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Kinetic Studies on Allosteric Properties of Honeybee alpha-Glucosidase

Research Project

Project/Area Number 02660074
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field 応用生物化学・栄養化学
Research InstitutionHokkaido University

Principal Investigator

CHIBA Seiya  Hokkaido University, Faculty of Agriculture Professor, 農学部, 教授 (30001449)

Project Period (FY) 1990 – 1991
Project Status Completed (Fiscal Year 1991)
Budget Amount *help
¥2,000,000 (Direct Cost: ¥2,000,000)
Fiscal Year 1991: ¥600,000 (Direct Cost: ¥600,000)
Fiscal Year 1990: ¥1,400,000 (Direct Cost: ¥1,400,000)
KeywordsHoneybee / alpha-Glucosidase / Allosteric effect / Cooperativity / αーグルコンダ-ゼ
Research Abstract

(1)Three kinds of alpha-glucosidases(HbG-I, HbG-II and HbG-III)were purified from honeybee. In addition, an alpha-glucosidase was also purified from honey.
(2)HbG-I, II and III were immunologically confirmed to be distributed in ventriculus, hemolymph(and also ventriculus)and hypopharyngeal gland, respectively. The alpha-glucosidase from honey was identified to be the same enzyme as HbG-III.
(3)HbG-I and II showed allosteric behaviors. These two enzymes were regarded as so-called hysteretic enzyme which showed a relatively slow conformational change in second or minute order.
(4)The subsites affinities in active site of HbG-I, II and III were evaluated. Subsite 1, 2 and 3 having positive values in three enzymes were considered to be effective for binding of substrate to active site.
(5)Honeybee alpha-glucosidase, which is the same enzyme as HbG-III, was considered to be secreted into honeydew from hypopharyngeal gland.

Report

(3 results)
  • 1991 Annual Research Report   Final Research Report Summary
  • 1990 Annual Research Report
  • Research Products

    (21 results)

All Other

All Publications (21 results)

  • [Publications] Atsuo Kimura: "Allosteric Properties,Substrate Specificity andSubsite Affinities of Honeybee α-Glucosidase I" J.Biochem.107. 762-768 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Seiya Chiba: "Anomeric Configuration of Crystalline 2-Deoxymaltose" Agric. Biol. Chem.54. 3023-3024 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Hirokazu Kawagishi: "Isolation and Characterization of A lectin from Grifola frondosa Fruiting Bodies" Biochim.Biophys.Acta. 1034. 247-252 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] 松井 博和: "アイソト-プ効果によるエキソーαーグルカナ-ゼの反応機構の解析" 澱粉科学. 38. 181-185 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Hirokazu Kawagishi: "A Lectin from the Mushroom aurirella" Agric.Biol.Chem.55. 2485-2489 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Sumio Kitahata: "Mechanism of Maltal Hydration Catalyzed by β-Amylase:Role of Protein Structure in Controlling the Steric Outcome of Reaction Catalyzed by a Glycosylase" Biochemistry. 30. 6769-6775 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Akihiko Kita: "Substrate Specificity and Subsite Affinities of Crystalline α-Glucosidase from Aspergillus niger" Agric.Biol.Chem.55. 2327-2335 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Atsuo Kimura: "Allosteric Properties, Substrate Specificity and Subsite Affinities of Honeybee alpha-Glucosidase I" J. Biochem.107. 762-768 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Seiya Chiba: "Anomeric Configuration of Crystalline 2-Deoxymaltose" Agric. Biol, Chem.54. 3023-3024 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Hirokazu Kawagishi: "Isolation and Characterization of A lection from Grifola frondosa Fruiting Bodies" Biochim. Biophys. Acta. 1034. 247-252 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Hirokazu Matsui: "alpha-Secondary Isotope Effects in Reaction of Exo-alpha-Glucanases" Denpun Kagaku. 38. 181-185 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Hirokazu Kawagishi: "A Lectin from the Mushroom aurirella" Agric. Biol. Chem.55. 2485-2489 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Sumio Kitahata: "Mechanism of Maltal Hydration Catalyzed by beta-Amylase : Role of Protein Structure in Controlling the Steric Outcome of Reaction Catalyzed by a Glycosylase" Biochemistry. 30. 6769-6775 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Akihiko Kita: "Substrate Specificity and Subsite Affinities of Crystalline alpha-Glucosidase from Aspergillus niger" Agric. Biol. Chem.55. 2327-2335 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] A.Kimura: "Evidence for A Single Catalytic Site of Honeybee αーglucosidase I by Chemical modification with Diethylpyrocarbonate" J.Biochem.111. (1992)

    • Related Report
      1991 Annual Research Report
  • [Publications] A.Kita: "Substrate Specificity and Subsite Affinifies of Crystalline αーglucosidase from Asopergillus niger" Agric.Biol.Chem.55. 2327-2335 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] H.Kawagishi: "A Lectin from mushroom aurirella" Agric.Biol.Chem.55. 2485-1991 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] T.Oguma: "Purification and Substrate Specificity of αーglucosidase from Paecilomyes narioti" Biosci.Biotech.Biochem.58. (1992)

    • Related Report
      1991 Annual Research Report
  • [Publications] A.Kimura: "Allosteric Properties,Substrate Specificity and Subsitd Affinities of Honeybee αーGlucosidase I." J.Biochem.109. 762-768 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] K.Kawagishi: "Isolation and Characterization of A Lectin from Grifola frondosa Fruiting Bodies." Biochim.Biophys.Acta,. 1034. 247-252 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] S.Chiba: "Evidene for a Single Catalytic Site of Honeybee αーGlucosidase I by Chemical Modification with Diethylpyrocarbonate." J.Biochem.110. (1991)

    • Related Report
      1990 Annual Research Report

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Published: 1990-04-01   Modified: 2016-04-21  

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