| Project/Area Number |
02660080
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
応用生物化学・栄養化学
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| Research Institution | Depatment of Horticulture, Chiba University |
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Principal Investigator |
NAGATA Yoshiho Chiba University, Department of Horticulture, Professor, 園芸学部, 教授 (60013322)
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| Project Period (FY) |
1990 – 1991
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| Project Status |
Completed (Fiscal Year 1991)
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| Budget Amount *help |
¥2,100,000 (Direct Cost: ¥2,100,000)
Fiscal Year 1991: ¥600,000 (Direct Cost: ¥600,000)
Fiscal Year 1990: ¥1,500,000 (Direct Cost: ¥1,500,000)
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| Keywords | Aleuria aurantia / Lectin / Primary structrue / Secondary structure / Crystallization / レクチン / きのこ / 遺伝子発現 / Pleurotus cornucopiae |
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| Research Abstract |
1. Nucleotide sequence of Aleuria aurantia lectin(AAL)cDNA.
Complete nucleotide sequence of AAL cDNA was determined. Consequently, it was found that AAL was consisted of 312 amino acid residues, having Pro in N-terminus and Trp in C-terminus. Data of the nucleotide sequence revealed that AAL does not have the signal sequence since there was only one Met residue prior to N-terminal Pro residue.
2. Protein chemical properties of AAL.
The deduced amino- acid sequence shows that AAL- includes six internal homologous regions, and has considerable homology with a hemagglutinin from a Gram-negative bacterium, Myxococcus xanthus. Circular dichroism spectra revealed a very low content of alpha-helical and beta-sheet structures.
3. Crystallization of AAL.
AAL was crystallized and the crystals showed hexagonal bipyramid in shape. Crystallographic parameters were determined by X-ray precession photography.
4. Developmental appearance of AAL during fruiting-body formation
Crude extract prepared from the fruiting-body contained hemagglutinating activity, while that from the mycelia did not. Material that reacted with anti-AAL antibody was also included in the extract of the fruiting-body but not in that of the mycelium. These results sugested that expression of AAL gene is developmental regurated, and related to the formation of the fruiting-body in A. aurantia.
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