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Studies of Microbial Mechanism for Fluoride Incorporation into Organic Compounds

Research Project

Project/Area Number 02660116
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field 応用微生物学・発酵学
Research InstitutionKyoto University

Principal Investigator

ESAKI Nobuyoshi  Institute for Chemical Research, Kyoto University, Associate Professor, 化学研究所, 助教授 (50135597)

Co-Investigator(Kenkyū-buntansha) HIRASAWA Toshiko  Institute for Chemical Research, Kyoto University, Technical Associate, 化学研究所, 教務職員
Project Period (FY) 1990 – 1991
Project Status Completed (Fiscal Year 1991)
Budget Amount *help
¥1,900,000 (Direct Cost: ¥1,900,000)
Fiscal Year 1991: ¥600,000 (Direct Cost: ¥600,000)
Fiscal Year 1990: ¥1,300,000 (Direct Cost: ¥1,300,000)
KeywordsMonofluoroacetate / Streptomyces Cattleya / Hydroxypyruvate / Fluoride Anion / C-F Bond Formation / S-Glycolyl CoA / Monofluoroethanol / Monofluorothreonine / Streptomyces Cattleya
Research Abstract

The fluoroacetate production by Streptomyces cattleya was enhanced by optimization of cultivation conditions and mutation of the producer strain. Medium K(solable starch 2.5%, soybean meal 1.5%, yeast extyract 0.2%, potassium fluoride 0.058%)was selected as the best culture medium. An improved strain Str. cattleya NTG-29 was derived from the original strain, NRRL 8057, by nitrosoguanidine treatinerit. Another strain, NTG-29-PRO-34, was derived from Str. cattlya NTG-29 through the protoplast, regeneration. In the course of improving strain, the ability of producing fluoroactate and sporulation appeared to be associted with each other. ^<14>C-Incorporation studies suggested that the precursor of fluoroacetate is a metabolite in the D-glucose assimilation pathway before pyruvate. This is based on the following flndings. Among the labeled compounds tested, radioactivity of D- ^<14>C-glucose was most efficiently inecorporated to fluoroacetate. ^<14>C-Incorporation into oxalate, L-malate and 2-oxoglutarate revealed that ^<14>C of ^<14>C-glucose followed a route which does not include pyruvate, suceingte and L-aspartate before it was incorporated to fluoroacetate. The conversion of' hydroxypyruvate to fluoroacetate by cell extract required coenzyme A, thiamine pyrophosphate, manganese chloride, while that of pyruvate did not require them. The conversion of hydroxypyruvate can be explained as follows. It is activated to O-phosphoglycolylCoA, then undergoes nucleophilic substitution reaction by fluoride anion at C-2 position followed by hydrolysis to release fluoroacetate.

Report

(3 results)
  • 1991 Annual Research Report   Final Research Report Summary
  • 1990 Annual Research Report
  • Research Products

    (8 results)

All Other

All Publications (8 results)

  • [Publications] Takashi Tamura: "Microdetermination of Fluoroacetic Acid with o-Phenylenediamine" Journal of Chromatography.

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Takashi Tamura: "Biosynthesisof Fluoroacetate in Streptomyces cattleya:Evidence of Hydroxypyruvic Acid as an lntermediate" Biosience,Biotechnology and Biochemistry.

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Takashi Tamura: "Microdetermination of Fluoroacetic Acid with o-Phenylenediamine" Journal of Chromatography.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Takashi Tamura: "Biosynthesis of Fluoroacetate in Streptomyces cattleya : Evidence of Hydroxypyruvic Acid as an Intermediate" Bioscience, Biotechnology and Biochemistry.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Takashi Tamura: "Microdetermination of Fluoroacetic Acid with oーPhenylenediamine" Journal of Chromatography.

    • Related Report
      1991 Annual Research Report
  • [Publications] Takashi Tamura: "Biosynthesisof Fluoroacetate in Streptomyces cattleya:Evidence of Hydroxypyruvic Acid as an Intermediate" Bioscience,Biotechnology and Biochemistry.

    • Related Report
      1991 Annual Research Report
  • [Publications] Takashi Tamura: "Microdetermination of Fluoroacetic Acid with oーPhenylenediamine" Journal of Chromatography.

    • Related Report
      1990 Annual Research Report
  • [Publications] Takashi Tamura: "Biosynthesis of Fluoroacetate in Streptomyces cattleya(Part I):Evidence of Hydroxypyruvic Acid as an Intermediate." Agricultural and Biological Chemistry.

    • Related Report
      1990 Annual Research Report

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Published: 1990-04-01   Modified: 2016-04-21  

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