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Modes of Action of Dwarfing Genes on Physiological and Biochemical Characters in Rice (Oryza sativa L.)

Research Project

Project/Area Number 02660004
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field Breeding science
Research InstitutionKobe University

Principal Investigator

KAMIJIMA Osamu  Kobe University, Faculty of Agriculture, Professor, 農学部, 教授 (30031222)

Project Period (FY) 1990 – 1992
Project Status Completed (Fiscal Year 1992)
Budget Amount *help
¥2,100,000 (Direct Cost: ¥2,100,000)
Fiscal Year 1992: ¥400,000 (Direct Cost: ¥400,000)
Fiscal Year 1991: ¥200,000 (Direct Cost: ¥200,000)
Fiscal Year 1990: ¥1,500,000 (Direct Cost: ¥1,500,000)
KeywordsRice (Oryza sativa L.) / Dwarfing gene / Isogenic line / Gibberellin / IAA / Peroxidase / Lignin / RAPD / オーキシン / パ-オキシダ-ゼ / DNAマ-カ- / RAPDマ-カ-法
Research Abstract

Using a rice variety "Shiokari" and its dwarf near-isogenic lines, several experiments concerning the title of project above were carried out. The results obtained are as follows : (1) Degrees of response in shoot elongation to GA_3, inhibitors of gibberellin biosynthesis, IAA and its precursors differed among lines used. Amount of endogenous IAA extracted from shoots was fewer in Sh-d_1 and Sh-d_<18>^h than in "Shiokari". (2) Peroxidase activity in the upper two internodes of Sh-d_<13> and Sh-d_6 was remarkably high as compared with "Shiokari". Other dwarf lines also tended to show higher activity than "Shiokari". A negative correlation was observed between the peroxidase activity and the internode length especially in the second internode. (3) Lignin content was measured in the upper three internodes at various stages of growth. There was significantly negative correlation between the lignin content (%) measured at heading stage and the length of parenchyma cell measured at maturity stage. On the other hand, the lignin content (%) at maturity stage correlated positively to the internode length. (4) Random amplified polymorphic DNAs (RAPDs) were detected between "Shiokari" and three dwarf near-isogenic lines by amplification using 180 kinds of single 10-mer primers of arbitrary sequence. Among four RAPDs detected in three dwarf lines, one RAPD was subjected to investigate the segregation ratio in a F_2 population and was confirmed to segregate at a 3 : 1 ratio. A search of new RAPDs and a linkage analysis of dwarfing genes and RAPDs are now carrying aut.

Report

(4 results)
  • 1992 Annual Research Report   Final Research Report Summary
  • 1991 Annual Research Report
  • 1990 Annual Research Report
  • Research Products

    (21 results)

All Other

All Publications (21 results)

  • [Publications] 上島 脩志: "イネ第1葉の細胞長と細胞数に及ぼす矮性遺伝子の影響" 神戸大学農学部研究報告. 20. 7-14 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Shuichi FUKUOKA: "Use of random amplified polymorphic DNAs(RAPDs)for identification of rice accessions" Japanese Journal of Genetics. 67. 243-252 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] 加賀 秋人: "Random amplified polymorphic DNA(RAPD)分析法のアズキとその近縁属への適用" 神戸大学農学部研究報告. 20. 171-176 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] 平林 秀介: "矮性イネの節間におけるリグニン含量の系統間差異" 育種学雑誌.

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] 角田 重三郎: "新版 植物育種学" 文永堂, 309 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Osamu Kamijima et al.: "Effects of Dwarfing Genes on Cell Length and Cell Number in the First Leaf of Rice." Science Reports of Faculty of Agriculture, Kobe University. 20-1. 7-14 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Shuichi Fukuoka et al.: "Use of Random Amplified Polymorphic DNAs (RAPDs) for Identification of Rice Accessions." Japanese Journal of Genetics. 67-3. 243-252 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Akito Kaga et al.: "Application of Random Amplified Polymorphic DNA (RAPD) Analysis for Adzuki Been and Its Related Genera." Science Reports of Faculty of Agriculture, Kobe University. 20-2. 171-176 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Hideyuki Hirabayashi et al.: "Difference in Lignin Content in Internodes among Dwarf Near-isogenic Lines of Rice." Japanese Journal of Breeding.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] 上島 脩志: "イネ第1葉の細胞長と細胞数に及ぼす矮性遺伝子の影響" 神戸大学農学部研究報告. 20. 7-14 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] Shuichi Fukuoka: "Use of random amplified polymorphic DNAs(RAPDs)for identification of rice accessions" Japanese Journal of Genetics. 67. 243-252 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] 加賀 秋人: "Random amplified polymorphic DNA(RAPD)分析法のアズキとその近縁属への適用" 神戸大学農学部研究報告. 20. 171-176 (1993)

    • Related Report
      1992 Annual Research Report
  • [Publications] 平林 秀介: "矮性イネの節間におけるグニン含量の系統間差異" 育種学雑誌.

    • Related Report
      1992 Annual Research Report
  • [Publications] 角田 重三郎: "新版 植物育種学" 文永堂, 309 (1991)

    • Related Report
      1992 Annual Research Report
  • [Publications] 上島 脩志: "イネ第1葉の細胞長と細胞数に及ぼす矮性遺伝子の影響" 神戸大学農学部研究報告. 20. (1992)

    • Related Report
      1991 Annual Research Report
  • [Publications] Syuichi Fukuoka: "Application of Random Amplified Polymorphic DNA Method for I dentification of Rice Varieties" Japanese Journal of Genetics.

    • Related Report
      1991 Annual Research Report
  • [Publications] Kazuo Watanabe: "Effect of Dwarf Genes on the Cellular Characteristics of Coleoptile in Nearーisogenic Lines of Rice" Japanese Journal of Breeding.

    • Related Report
      1991 Annual Research Report
  • [Publications] 角田 重三郎: "新版植物育種学" 文永堂, 309 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] Kazuo Watanabe: "Effect of Dwarf Genes on the Cellular Characteristics of Coleoptile in Nearーisogenic Lines of Rice" Japanese Journal of Breeding.

    • Related Report
      1990 Annual Research Report
  • [Publications] 松尾 孝嶺: "稲学大成第3巻(遺伝編)" 農山漁村文化協会, 795 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] 角田 重三郎: "新版植物育種学" 文永堂, (1991)

    • Related Report
      1990 Annual Research Report

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Published: 1990-04-01   Modified: 2016-04-21  

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