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Biochemical Analysis of The Early Japanese Quail's Embryos Showing Inbreeding Depression

Research Project

Project/Area Number 02660277
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field 畜産学(含草地学)
Research InstitutionOkayama University

Principal Investigator

SATO Katsunori  Okayama University, Faculty of Agriculture, Professor, 農学部, 教授 (10033325)

Project Period (FY) 1990 – 1991
Project Status Completed (Fiscal Year 1991)
Budget Amount *help
¥900,000 (Direct Cost: ¥900,000)
Fiscal Year 1991: ¥400,000 (Direct Cost: ¥400,000)
Fiscal Year 1990: ¥500,000 (Direct Cost: ¥500,000)
KeywordsInbreeding depression / Early embryo / Japanese Quail / Biochemistry / Enzyme Activity / Free amino acid / Nucleaside / Protein / グルコ-ス6ーリン酸デヒドロゲナ-ゼ / アミノ酸の取り込み / ヌクレオシドの取り込み
Research Abstract

In order to determine the cause of inbreeding depression in the early developmental stage in Japanese quail, free amino acids of yolk, enzyme activities, contents of protein and nucleic acids, and incorporations of amino acids and nucleoside(thymidine, uridine)into embryos were analyzed biochemically in this study. Prior to biochemical examination, embryonic mortality was examined. The marked high embryonic mortality in the early developmental stage was found in the full-sib mating group and increased remarked with inbreeding. Furthermore, the decrease in the cell division and proliferation of the early embryos was found in the full-sib mating group by morphological examination. The free amino acids of yolk in the full-sib mating group were lower in contents than random mating group, showing the large differences at the 3rd generation.
Hexokinase, phosphorylase and glucose 6-phosphate dehydrogenase activities were significantly lower in the full-sib mating group and decreased with inbreeding. The relationships between these enzyme activities and embryonic development were found in the two mating groups. The marked reduced contents of protein, DNA and RNA were observed in the full-sib mating group when compared with random mating group. Lower values of protein/DNA and RNA/DNA in 2 days embryos were also found in the full-sib mating group. Incorporations of ^<14>C-glycine, leucine, glutamic acid, and ^3H-thymidine and uridine into embryos were depressed in the full-sib mating group. RNA analysis of the early embryos did not succeed because of technical problem and will be advanced further.
These results indicate that the increase of embryonic mortality and retardation in the full-sib mating group might be caused by the lack of free amino acids and the decrease in the metabolic function related to enzyme, protein and nucleis acid synthesis.

Report

(3 results)
  • 1991 Annual Research Report   Final Research Report Summary
  • 1990 Annual Research Report
  • Research Products

    (16 results)

All Other

All Publications (16 results)

  • [Publications] Hedianto,Y.E.,K.Sato & T.Ino: "Embryonic development in inbred and hybrid quail" Anim.Sci.Technol.(Jpn).(日本畜産学会報). 62. 7-11 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Hedianto,Y.E.,K.Sato,Y.Kawamoto & T.Ino: "Mitotic activity in the inbred and hybrid quail embryos" Anim.Sci.Technol.(Jpn).62. 651-653 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] 佐藤 勝紀: "ニホンウズラにおける近交退化現象" 重水素同位体研究会報. 2. 38-40 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Sato,K.,Y.Ogawa & T.Ino: "Protein and nucleic acids in the inbred quail embryos" Anim.Sci.Technol.(Jpn).

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Sato,K.,S.Fujimoto & T.Ino: "Hexiokinase and phosphorylase activities in the inbred quail embryos" Anim.Sci.Technol.(Jpn).

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Hedianto, Y. E., K. Sato and T. Ino: "Embryonic development in inbred and hybrid quail" Anim. Sci. Technol. (Jpn). 62. 7-11 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Hedianto, Y. E., K. Sato, Y. Kawamoto and T. Ino: "Mitotic activity in the inbred and hybrid quail embryos" Anim. Sci. Technol. (Jpn). 62. 651-653 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Sato, K.: "Inbreeding depression in Japanese quail" J. Deuterium compound in Medical Science. 2. 38-40 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Sato, K., Y. Ogawa and T. Ino: "Protein and nucleic acids in the inbred quail embryos" Anim. Sci. Technol. (Jpn).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Sato, K., S. Fujimoto and T. Ino: "Hexiokinase and phosphorylase activities in the inbred quail embyos." Anim. Sci. Techmol. (Jpn).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Hedianto,Y.E.,K.Sato & T.Ino: "Embryonic development in inbred and hybrid quail" Anim.Sci.Technol.(日本畜産学会報). 62. 7-11 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] Hedianto,Y.E.,K.Sato,Y.Kawamoto & T.Ino: "Mitotic activity in the inbred and hybrid quail embryos" Anim.Sci.Technol.(Jpn).62. 651-653 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] 佐藤 勝紀: "ニホンウズラにおける近交退化現象" 重水素同位体研究会報. 2. 38-40 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] Sato,K.,Y.Ogawa & T.Ino: "Protein and nucleic acids in the inbred quail emb" Anim.Sci.Technol.(Jpn).

    • Related Report
      1991 Annual Research Report
  • [Publications] Sato,K.,S.Fujimoto & T.Ino: "Hexiokinase and phosphorylase activities in the inbred quail embryos" Anim.Sci.Technol.(Jpn).

    • Related Report
      1991 Annual Research Report
  • [Publications] 佐藤 勝紀・藤本 佐恵子・猪 貴義: "ニホンウズラの近交初期胚におけるヘキソキナ-ゼとホスホリラ-ゼの酵素活生値" 日本家離学会誌.

    • Related Report
      1990 Annual Research Report

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Published: 1990-04-01   Modified: 2016-04-21  

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