| Project/Area Number |
02660145
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
製造化学・食品
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| Research Institution | Kyushu University |
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Principal Investigator |
MIYAMOTO Takahisa Kyushu Univ., Food Sci. & Technol., Assistant Professor, 農学部, 助手 (70190816)
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| Co-Investigator(Kenkyū-buntansha) |
YOSHIMOTO Makoto Kyushu Univ., Food Sci. & Technol., Associate Professor, 農学部, 助教授 (90182831)
HATANO Shoji Kyushu Univ., Food Sci. & Technol., Professor, 農学部, 教授 (30038260)
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| Project Period (FY) |
1990 – 1991
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| Project Status |
Completed (Fiscal Year 1991)
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| Budget Amount *help |
¥1,900,000 (Direct Cost: ¥1,900,000)
Fiscal Year 1991: ¥600,000 (Direct Cost: ¥600,000)
Fiscal Year 1990: ¥1,300,000 (Direct Cost: ¥1,300,000)
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| Keywords | Bacillus cereus / Sporulation / Septum formation / Temperature sensitive / DNA binding protein / Chromosome |
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| Research Abstract |
Bacillus cereus temperature-sensitive mutant ts-4 was obtained from Bacillus cereus JCM 2152 by the treatment with nitrosoguanidine. Synchronous chromosome replication was obtained in a culture of B. cereus ts-4 growing in a rich medium at 45ﾟC. At 10, 40, and 90 min from the start of chromosomal replication, samples of cells were transferred to a sporulation medium. After cultivation at 30 ﾟC for 20 hr, the sporulation rate was measured.
The capacity of B. cereus ts-4 to sporulate was highest at 40 min after chromosome replication began, and had declined at 90 min. Other cells were cultured at 30ﾟC for 120 min after the start of replication, and their chromosomes were prepared by treatment first with 10 uglml N-acetylmuramidase SG-and 2.5 mug/ml lysozyme, and later with 1% Briji 58 and 0.4% SDS. Patterns of two-dimensional electrophoresis of the chromosomal proteins from cells transferred at 10, 40, and 90 min were compared. The chromosomes of cells transferred at 40 min had greatly decreased amounts of 10 different proteins, but the amounts of three other proteins had increased. Patterns of two-dimensional electrophoresis were also compared in de novo synthesized cromosomal proteins after the start. of chromosome replication and in DNA binding proteins prepared from B. cereus DNA cellulose column. Amounts of the same 3 different proteins greatly increased in the cells transferred at 40 min in every case. These proteins seem to control the initiation of sporulation by B. cereus ts-4.
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