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Structure of the nephron and sites of action of hormones with special reference to hydro-mineral metabolism in the kidney of the bullfrog.

Research Project

Project/Area Number 02640586
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field 動物形態・分類学
Research InstitutionThe Nippon Dental University

Principal Investigator

UCHIYAMA Minoru  The Nippon Dental University, School of Dentistry at Niigata, Dept. of Oral Physiol. Associate Professor, 新潟歯学部, 助教授 (50095072)

Project Period (FY) 1990 – 1991
Project Status Completed (Fiscal Year 1991)
Budget Amount *help
¥1,900,000 (Direct Cost: ¥1,900,000)
Fiscal Year 1991: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 1990: ¥1,400,000 (Direct Cost: ¥1,400,000)
KeywordsBullfrog / Nephron / Hormonal receptors / Arginine vasotocin / Parathyroid hormone / Mineral metabolism / 賢ネフロン / 電解質
Research Abstract

The present study was projected to elucidate the sites of action for various hormones along the nephrons in the kidney of the bullfrog. Those hormones are arginine vasotocin (AVT), mesotocin (MST) and parathyroid hormone (PTH). On the basis of this study, morphological observation of the kidney was examined by light and electron microscopy and the reconstruction of the nephron was obtained from a serial section of the kidney of a bullfrog. Then, I used a microtechnique that allows hormone-dependent adenylate cyclase activity to be measured in samples containing a localized piece of tubules isolated by microdissection from collagenase-treated kidney tissue.
1. Morphology
Each nephron consists of the following parts : renal corpuscle, ciliated neck segment, proximal tubule, ciliated intermediate segment, distal tubule, connecting tubule, and collecting duct. The cellular ultrastructures of the early distal and the connecting tubules have resemblance to those of the mammalian thick ascendin … More g limb and of the cortical collecting tubule, respectively.
2. Methodology
Cellular production of cAMP in response to hormone was examined in microdissected segments of the nephron.
3. Effects of Hormones
(1) AVT : The basal levels of cAMP were not significantly different among the segments (renal corpuscle, proximal, distal and connecting tubules). However, the basal levels were higher in spring than in late autumn. When AVT (1 muM) was added to the incubation medium, AVT activated the production of cAMP in both distal and connecting tubules but not in the renal corpuscle and proximal tubule. However, no appreciable responses to AVT were observed in the early distal tubules from frogs collected in late autumn. These results suggest that V_2-type receptors may be present in both distal and connecting tubules of the bullfrog kidney. Seasonal differences may exist with respect to the responsiveness to AVT of the nephrons of the bullfrog.
(2) PTH : There was no stimulation of cAMP formation by PTH in both. the proximal and the distall tubules. The lack of hormonal stimulation of cAMP formation observed in both segments of tubules might reflect either the total absence of hormonal receptors associated either a cAMP-generating system in these preparations, their presence in only very small quantity, or the fact that they are not detectable by the methods used in this study.
(3) MST and calcitonin : As study of these hormones is now being carried out, enough data to get conclusion are not available. Less

Report

(3 results)
  • 1991 Annual Research Report   Final Research Report Summary
  • 1990 Annual Research Report
  • Research Products

    (19 results)

All Other

All Publications (19 results)

  • [Publications] Uchiyama,M.: "Structure of the kidney in the crab-eating frog,Rana cancrivora." Journal of Morphology. 204. 147-156 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Uchiyama,M.: "Structure of the internal gills in tadpoles of the crab-eating frog,Rana cancrivora." Zoological Science. 7. 623-630 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Uchiyama,M.: "Antagonism of responses to arginine vasotocin by its structural analogs in the bullfrog,Rana catesbeiana." General and Comparative Endocrinology. 80. 355-362 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Yoshihara,M.: "Seasonal changes in plasma calcium,in organic phosphorus and magnesium levels in adult males of the snakes,Elaphe quadrivirgata." Zoological Science. 8. 617-619 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Uchiyama,M.: "Salinity tolerance and structure of external and internal gills in tadpoles of the crab-eating frog." Cell and Tissue Research. 267. 35-44 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Uchiyama,M.: "Structure of the nephron and sites of action of arginine vasotocin in the kidney of the bullfrog." General and Comparative Endocrinology.

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Uchiyama, M., Murakami, T., Yoshizawa, H., and Wakasugi, C.: "Structure of the kidney in the crab-eating frog, Rana cancrivora." Journal of Morphology. 204. 147-156 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Uchiyama, M., Yoshizawa, H., Wakasugi, C., and Oguro, C.: "Structure of the internal gills in tadpoles of the crab-eating frog, Rana cancrivora" Zoological Science. 7. 623-630 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Uchiyama, M., and Pang, P. K. T.: "Antagonism of responses to arginine vasotocin by its structural analogs in the bullfrog, Rana catesbeiana" General and Comparative Endocrinology. 80. 355-362 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Yoshihara, M., Uchiyama, M., Murakami, T., and Yoshizawa, H.: "Seasonal changes in plasma calcium, inorganic phosphorus and magnesium levels in adult males of the snake, Elaphe quadrivirgata." Zoological Science. 8. 617-619 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Uchiyama, M., and Yoshizawa, H.: "Salinity tolerance and structure of external and internal gills in tadpoles of the crab-eating frog, Rana cancrivora." Cell and Tissue Research. 267. 35-44 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Uchiyama, M: "Structure of the nephron and sites of action of arginine vasotocin in the kidney of the bullfrog." General and Comparative Endocrinology.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Uchiyama,M.: "Structure of the kidney in the crabーeating frog,Rana cancrivora." Journal of Morphology. 204. 147-156 (1990)

    • Related Report
      1991 Annual Research Report
  • [Publications] Uchiyama,M.: "Structure of the internal gills in tadpoles of the crabーeating frog,Rana cancrivora." Zoological Science. 7. 623-630 (1990)

    • Related Report
      1991 Annual Research Report
  • [Publications] Uchiyama,M.: "Antagonism of responses to arginine vasotocin by its structural analogs in the bullfrog,Rana catesbeiana." General and Comparative Endocrinology. 80. 355-362 (1990)

    • Related Report
      1991 Annual Research Report
  • [Publications] Yoshihara,M.: "Seasonal changes in plasma calcium,in organic phosphorus and magnesium levels in adult males of the snakes,Elaphe quadrivirgata." Zoological Science. 8. 617-619 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] Uchiyama,M.: "Salinity tolerance and structure of external and internal gills in tadpoles of the crabーeating frog." Cell and Tissue Research. 267. 35-44 (1992)

    • Related Report
      1991 Annual Research Report
  • [Publications] Uchiyama,M.: "Structure of the nephron and sites of action of arginine vasotocin in the kidney of the bullfrog." General and Comparative Endocrinology.

    • Related Report
      1991 Annual Research Report
  • [Publications] Minoru Uchiyama and P.K.T. Pang: "Antagonism of Responses to Arginine Vasotocin by Its Structural Analogs in the Bullfrog,Rana catesbeiana" General and Comparative Endocrinology. 80. 355-362 (1990)

    • Related Report
      1990 Annual Research Report

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Published: 1990-04-01   Modified: 2016-04-21  

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