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Mechanism of cross-linking of casein by colloidal calcium phosphae in casein micelles

Research Project

Project/Area Number 02660146
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field 製造化学・食品
Research InstitutionFaculty of Agriculture, Kagoshima University

Principal Investigator

AOKI Takayoshi  Agriculture Biochem. Sci. & Tech. Associate Prof., 農学部, 助教授 (70034460)

Project Period (FY) 1990 – 1991
Project Status Completed (Fiscal Year 1991)
Budget Amount *help
¥1,900,000 (Direct Cost: ¥1,900,000)
Fiscal Year 1991: ¥600,000 (Direct Cost: ¥600,000)
Fiscal Year 1990: ¥1,300,000 (Direct Cost: ¥1,300,000)
KeywordsCasein / Casein micelle / Calcium phosphate / Colloidal calcium phosphate / 卵白フラボプロテイン / 人βーカゼイン
Research Abstract

Calcium and inorganic phosphate are present in excess of their Solubility and interact with casein. Calcium phosphate ity casein micelles, which is associated with casein, is called colloidal calcium phosphate(CCP). Although CCP cross-links caseins through phosphate groups, the mechanism of cross-linking has not yet been elucidated sufficiently. In the present study following results were obtained.
A small amount of the 3-P component of human beta-casein and a considerable amount of the 4-P and 5-P components were found in the CCPcross-linked casein separated from artificial casein micelles of whole human casein. CCP-cross-linked casein was formed in artificial casein micelles of tlie 3-P and 4-P components of human beta-casein with kappa-casein. In contrast, no cross-linitage was formed in artificial casein micelles of the 1-P and 2-P coutponeiits. It was concluded that at least three phosphate groups were need fGr cross-linking of casein by CCP.
CCP-cross-linked casein taras formed when calcium, phosphate and citrate were added to sodium caseinate solution. This suggested that the submicelle structure and ordered structures of casein were unnecessary for caseins to be cross-linked by CCP.
When amino groups of alpha_<s1>-casein were acetylated, citraconilated, orsuccinylated, modified alpha_<s1>-casein was somewhat more hardly cross-linked by CCP than native one.
Egg white ribofravin-binding protein is also cross-linked through its phosphate groups by CCP.

Report

(3 results)
  • 1991 Annual Research Report   Final Research Report Summary
  • 1990 Annual Research Report
  • Research Products

    (8 results)

All Other

All Publications (8 results)

  • [Publications] Takayoshi Aoki,Taketoshi Umeda and Yoshitaka Kako: "The least number of phosphate groups for crosslinking of casein by colloidal calcium phoshate." Journal of Dairy Science.

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Takayoshi Aoki,Taketoshi Umeda and Yoshitaka Kako: "Effect of the number of phosphate groups on cross-linking of casein by colloidal calcium phosphate." Bioscience,Biotechnology,and Biochemistry.

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Takayoshi Aoki,Hisayo Sakamoto and Yoshitaka Kako: "Cross-linking of caseins by colloidal calcium phosphate in the presence of urea." International Dairy Journal. 1. 67-75 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Takayoshi Aoki, Taketoshi Umeda and Yoshitaka Kako: "The least phosphate groups for crosslinking of casein by colloidal calcium phosphate" Journal of Dairy Science.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Takayoshi Aoki, Hisayo Sakamoto and Yoshitaka Kako: "Cross-linking of caseins by colloidal calcium phosphate in the presence of urea." International Dairy Journal. 1. 67-75 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Takayoshi Aoki,Hisayo Sakamoto and Yoshitaka Kako: "Crossーlinking of caseins by colloidal calcium phosphate in the presence of urea." International Dairy Journal. 1. 67-75 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] Takayoshi Aoki,Taketoshi Umeda and Yoshitaka Kato: "The least number of phosphate groups for crosslinking of casein by colloidal calcium phospate" Journal of Dairy Science.

    • Related Report
      1991 Annual Research Report
  • [Publications] Takayoshi Aoki: "Crossーlinking of caseins by colloidal calcium phosphate in the the presence of urea." International Dairy Journal.

    • Related Report
      1990 Annual Research Report

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Published: 1990-04-01   Modified: 2016-04-21  

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