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Molecular Study on Expression Mechanisms of Fungicide-Resistance in Botrytis cinerea

Research Project

Project/Area Number 02660041
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field 植物保護
Research InstitutionIbaraki University

Principal Investigator

AKUTSU Katsumi  Ibaraki University Agriculture Associate Professor, 農学部, 助教授 (10151002)

Co-Investigator(Kenkyū-buntansha) HIBI Tadaaki  National Institute of Agrobiological Resources(NIAR) Applied Physiology Head, 機能開発部, 室長
Project Period (FY) 1990 – 1991
Project Status Completed (Fiscal Year 1991)
Budget Amount *help
¥2,000,000 (Direct Cost: ¥2,000,000)
Fiscal Year 1991: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 1990: ¥1,500,000 (Direct Cost: ¥1,500,000)
KeywordsBotrytis cinerea / Saccharomyces cerevisiae / Benomyl-Resistance / Dicarboximide Fungicide-Resistance / PFGE / RFLP / Botrytis cinerea / Saccharomyces cerevisiae / ベンズイミダゾ-ル系薬剤耐性 / パルスフィ-ルド電気泳動 / エレクトロポレ-ション / 染色体解析 / チュ-ブリン変異遺伝子
Research Abstract

1. Mechanisms of benomyl fungicide-resistance in Botrytis cinerea. The pulsed-field gel electrophoresis (PFGE) pattern of 5 benomyl-resistant strains (IHES-3, IHES-4, CAES-7, PHDF-6 and PHGF-1) and a sensitive strain (IPCR-1) of B. cinerea were examined The four resistant strains (IHES-4, CAES-7, PHDF-6 and PHGF-1) yielded patterns that were identical to that of the sensitive strain. The resistant strain IHES-3, which contained a lot of small nuclei in the hyphae, yielded the pattern that was almost identical to that of the above strains, but still more contained a low molecular band. For all the strains analyzed, the genome size was ca. 10 Mb, with no apparent difference among the teat strains of B. cinerea. Genomic DNAs from the 6 strains were surveyed for restriction fragment length polymorphisms (RFLPs) by digestion with the restriction enzymes (BamHI, BglII, EcoRI, HindIII, NotI and ShpI) and analyzed southern blotting against a puc18-DNA, yeast-RNA or E. coli-rRNA probe labelled … More with biotin. The different patterns of rflp between the resistant and the sensitive strains was not observed. From all the strains analyzed, mitochondorial DNA (ca. 2.15kb) was Isolated, and the quantities from the resistant strains were larger than that from the sensitive strain.
2. Induction mechanisms of dicarboximide fungicide-resistance in Botrytis cinerea. Dicarboximide and/or benomyl fungicide-resistant strains (CAES and IHES series) of B. cinerea were pre-shade cultured in MS media, and after removal of the mycelia, the sensitive isolate (DS-2) of Saccharomyces cerevisiae was post-shake cultured in the respective filtrates. The DS-2 cells cultured in the filtrates were induced resistance to dicarboximide fungicides, but not to benomyl. The induced fungicide-resistance had been maintained through more than five times of consecutive subcultures. The respective patterns of PFGE and RFLP of the resistant isolates (DR-C2 and DR-C4) induced by the filtrates of B. cinerea were examined. The resistant isolates yielded patterns that were identical to that of the sensitive isolate. From the DNA band patterns by PFGE and RFLP, genetic difference the resistant and the sensitive isolates of S. cerevisiae was not found. Less

Report

(3 results)
  • 1991 Annual Research Report   Final Research Report Summary
  • 1990 Annual Research Report
  • Research Products

    (16 results)

All Other

All Publications (16 results)

  • [Publications] 入野 達之: "The Characteristics of Dicarboximide Fungicide-Resistant Strains Induced by Filtrate of the Resistant Strains of Botrytis cinerea" 日本植物病理学会報. 57. 17-23 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] 阿久津 克己: "Bioassays for Induction of Dicarboximide Fungicide-Resistance with Filtrates of the Resistant Strains of Botrytis cinerea" 日本植物病理学会報. 58. (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] 阿久津 克己: "Mechanisms of Dicarboximide Fungicide-Resistance of B.cinerea Conferred to Other Botrytis Species by Contact-Culture" 日本植物病理学会報.

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] 阿久津 克己: "Enhanced Resistance to Dicarboximide Fungicide-Resistance of Sacchromyces cerevisiae Treated with the Filtrate of the Resistant Strain of Botrytis cinerea" Appl.Environ.Microbiol.

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] 阿久津 克己: "灰色かび病菌の薬剤耐性出現機構(総説)" 植物防疫. 44. 8-13 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Tatsuyuki Irino: "The characteristics of Dicarboximide Fungicide-Resistant Strains Induced by Filtrates of the Resistant Strains of Botrytis cinerea" Ann. phytopath. Soc. Japan. 57. 17-23 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Katsumi Akutsu: "Bioassays for Induction of Dicarboximide Fungicide-Resistance with Filtrate of the Resistant Strains of Botrytis cinerea" Ann. phytopath. Soc. Japan. 58. (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Katsumi Akutsu: "Mechanisms of Dicarboximide Fungicide-Resistance of B. cinerea Conferred to Other Botrytis Species BY Contact-Culture" Ann. Phytopath. Soc. Japan. (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Katsumi Akutsu: "Enhanced Resistance to Dicarboximide Fungicide of Saccharomyces cerevisiae Treated with the Filtrate of the Resistant Strain of Botrytis cinerea" Appl. Environ. Microbiol.(1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Katsumi Akutsu: "Occurrence Mechanisms of Fungicide-Resistance in Gray mold" Plant Protection. 44. 8-13 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] 入野 達之: "The characteristic of induced dicarboximide fungicideーresistant strains conferred by postーculture in filtrates of the resistant strains of Botrytis cinerea" Annals Phytopathological Society of Japan. 57. 17-23 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] 阿久津 克己: "Bioassays for induction of dicarboximide fungicideーresistance with filtrate of the resistant strains of Botrytis cinerea" Annals Phytopathological Society of Japan. 58. (1992)

    • Related Report
      1991 Annual Research Report
  • [Publications] 阿久津 克己: "Enhanced resistant to dicarboximide fungicide to Saccharomyces cerevisiae treated with the filtrate of the resistant starin of Botrytis cinerea" Applied and Environmental Microviology.

    • Related Report
      1991 Annual Research Report
  • [Publications] 入野 達之: "The characteristics of induced dicarboximide fungicideーresistant strains conferred by postーculture in filtrates of the resistant strains of Botrytis cinerea" Annals Phytopathological Society of Japan. 57. 17-23 (1991)

    • Related Report
      1990 Annual Research Report
  • [Publications] 阿久津 克己: "Dicarboximide resistance of Botrytis cinerea conferred to Botrytis tulipae by contactーculture" Annals Phytopathological Society of Japan.

    • Related Report
      1990 Annual Research Report
  • [Publications] 阿久津 克己: "Dicarboximide resistance of Saccharomyces cerevisiae induced with filtrates of the resistant strain of Botrytis cinerea" Applied and Environmental Microbiology.

    • Related Report
      1990 Annual Research Report

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Published: 1990-04-01   Modified: 2016-04-21  

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