| Project/Area Number |
02650669
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
高分子合成
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| Research Institution | Kumamoto University |
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Principal Investigator |
HIRAYAMA Chuich Kumamoto University, Applied Chemistry, Professor, 工学部, 教授 (10040429)
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| Co-Investigator(Kenkyū-buntansha) |
SAATA Masayo Kumamoto University, Applied Chemistry, Research Associate, 工学部, 助手 (60187391)
IHARA Hirotaka Kumamoto University, Applied Chemistry, Associate Professor, 工学部, 助教授 (10151648)
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| Project Period (FY) |
1990 – 1991
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| Project Status |
Completed (Fiscal Year 1991)
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| Budget Amount *help |
¥1,800,000 (Direct Cost: ¥1,800,000)
Fiscal Year 1991: ¥400,000 (Direct Cost: ¥400,000)
Fiscal Year 1990: ¥1,400,000 (Direct Cost: ¥1,400,000)
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| Keywords | endotoxin / Bordetella pertussis / poly (gamma-methyl L-glutamate) / amino group / antigen / filamentous hemagglutinin / pertussis toxin / polyamino acid / ポリ(αーメチル Lーグルタメ-ト) / 疎水性相互作用 / アフィニティ- / 破傷風トキソィド / ポリ(γーメチル Lーグルタメ-ト) / 排除限界分子量 |
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| Research Abstract |
Preparations of adsorbents having a high affinity for endotoxins in the culture supernatant fluids of Bordetella pertussis were investigated. When poly (gamma-methyl L-glutamate) (PMLG) was used as a matrix and amino groups was used as the ligand, the highest affinity for endotoxin was attained even at high ionic strength (mu=0.2-0.4). PMLG beads. containing amino groups of about 3.2 meq/g selectively removed endotoxin in culture supernatant of B. pertussis without affecting the protective antigens. It was demonstrated that 1 ml of the wet adsorbent adsorbed 4.5 mg of endotoxin. The beads of PMLG derivatives, therefore, are considered to be useful adsorbent for the removal of endotoxin from pertussis vaccine affecting neither filamentous hemagglutinin nor pertussis toxin.
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