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Role of Membrane Phospholipid Biosynthesis in Cellular Responce to Enviornmental Stress and Start of Cell Division Cycle in Yeast.

Research Project

Project/Area Number 02660079
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field 応用生物化学・栄養化学
Research InstitutionThe University of Tokyo (1991)
Saitama University (1990)

Principal Investigator

OHTA Akinori  The University of Tokyo, Department of Agriculture Associate Professor., 農学部, 助教授 (30125885)

Co-Investigator(Kenkyū-buntansha) TAKAGI Masamichi  The University of Tokyo, Department of Agriculture Professor., 農学部, 教授 (50018339)
SHIBUYA Isao  Saitama University, Department of Biochemistry, Professor., 理学部, 教授 (60013306)
松崎 博  埼玉大学, 理学部, 助手 (80008870)
Project Period (FY) 1990 – 1991
Project Status Completed (Fiscal Year 1991)
Budget Amount *help
¥1,900,000 (Direct Cost: ¥1,900,000)
Fiscal Year 1991: ¥600,000 (Direct Cost: ¥600,000)
Fiscal Year 1990: ¥1,300,000 (Direct Cost: ¥1,300,000)
KeywordsSaccharomyces cerevisiae / Phosphatidylserine / Phosphatidylethanolamine / Yeast / Phosphatidylserine synthase / Vacuole / Phospholipid / Biomembrane / Phosphatidylethanolamine / Saccharomyces cerevisiae / Phosphatidylserine synthase / Biomembrane / Phosphatidylinositol
Research Abstract

(1) Analysis of role of nitrogen-containing phospholipids in yeast cell growth.
(1) Response to various environmental conditions of a yeast mutant that were deficient in phosphatidylserine (PS) synthesis were examined. Even in the presence of choline or ethanolamine which enables the mutant to grow, only 10% cells were viable at mid-stationary phase, judging by Malachite green staining. Sensitivity to high concentration of Ca^<2+>, Zn^<2+>, Mn^<2+>, or basic amino acids was increased. Central vacuoles were not observed by Nomarski optics and fluorescence microscopy of quinacrine-stained cells. These results suggest that PS aynthesis is necessary for the function of intracellular membraneous structures and for cellular adaptation to environmental conditions.
(2) A strain of which chromosomal CHO1 gene that encoded PS synthase was disrupted but that carried it downstream of GAL7 promoter on a centromere plasmid was constructed. When the strain was transferred from a medium with galactose to the one with glucose as a carbon source, the amount of PS was decreased to 0.3% of total phospholipids and that of phosphatidylethanolamine was also decreased, but that of posphatidylinositol was increased up to 47% at 9 hours after C-source change. Increase in culture turbidity was continued for 9 hours after medium change, but the increase in cell number did not continue over 6 hours, when 80% to 90% of cells were unbudded cells. This result suggests that the cessation of PS synthesis blocked entry from G1 to S phase of cell division cycle.
(2) Analysis of upstream region of the CHO1 gene
(1) Expression of a CHO1-lacZ fusion gene was examined after heat shock. Since stimulation of reporter enzyme activity was not observed, role of an HSE-like sequence in 5'-upstream region was not clear.

Report

(3 results)
  • 1991 Annual Research Report   Final Research Report Summary
  • 1990 Annual Research Report
  • Research Products

    (15 results)

All Other

All Publications (15 results)

  • [Publications] S.Hamamatsu: "Regulation of Phospholipid Synthesis in Response to Nutritional Environments in Mitochondria of Saccharomyes cerevisiae" Bioscience,Biotechnology and Biochemistng. (submitted).

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] S.Hamamatsu: "Physiological CauseQuenes of Phosphatidylsarine Deficiency and Its Correlation to Vawctar Function of Sacchavomyes cerevisiae" Bioscience,Biotechnology and Biocbemistry. (submitted).

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Shioka Hamamatsu et al.: "Regulation of phospholipid synthesis in response to nutritional enviornments in mitochondria of Saccharomyces cerevisiae." Bioscience, Biotechnology and Biochemistry. (submitted).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Shioka Hamamatsu et al.: "Physiological consequences of phosphatidylserine deficiency and its correlation to vacuolar function of Saccharomyces cerevisiae." Bioscience, Biotechnology and Biochemistry. (submitted).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Shioka Hamamatsu: "Physiological consequences of phosphatidylserineーdefficiency in Saccharomyces cerevisiae." FEBS Letters.

    • Related Report
      1991 Annual Research Report
  • [Publications] Yu Fukuda: "Structural prerequisites of phosphatidylserine synthase of Saccharomyces cerevisiae for its microsomal localization." J.Biochemistry.

    • Related Report
      1991 Annual Research Report
  • [Publications] Shuichi Hiraoka: "Amplification and substantial purification of cardiolipin synthase of Escherichia coli." J.Biochemistry. 110. 443-449 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] Youichi Ohya: "Calsiumーsensitive cls mutants of Saccharomyces cerevisiar showing a pet phenotype are ascribable to deffects of vacuolar membrane." J.Biological Chemistry. 266. 13971-13977 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] 栃倉 辰六郎編,太田 明徳(分担執筆): "酵母のバイオサイエンス:伝統と新展開" 学会出版センタ-, 12 (1990)

    • Related Report
      1991 Annual Research Report
  • [Publications] 太田 明徳(分担執筆): "新生化学実験講座(4)脂質II.リン脂質" 東京化学同人, 13 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] 太田 明徳: "リン脂質合成酵素の細胞内局在性と機能調節" 遺伝. 43. 28-33 (1989)

    • Related Report
      1990 Annual Research Report
  • [Publications] Yoko Asai: "Suppression of the lethal effect of acidicーphospholipid dificiency by defective formation of the major outer membrane lipoprotein in Escherichia coli." Journal of Bacteriology. 171. 6867-6869 (1989)

    • Related Report
      1990 Annual Research Report
  • [Publications] Miyuki Fukushima: "Subcellular localization of synthetic activities for phosphatidylserine and phosphatidylinositol in Saccharomyces cerevisiae." FEBS Letters.

    • Related Report
      1990 Annual Research Report
  • [Publications] Yu Fukuda: "Structural prerequisites of yeast phosphatidylserine synthase for its microsomal localization."

    • Related Report
      1990 Annual Research Report
  • [Publications] 太田 明徳: "酵母のバイオサイエンス:伝統と新展開(栃倉 辰六郎編)pp.57ー68" 学会出版センタ-, 12 (1990)

    • Related Report
      1990 Annual Research Report

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Published: 1990-04-01   Modified: 2016-04-21  

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