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Studies on the Isolation and Biological Functions of Amphibian Hypothalamic Hormones

Research Project

Project/Area Number 02640577
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field 動物形態・分類学
Research InstitutionGunma University

Principal Investigator

HAYASHI Hiroaki  Dept. Prot. Chem., Inst. of Endocrinol., Gunma Univ. Assoc. Prof., 内分泌研究所, 助教授 (50008611)

Co-Investigator(Kenkyū-buntansha) TANAKA Sigeyasu  Dept. Morpholgy, Inst. of Endocrinol., Gunma Univ. Res. Assoc., 内分泌研究所, 助手 (90146233)
Project Period (FY) 1990 – 1991
Project Status Completed (Fiscal Year 1991)
Budget Amount *help
¥1,900,000 (Direct Cost: ¥1,900,000)
Fiscal Year 1991: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 1990: ¥1,200,000 (Direct Cost: ¥1,200,000)
KeywordsBullfrog / Hypothalamic hormone / Gonadotropin / Arg-vasotocin / Hydrin / Vasotocin related peptide / ACTH / MSH / アルギニン・バソトシン
Research Abstract

In the amphibia, it is still remain unknown for the nature of the hypothalamic hormones which stimulate the release of the various hormones from the pituitary gland. To identify those hypothalamic hormones, it is important to determine the amount of the pituitary hormones in qualitatively and quantitatively in nanogram level. We have already isolated the two glycoprotein hormones, lutropin (LH) and follitropin (FSH), and determined the complete amino acid sequences of both hormones. Very recently, we also isolated the third glycoprotein hormone, thyrotropin (TSH) from the bullfrog, Rana catesbeiana. We have established the radioimmunoassay systems for the common alpha-subunit for LH, FSH and TSH, and specific beta-subunits of LH and FSH. We have also isolated from the distal lobe and/or neurointermediate lobe of bullfrog pituitary, and determined the amino acid sequences of the substances which stimulate the release of aldosterone and corticosterone from the adrenal cortex of bullfrog … More and Xenopus laevis. One of those substances is identified as 49 amino acid residue peptide, derived from the N-terminal part of the proopiomeranocortin, termed as NPP. The other peptides are identified as Arg-vasotocin and its related peptides, termed as Hydrin-1 and Hydrin-2. We also isolated a peptide which is active in release of aldosterone from the neurointermediate lobe of pituitary gland of Xenopus having the intermediate structure of Hydrin 1 and 2, and tentatively termed as Hydrin 1・1/2. However we could not isolate the adrenocorticotropin (ACTH) during the course of the isolation of those hormones. According to the similar methods, we have isolated the ACTH from the pituitary gland of chicken. According to the similar methods, we have isolated the ACTH from the pituitary gland of chicken. Thus there is a possibility that in amphibia, NPP is the functional substitute of ACTH. The establishment of the radioimmunoassay systems are under progress.
The extract from about 1, 000 hypothalamus of the bullfrog with 1 M acetic acid is gel filtrated on Sephadex-G25 column, then chromatographed on DEAE ion-exchange column. The gonadotropin-releasing hormone (GnRH, measured by radioimmunoassay for the alpha-subunit) was eluted with 0.3-0.5M NaCl. The final isolation and identification of the GnRH is under progress. Less

Report

(3 results)
  • 1991 Annual Research Report   Final Research Report Summary
  • 1990 Annual Research Report
  • Research Products

    (46 results)

All Other

All Publications (46 results)

  • [Publications] H.Hayashi: "Characterization of chicken ACTH and α-MSH:The primary sequence of chicken ACTH is more similar to Xenopus ACTH than to other avian ACTH" Gen.Comp.Endocrinol.82. 434-443 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] H.Hayashi: "Tyrosin-130 in bullfrog throglobulin is a thyroid hormone generating site" FEBS Lett.292. 167-170 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] H.Hayashi: "Amphibian lutropin and follitropin from the bullfrog Rana catesbeiana Complete amino acid sequence of the alpha subunit" Eur.J.Biochem.203. 185-191 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] S.Iwamuro: "Arginine vasotocin(AVT)and AVT-related peptide are major aldosterone-releasing factors in the bullfrog neurointermediate lobe" Gen.Comp.Endocrinol.84. 412-418 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] S.Tanaka: "Immunological localization of the subunits of glycoprotein hormones(LH,FSH,and TSH)in the bullfrog pituitary gland using monoclonal antibodies and polyclonal antiserum" Gen.Comp.Endocrinol.77. 88-97 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] M.Sakai: "Thyrotropic activity of various adenhypophyseal hormone of the bullfrog" Zool.Sci.8. 929-934 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] H.Hayashi: "Amphibian lutropin from the bullfrog Rana catersbeiana Complete amino acid sequence of the β subunit" Eur.J.Biochem.(1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] S.Iwamuro: "Purification and characterization of joining peptide and N-terminal peptide of proopiomelanocortin from the pars distalis of the bulfrog pituitary"

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] T.Hayashi: "Simple N-linked oligosaccharides are bound to the lutopin of the bullfrog"

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] T.Hayashi: "The complete amino acid sequence of the follitropin β-subunit of the bullfrog,Rana catesbeiana" Gne.Comp.Endocrinol.

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] H.Hayashi: "The N-linked sugar chain structure bound to the bullfrog follitropin"

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Tanaka, S., Park, M. K., Hayashi, H., Hanaoka, Y., Wakabayashi, K. and Kurosumi, K.: "Immunocytochemical localization of the subunits of glycoprotein hormones in bullfrog pituitary." Gen. Comp. Endocrinol.77. 88-97 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Hayashi, H., Imai, K. and Imai, K.: "Characterization of chicken ACTH and alpha-MSH. The primary sequence of chicken ACTH is more similar to Xenopus ACTH than to other avian ACTH." Gen. Comp. Endocrinol.82. 434-443 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Iwamuro, S., Hayashi, H., Yamashita, M. and Kikuyama, S.: "AVT and AVT-related peptides are major aldosteron- releasing factors in the bullfrog neurointermadiated lobe." Gen. Comp. Endocrinol.84. 412-418 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Hayashi, H., Ohmiya, Y. and Suzuki, S.: "Tyr-130 in bullfrog thyroglobulin is a thyroid hormone generating site." FEBS Lett.92. 167-170 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Sakai, M., Hanaoka, Y., Tanaka, S., Hayashi, H. and Kikuyama, S.: "Thyrotropic activity of various adenohypophyseal hormones of the bullfrog." Zool. Sci.8. 929-934 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Hayashi, H., Hayashi, T. and Hanaoka, Y.: "Amphibian lutropin and follitropin from the bullfrog Rana catesbeiana. Complete amino acid sequence of alpha subunit." Eur. J. Biochem.203. 185-191 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Hayashi, H., Hayashi, T. and Hanaoka, Y.: "Amphibian lutropin from the bullfrog Rana catesbeiana. Complete amino acid sequence of the beta-subunit." Eur. J. Biochem.(1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Hatashi, T. Hanaoka, Y. and Hayashi, H.: "The complete amino acid sequence of the follitropin beta-subunit of the bullfrog, Rana catesbeiana." Gen. Comp. Endocrinol.(1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Iwamuro, S., Hayashi, H., Delbende, C., VAudry, H. and Kikuyama, S.: "Purification and characterization of joining peptide and N-terminal peptide of proopiomelanocortin from the pars distalis of the bullfrog pituitary." Peptides. (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Hayashi, T., Hanaoka, Y. and Hayashi, H.: "Simple N-linked oligosaccharides are bound to the lutropin of the bullfrog."

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Imai, K., Imai, K. and Hayashi, H.: "Appearance of alpha-MSH and ACTH in the pituitary gland of the developing chick embryo shown by immunocytochemistry and radioimmunoassay." Zool. Sci.7(6). 1142 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Iwamuro, S., Nomizu, H., Tanaka, S. Hayashi, H. and Kikuyama, S.: "Homologous radioimmunoassay of bullfrog joining peptide." Zool. Sci.7(6). 1143 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Hayashi, H., Ohmiya, Y. and Suzuki, S.: "Bullfrog thyroglobulin. Partial amino acid sequence and one possible T4 releasing site." Zool. Sci.7(6). 1153 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Sakai, M., Kikuyama, S., Hanaoka, Y., Tanaka, S. and Hayashi, H.: "Effect of immunoneutralization of bullfrog TSH on the T4 release" Zool. Sci.7(6). 1153 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Ohe, Y. Hanaoka, Y. and Hayashi, H.: "Amino acid sequence of Xenopus protamin." Zool. Sci.8(6). 1147 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Iwamuro, S., Hayashi, H. and Kikuyama, S.: "Occurrence of none-amidated AVT C-terminally extended with glycine-lysine in the neurointermediate lobe of Xenopus." Zool. Sci.8(6). 1167 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Kawasaki, T., Hayashi, H., Iwamuro, S. and Kikuyama, S.: "Isolation of N-fragment and CLIP from the bullfrog neurointermediate lobes" Zool. Sci.8(6). 1167 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Sakai, M., Hayashi, H., Hanaoka, Y. Tanaka, S. and Kikuyama, S.: "Purification of bullfrog TSH using immunoaffinity chromatography." Zool. Sci.8(6). 1168 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Yamasita, K., Matuda, K., Yamamoto, K., Hayashi, H., Hanaoka, Y., Tanaka, S. and Kikuyama, S.: "Isolation of Xenopus prolactin (PRL)" Zool. Sci.8(6). 1168 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] H.Hayashi: "Characterization of chicken ACTH and αーMSH:The primary sequence of Chicken ACTH is more similar to Xenopus ACTH Than to other avian ACTH" Gen.Comp.Endocrinol.82. 434-443 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] H.Hayashi: "Tyrosinー130 in bullfrog thyroglobulin is a thyroid hormone generating site" FEBS Lett.292. 167-170 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] H.Hayashi: "Amphibian lutropin and follitropin from the bullfrog Rana catesbeiana Complete amino acid sequence of the alpha subunit" Eur.J.Biochem.203. 185-191 (1992)

    • Related Report
      1991 Annual Research Report
  • [Publications] S.Iwamuro: "Arginine vasotocin(AVT)and AVTーrelaated peptide peptide are major aldosteroneーreleasing factors in the bullfrog neurointermediate lobe" Gen.Comp.Endocrinol.84. 412-418 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] S.Tanaka: "Immunological localization of the subunits of glycoprotein hormones(LH,FSH,and TSH)in the bullfrog pituitary gland using monclonal antibodies and polyclonal antiserum" Gen.Comp.Endocrinol.77. 88-97 (1990)

    • Related Report
      1991 Annual Research Report
  • [Publications] M.Sakai: "Thyrotropic activity of various adenohypophyseal hormone of the bullfrog" Zool.Sci.8. 929-934 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] H.Hayashi: "Amphibian lutropin from the bullfrog Rana catesbeiana Complete amino acid sequence of the β subunit" Eur.J.Biochem.(1992)

    • Related Report
      1991 Annual Research Report
  • [Publications] S.Iwamuro: "Purification and characterization of joining peptide and Nーterminal peptide of proopiomelanocortin from the pars distalis of the bullfrog pituiitary" Peptides.

    • Related Report
      1991 Annual Research Report
  • [Publications] T.Hayashi: "Simple Nーlinked oligosaccharides are bound to the lutopin of the bullfrog"

    • Related Report
      1991 Annual Research Report
  • [Publications] T.Hayashi: "The complete amino acid sequence of the follitropin βーsubunit of the bullfrog,Rana catesbeiana" Gen.Comp.Endocrinol.

    • Related Report
      1991 Annual Research Report
  • [Publications] H.Hayashi: "The Nーlinked sugar chain structure bound to the bullfrog follitropin"

    • Related Report
      1991 Annual Research Report
  • [Publications] 林 宏昭: "Characteization of chicken ACTH and αーMSH:The Drimavy Sequenle of chicken ACTH is More Similar to Xenopus ACTH Than to Other Auian ACTH" geu.comp.Endocrinol.82. (1991)

    • Related Report
      1990 Annual Research Report
  • [Publications] 岩室 祥一: "Arginine Vasotocin(AUT) and AUTーRelated Peptide Are Major AldosteroneーReleasing Factors in the Bullfrog Neuroindermediate Lobe" geu.comp.Endocrinol.(1991)

    • Related Report
      1990 Annual Research Report
  • [Publications] 林 宏昭: "ウシガエルのMSH" 生化学. 62. 830 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] 林 倶子: "ウシガエルの卵胞刺激ホルモンの糖鎖について" 生化学. 62. 829 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] 林 宏昭: "ウシガエルチログロブリンの部分アミノ酸配列" 日本動物学会(61回)予稿集. 140 (1990)

    • Related Report
      1990 Annual Research Report

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Published: 1990-04-01   Modified: 2016-04-21  

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