aggregation in melanophores by melatonin." Comp.Biochem.Physiol.101A. 29-32 (1992)

    • Related Report
      1991 Annual Research Report
  • [Publications] Oshima,N.: "Effects of choline chloride on the pigment translocation within fish melanophores." Comp.Biochem.Physiol.Series c. (1992)

    • Related Report
      1991 Annual Research Report
  • [Publications] Nagaishi,H.: "Ultrastructure of the motile iridophores of the neon tetra." Zool.Sci.9. (1992)

    • Related Report
      1991 Annual Research Report
  • [Publications] Oshima,N.: "Study of the motile mechanism in neon tetra iridophores." Comp.Biochem.Physiol.Series A. (1992)

    • Related Report
      1991 Annual Research Report
  • [Publications] Fujii,R.: "Cytophysiology of fish chromatophores.International Review of Cytology" Academic Press,New York, (1992)

    • Related Report
      1991 Annual Research Report
  • [Publications] Aihara,M.,Kasukawa,H.,Fujii,R.,and Oshima,N.: "Chemical characterization of reflecting platelets in motile iridophores of blue damselfish." Comp.Biochem.physiol.92B. 533-536 (1989)

    • Related Report
      1990 Annual Research Report
  • [Publications] Fujii,R.,Kasukawa,H.,Miyaji,K.,and Oshima,N.: "Mechanism of skin coloration and its changes blueーgreen damselfish,Chromis viridis." Zool.Sci.6. 477-486 (1989)

    • Related Report
      1990 Annual Research Report
  • [Publications] Nagaishi,H.,Nishi,H.,Fujii,R.,and Oshima,N.: "Correlation between body color and behavior in the upsideーdown catfish,Synodontis nigriventris." Comp.Biochem.Physiol.92A. 323-326 (1989)

    • Related Report
      1990 Annual Research Report
  • [Publications] Oshima,N.Kasukawa,H.and Fujii,R.: "Control of chromatophore movements in blueーgreen damselfish,Chromis viridis." Comp.Biochem.Physiol. 93C. 239-245 (1989)

    • Related Report
      1990 Annual Research Report
  • [Publications] Nagaishi,H.Oshima,N.and Fujii,R.: "Lightーrefrecting properties of the iridophores of the neon tetra,Paracheirodon innesi." Comp.Biochem.Physiol.95A. 337-341 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] Fujii,R.,Wakatabi,H.and Oshima,N.: "Inositol 1,4,5ーtrisphosphate signals the motile response of fish chromatophoresーーI.Aggregation of pigment in the tilapia melanophore." J.Exp.Zool.(1991)

    • Related Report
      1990 Annual Research Report
  • [Publications] Fujii,R.,Hayashi,H.,Toyohara,J.and Nishi,H.: "Analysis of the reflection of light from motile iridophores of the dark sleeper,Odontobutis obscura obscura." Zool.Sci.(1991)

    • Related Report
      1990 Annual Research Report
  • [Publications] 藤井 良三: "東大出版会,東京." 第12章 色素細胞と体色変化. メダカの生物学(江上信雄他編),185ー199頁, 315 (1990)

    • Related Report
      1990 Annual Research Report

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Published: 1990-04-01   Modified: 2016-04-21  

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