| Budget Amount *help |
¥2,100,000 (Direct Cost: ¥2,100,000)
Fiscal Year 1991: ¥600,000 (Direct Cost: ¥600,000)
Fiscal Year 1990: ¥1,500,000 (Direct Cost: ¥1,500,000)
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| Research Abstract |
Oxygen-derived free radicals have been known to play a role in a variety of diseases such as inflammation, atherosclerosis, cerebral ischemia and cardiovascular diseases. In the course of a screening for free radical scavengers of microbial origin, an actinomycete identified as Streptomyces prunicolor was found to produce three new active substances, designated benthocyanins A, B and C. The structures of the benthocyanins were elucidated to consist of a modified phenazine chromophore with a phenyl group and a geranyl side chain based on NNM and mass spectral analysis.
Benthocyanins A, B and C inhibited lipid peroxidation in rat liver microsomes at the IC_<50>s of 0.29, 0.16 and 0.26 mug/ml, respectively. The benthocyanins also inhibited hemolysis of rat erythrocytes by a radical producer, AAPH. The IC_<50>s were 0.42 mug/ml for benthocyanin A, 0.92 mug/ml for B and 0.64 mug/ml for C.
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