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Salt-induced conformational change and hydrolase susceptibility of polysaccharide responsible for floc formation in Pseudomonas sp.

Research Project

Project/Area Number 02660105
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field 応用微生物学・発酵学
Research InstitutionThe University of Tokyo

Principal Investigator

TAGO Yoshitaka  The University of Tokyo Institute of Applied Microbiology Instructor, 応用微生物研究所, 助手 (70111573)

Project Period (FY) 1990 – 1991
Project Status Completed (Fiscal Year 1991)
Budget Amount *help
¥2,000,000 (Direct Cost: ¥2,000,000)
Fiscal Year 1991: ¥600,000 (Direct Cost: ¥600,000)
Fiscal Year 1990: ¥1,400,000 (Direct Cost: ¥1,400,000)
KeywordsMucopolysaccharise / Mucopolysaccharide hydrolase / Conformation of polysaccharid / Bacterial aggregation / Ionic strength / 多糖の重合度と立体構造 / 多糖の酵素による加水分解パタ-ン
Research Abstract

The MPS hydrolase was purified with Immobiline-Canal-Isoelectric-Forcusing. The enzyme was shown one band by SDS-PAGE. The polysaccharide (MPS) purified as previously was fractionated with GPC regarding it's molecular size. Using these enzyme and polysaccharide, following results were obtained.
(1) The conformation of MPS was dependent on the ionic strength of solute. MPS was disordered state in water, and addition of salts promotes the conformational change of MPS from disordered state to a ordered state(the change of ionic strength 0 to 0.01)and packing of the form was occured during the increase of. ionic strength up to 0.05 and no change was detected more than 0.05.
(2) It was clarified by the enzyme kinetics study that the inhibition of the hydrolysis of MPS with MPS hydrolase under the ionic strength was not derived from the result of the inhibition of the enzyme, but from the results of the effects of the ionic strength to the substrate (MPS).
(3) The ordered form of MPS was seemed antiparallel arrangement and the form was accomplished in the ionic strength 0.01.
(4) The inhibition of hydrolysis of MPS with MPS hydrolase by the ionic strength was coincident with the formation of ordered form of MPS promoted with the ionic strength.
(4) The increasing of the reducing power was not detected for 20min-60min under high ionic strength using the MPS which molecular weight was higher than 10, 000-20, 000. But after that, the reducing power increased as the same rate as in the case of low ionic strength condition and low molecular weight MPS.
(5) By the enzyme products analysis by HPLC, the first product was oligo-MPS(molecular weight=10, 000-20, 000), and then, two repeating units oligosaccharide from oligo-MPS, and finally one repeating unit oligosaccharide from oligo-2 was obtained.

Report

(3 results)
  • 1991 Annual Research Report   Final Research Report Summary
  • 1990 Annual Research Report
  • Research Products

    (22 results)

All Other

All Publications (22 results)

  • [Publications] 多胡 義孝: "細胞表層高分子多糖による細菌の凝集メカニズム" 水質汚濁研究. 13. 278-282 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] 多胡 義孝: "細菌の細胞表層高分子多糖による凝集機構" 発酵工学会誌. 68. 304-306 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] 多胡 義孝: "細菌の凝集を担う細胞表層ムコ多糖の構造" 日本農芸化学会誌. 64. 521 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] 多胡 義孝: "フロック形成菌による非凝集性細菌の保持機構" 日本発酵工学会大会講演要旨集. 68 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] 多胡 義孝: "フロック形成菌Pseudomonas sp.no.12の細胞表層多糖(MPS)同士の相互作用"凝集型コンホ-メイションの形成"" 日本発酵工学会大会講演要旨集. 239 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] 多胡 義孝: "多糖の立体構造変化と加水分解酵素感受性" 日本農芸化学会誌. 66. 324 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Yoshitaka Tago: "Mechanisms of bacterial aggregation by the cell-surface polysaccharide" Japan Journla of Pollution Research. 13. 278-282 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Yoshitaka Tago: "Mechanisms of aggregation by bacterial cell-surface polysaccharide." Hakkokogaku Kaishi. 68. 304-306 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Yoshitaka Tago: "Structure of bacterial cellsurface mucopolysaccharide responsible for bacterial aggregation." Nippon Nogeikagaku Kaishi. 64. 521 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Yoshitaka Tago: "Trapping mechanisms of non-floc-culated cells by a floc-forming bacterium." Abstract of annual meeting of the Society of Fermentation Technology, Japan. 68 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Yoshitaka Tago: "Interaction between cell surface polysaccharides (MPS) of a floc-forming bacterium Pseudomonas sp. no. 12 "The conformational transition of MPS induced cell aggregation"." Abstract of anuual meeting of the Society of Fermentation Technology, Japan. 239 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Yoshitaka Tago: "Salt-induced conformational change and hydrolase susceptibility of polysaccharide responsible for floc formation in Pseudomonas sp." Nippon Nogeikagaku Kaishi. 66. 324 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] 多胡義孝: "細胞表層高分子多糖による細菌の凝集メカニズム" 水質汚濁研究. 13. 278-282 (1990)

    • Related Report
      1991 Annual Research Report
  • [Publications] 多胡義孝: "細菌の細胞表層高分子多糖による凝集機構" 醗酵工学会誌. 68. 304-306 (1990)

    • Related Report
      1991 Annual Research Report
  • [Publications] 多胡義孝: "細菌の凝集を担う細胞表層ムコ多糖の構造" 日本農芸化学会誌. 64. 521 (1990)

    • Related Report
      1991 Annual Research Report
  • [Publications] 多胡義孝: "フロック形成菌による非凝集性細菌の保持機構" 日本醗酵工学会大会講演要旨集. 68 (1990)

    • Related Report
      1991 Annual Research Report
  • [Publications] 多胡義孝: "フロック形成菌Pzeudomonas sp.no.12の細胞表層多糖(MPS)同士の相互作用“凝集型コンホ-メイションの形成"" 日本醗酵工学会大会講演・要旨集. 239 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] 多胡義孝: "多糖の立体構造変化と加水分解酵素感性" 日本農芸化学会誌. 66. 324 (1992)

    • Related Report
      1991 Annual Research Report
  • [Publications] 多胡 義孝: "細胞表層高分子多糖による細菌の凝集メカニズム" 水質汚濁研究. 13. 278-282 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] 多胡 義孝: "細菌の細胞表層高分子多糖による凝集機構" 醗酵工学会誌. 68. 304-306 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] 多胡 義孝: "細菌の凝集を担う細胞表層ムコ多糖の構造" 日本農芸化学会誌. 64. 521 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] 多胡 義孝: "フロック形成菌による非凝集性細菌の保持機構" 日本醗酵工学大会講演要旨集. 68 (1990)

    • Related Report
      1990 Annual Research Report

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Published: 1990-04-01   Modified: 2016-04-21  

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