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In vitro culture of polyembryonic wasp embryos

Research Project

Project/Area Number 02660046
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field 植物保護
Research InstitutionTokyo University of Agriculture and Technology

Principal Investigator

IWABUCHI Kikuo  Tokyo university of Agriculture and Technology, Department of Applied Biological Sciences, Assistant, 農学部, 助手 (00203399)

Project Period (FY) 1990 – 1991
Project Status Completed (Fiscal Year 1991)
Budget Amount *help
¥2,200,000 (Direct Cost: ¥2,200,000)
Fiscal Year 1991: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 1990: ¥1,400,000 (Direct Cost: ¥1,400,000)
KeywordsPolyembryony / Parasitoid / Embryo culture
Research Abstract

Media suitable for culturing the 2-cell embryos of a polyembryonic wasp, Litomastix maculata, were exploited on more than 50 modified MGM-450 media. Bovine plasma albumin levels of more than I % was required as a component of the medium for the embryos to develop up to the complete morula. Ovalbum in and conalbumin also supported some development of the embryos, whereas lactalbumin was rather detrimental. The modifications of MGM-450 medium by changing the carbohydrates and the minerals to 0.2 5 % suct-ose alone and D-20's minerals, respectively, was required for the embryos to develop into Polygerms beyond the morula stac, . e. Experiments on the effect of supplementing- : the medium with insect hormones indicated that exogenous juvenile hormone(JH)supported the development of embryos into polygerms, which was a developmental- event specific to the polyembryonic insects. JH I was the most e erective !iorinone followed by 'H 11 and 'H Ill. On the other hand, 20-hydroxyecdysone did not. The composition of amino acids also was important for culturing 2-cell embryos up to polygerms ; amino acid composition of MGM-450 medium was the most suitable among those of several insect cell culture media. Supplementation of fetal bovine serum into the medium caused the higher percentage of Polygerm formation.
There were not clear effects of albumin and juvenile hormone where the culture was started at the morula stage ; 80-90 % of morulae cultured in either MGM-450 medium or modified MGM-450 medium mentioned above developed into polygermis.

Report

(3 results)
  • 1991 Annual Research Report   Final Research Report Summary
  • 1990 Annual Research Report
  • Research Products

    (3 results)

All Other

All Publications (3 results)

  • [Publications] Iwabuchi,K.: "Early embryonic development of a polyembryonic wasp,Litomastix maculata Ishii,in vivo and in vitro." Applied Entomology and Zoology. 26. 563-570 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Iwabuchi,K.: "Early embryonic development of a polyembiyonic wasp,Litomastix maculataIshii,invivo and in vitro." Applied Entomology and Zoology. 26. 563-570 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] Iwabuchi,K.: "Early embryonic development of polyembryonic wasp Litomastix maculata Ishii in vivo and in vitro." Applied Entomology and Zoology.

    • Related Report
      1990 Annual Research Report

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Published: 1990-04-01   Modified: 2016-04-21  

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