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Studies on the Requirement and Nutritional Role of Marine Fish Larvae for EPA and DHA

Research Project

Project/Area Number 02660208
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field Fisheries chemistry
Research InstitutionTokyo University of Fisheries

Principal Investigator

TAKEUCHI Toshio  Tokyo University of Fisheries, Department of Aquatic Biosciences, Associate Professor, 水産学部, 助教授 (70092591)

Project Period (FY) 1990 – 1991
Project Status Completed (Fiscal Year 1991)
Budget Amount *help
¥2,200,000 (Direct Cost: ¥2,200,000)
Fiscal Year 1991: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 1990: ¥1,500,000 (Direct Cost: ¥1,500,000)
KeywordsEPA / DHA / Requirement / Nutrition / Marine Fish / Larvae / Artemia / マダイ / シマアジ / ワムシ
Research Abstract

This study was investigated the requirement and nutritional role of marine fish larvae for icosapentaenoic acid (EPA) and docosahexaenoic acid (DHA).
At first, different stages of larval fish such as striped jack, yellowtail, cod were collected and analysed their fatty acid compositions. Only DHA content in three species was decreased immediately after hatching. This result suggests that fish larvae during 5-10mm size (total length) may require high amount of DHA.
Secondly, the experiment was conducted to find out a suitable lipid type for enrichment by comparing the incorporation of lipid and n-3highly unsaturated fatty acids (n-3HUFA) in Artemia nauplii between various types of oil, such as ethylesters (EE), triglycerides (TG), methyl esters (ME), and free fatty acids (FFA) containing about 43% of n-3HUFA, using the so-called direct method. It is concluded that a maximum incorporation of n-3HUFA in Artemia can be obtained by enrichment with EE for 24h and that EE or ME are very quickly … More incorporated into TG fractions. Then these Artemia were fed on larval striped knife jaw and red sea bream to compare the dietary value of Artemia enriched with various types of oil, based on the growth, survival and chemical compositions of both fish larvae. The result indicated that the dietary value of Artemia to both species was effectively improved by enrichmewith TG, ME, and EE, especially with EE.
In next, dietary value of Artemia enriched with EE type of EPA and DHA for larval yellowtail and red sea bream was investigated. Both larval yellowtail and red sea bream fed on nauplii enriched with EPA showed good growth and high survival rate, however quite low activity tests were observed. On the other hand, fish fed DHA enriched Artemia showed high feed performances, incl. high activity tests. Thus, it is clearly indicated that larval fish require DHA in order to receive good growth and health.
Finally, two feeding experiments were conducted to compare the essential fatty acids (EFA) value between EPA and DHA in juvenile red sea bream and striped jack. Both fish fed a diet without supplemental EFA showed low feed efficiency and high morthlity. The requirement of EPA and DHA was estimated to be around 1% and 0.5% in diet for juvenile red sea bream, respectively. The EFA efficiency of DHA was also found to be twice as high as that of EPA. On the other hand, the experiment on the EFA of juvenile striped jack have demonstrated that : 1) DHA is more effective than EPA as EFA ; 2) requirement for DHA alone is about 1.7% in diet.
This study was clearly demonstrated that marine fish larvae require DHA as EFA and physiological purpose. Less

Report

(3 results)
  • 1991 Annual Research Report   Final Research Report Summary
  • 1990 Annual Research Report
  • Research Products

    (17 results)

All Other

All Publications (17 results)

  • [Publications] 竹内 俊郎: "Requirement of juvenile red seabream Pagrus major for eicosapentaenoic and docosahexaenoic acids" 日本水産学会誌. 56(8). 1263-1269 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] 竹内 俊郎: "Variety of essential fatty acids requirement in fish" 化学と生物. 29(9). 571-580 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] 竹内 俊郎: "Comparison of lipid and nー3 highly unsaturated fatty acids incorporations between Artemia enriched with various types oils by direct method" 日本水産学会誌. 58(2). 277-281 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] 竹内 俊郎: "Dietary value of Artemia enriched with various types of oils for larval striped knifejaw and red sea bream" 日本水産学会誌. 58(2). 283-289 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] 竹内 俊郎: "Supplemental effect of phospholipids and requirement of eicosapentaenoic acid and docosahexaenoic acid for juvenile striped jack" 日本水産学会誌.

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] T. Takeuchi, M. Toyota, S. Satoh, and T. Watanabe: "Requirement of juvenile red seabream Pagrus major for eicosapentaenoic and docosahexaenoic acids." Nippon Suisan Gakkaishi. 56(8). 1263-1269 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] T. Takeuchi: "Variety of Essential Fatty Acids Requirement in Fish" Kagaku to Seibutu. 29(9). 571-580 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] T. Takeuchi, M. Toyota, and T. Watanabe: "Comparison of lipid and n-3 highly unsaturated fatty acids incorporations between Artemia enriched with various types of oils by direct method." Nippn Suisan Gakkaishi. 58(2). 277-281 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] T. Takeuchi, M. Toyota, and T. Watanabe: "Dietary value of Artemia enriched with various types of oils for larval striped knifejaw and red sea bream." Nippon Suisan Gakkaishi. 58(2). 283-289 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] T. Takeuchi, T. Arakawa, S. Satoh, and T. Watanabe: "Supplemental effect of phospholipids and requirement of eicosapentaenoic acid and docosahexaenoic acid for juvenile striped jack" Nippon Suisan Gakkaishi.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] 竹内 俊郎: "魚類における必須脂肪酸要求の多様性" 化学と生物. 29(9). 571-580 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] Toshio Takeuchi: "Comparison of lipid and nー3 highly unsaturated fatty acids incorporations between Artemia enriched with various types of oils by direct method" Nippon Suisan Gakkaishi. 58(2). 277-281 (1992)

    • Related Report
      1991 Annual Research Report
  • [Publications] Toshio Takeuchi: "Dietary value of Artemia enriched with various types of oils for larval striped knifejaw and red sea bream" Nippon Suisan Gakkaishi. 58(2). 283-289 (1992)

    • Related Report
      1991 Annual Research Report
  • [Publications] Toshio Takeuchi: "Supplemental effect of phospholipids and requirement of eicosapentaenoic acid and docosahexaenoic acid for juvenile striped jack" Nippon Suisan Gakkaishi. (1992)

    • Related Report
      1991 Annual Research Report
  • [Publications] 竹内 俊郎: "「魚類における栄養素の欠乏症と要求量」平成3年度栽培漁業技術研修事業基礎理論コ-ス" 水産庁(日本栽培漁業協会), 68 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] 竹内 俊郎: "マダイ稚魚のEPA及びDHA要求量(英文)" 日本水産学会誌. 56. 1263-1269 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] 竹内 俊郎: "魚類における必須脂肪酸要求の多様性" 化学と生物. (1991)

    • Related Report
      1990 Annual Research Report

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Published: 1990-04-01   Modified: 2016-04-21  

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