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Involvement of Membrane Skeletal Proteins in the Stimulus-Response Coupling of the Neutrophil and Its Molecular Mechanism

Research Project

Project/Area Number 02670008
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field General anatomy (including Histology/Embryology)
Research InstitutionKyoto University

Principal Investigator

FUJIMOTO Toyoshi  Kyoto Univ., Fac. of Med. Associate Professor, 医学部, 助教授 (50115929)

Project Period (FY) 1990 – 1991
Project Status Completed (Fiscal Year 1991)
Budget Amount *help
¥2,300,000 (Direct Cost: ¥2,300,000)
Fiscal Year 1991: ¥1,200,000 (Direct Cost: ¥1,200,000)
Fiscal Year 1990: ¥1,100,000 (Direct Cost: ¥1,100,000)
KeywordsNeutrophil / Fodrin / Band 4.1 / Chemotaxis / Cell Motility / Membrane skeleton / Immunocytochemistry / Electron Microscopy / 膜裏打ちタンパク質
Research Abstract

1. By preembedding immunoelectron microscopy, most(>75%)of fodrin in the normal human neutrophil was not seen on the cell membrane nor within 100 nm from it, but in the filamenlous cytoplasm further inside. This result was distinct from those with speclrin in the erythrocyte and fodrin in the lymphocyte, in which a majority of immunogold labeling was attached to the cell membrane. The result indicates that fodrin in the neutrophil is a cytoskeletal protein rather than a peripheral membrane Drotein.
2. Neutrophils treated with a chemotactic peptide, f-Met-Leu-Phe, became morphologically differential"led along the front-tail axis, and fodrin became concentrated in the posterior region ; the percentage of cells showing the polarized distribution of fodrin was more than 70% at 2 min, reached the maximum of around 80% at 10 min at room temperature, and decreased thereafter. The drastic redistribution occurs much faster than the similar relocation of fodrin in capping lymphocytes, which generally takes more than 10 min at 37゚C.
3. The superior cell membrane of adherent neutrophils was removed and the remaining basal half of the cells was labeled for immunogold electron microscopy(unroofing method). In unstimulated cells, immunolabeling was observed uniformly in the membrane and filaments of 5 nm in diameter were labeled occasionally ; after stimulation with the chemotactic peptide, labeling was concentrated in the posterior half of the cell where numerous bundles of 10 nm filaments run in parallel. The result indicates that fodrin mediates'the redistribution of cytoskeletal components in the neutrophil.
4. Similar dynamic redistribution was observed in the epidermis and the corneal epithelium. The unroofing method was soplied to the chromaffin cell and filaments of 2-3 nm in diameter were found labeled for fodrin.

Report

(3 results)
  • 1991 Annual Research Report   Final Research Report Summary
  • 1990 Annual Research Report
  • Research Products

    (28 results)

All Other

All Publications (28 results)

  • [Publications] Toyoshi Fujimoto: "Fodrin in the human polymorphonuclear leucocyte:redistribution induced by the chemotactic peptide" Journal of Cell Science. 96. 477-484 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] 藤本 豊士: "ヒト好中球のバンド4.1タンパク質" 解剖学雑誌. 65. 265 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Kozo Yoneda: "Distribution of fodrin in the keratinocyte in vivo and in vitro" The Journal of Investigative Dermatology. 94. 724-729 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Toyoshi Fujimoto: "Dynamic translocation of fodrin:immunoelectron microscopy using 1 nm colloidal gold-conjugated antibody and silver enhancement" Journal of Electron Microscopy. 39. 330 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] 藤本 豊士: "フオドリンの局在とその機能" 電子顕微鏡. 25. 152-158 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Toyoshi Fujimoto: "Immunocytochemical Localization of fodrin and ankyrin in bovine chromaffin cells in vitro" The Journal of Histochemistry and Cytochemistry. 39. 1485-1493 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Toyoshi Fujimoto: "Immunoelectron microscopy of fodrin in the unroofed specimen of the adherent human neutrophil" Acta Histochemica et Cytochemica. 25. (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Masayo Takahashi: "Distributional change of fodrin in the wounded corneal epithelium in vivo" Investigative Ophthalmology and Visual Science.

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Toyoshi Fujimoto: "Electron Microscopic Cytochemistry and Immunocytochemistry in Biomedicine,eds.by K.Ogawa and T.Barka" CRC Press,Inc.,Boca Raton,

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] 藤本 豊士: "組織細胞化学1990,日本組織細胞化学会編" 学際企画,東京, 212 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Toyoshi Fujimoto: "第六回日中電子顕微鏡学セミナ-予稿集" 日中電子顕微鏡学セミナ-実行委員会, 125 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Toyoshi Fujimoto: "Fodrin in the human polymorphonuclear leukocyte ; redistribution induced by the chemotactic peptide" Journal of Cell Science. 96. 477-484 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Toyoshi Fujimoto: "Band 4.1 Protein in the human neutrophil" Acta Anatomica Nipponica. 65. 265 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Kozo Yoneda: "Distribution of fodrin in the Keratinocyte in vivo and in vitro" The Journal of Investigative Dermatology. 94. 724-729 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Toyoshi Fujimoto: "Dynamic tranlocation of fodrin : immunoelectron microscopy using 1 nm colloidal gold-conjugated antibody and silver enhancement" Journal of Electron Microscopy. 39. 330 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Toyoshi Fujimoto, Fodrin: "Distribution and function" Denshikenbikyou. 25. 152-158 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Toyoshi Fujimoto: "Immunocytochemical localization of fodrin and ankyrin in bovine chromaffin cells in vitro" The Journal of Histochemistry and Cytochemistry. 39. 1485-1493 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Toyoshi Fujimoto: "Immunoelectron microscopy of fodrin in the unroofed specimen of the adherent neutrophil" Acta Histochemica et Cytochemica.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Masayo Takahashi: "Distributional change of fodrin in the wounded corneal epithelium in vivo" Investigative Ophthalmology and Visual Science.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Toyoshi Fujimoto: "Cytoskeleton, in Electron Microscopic Cytochemistry and Immunocytochemistry in Biomedicine, eds. by K. Ogawa and T. Barka" CRC Press, Inc., Boca Raton.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] 藤本 豊士: "フォドリンの局在とその機能" 電子顕微鏡. 25. 152-158 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] Toyoshi Fujimoto: "Immunocytochemical localization of fodrin and ankyrin in bovine chromaffin cells in vitro" The Journal of Histochemistry and Cytochemistry. 39. 1485-1493 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] Toyoshi Fujimoto: "Immunoelectron microscopy of fodrin in the unroofed specimen of the adherent human neutrophil" Acta Histochemica et Cytochemica. 25. (1992)

    • Related Report
      1991 Annual Research Report
  • [Publications] Masayo Takahashi: "Distributional change of fodrin in the wounded corneal epithelium in vivo" Investigative Ophthalmology and Visual Science. (1992)

    • Related Report
      1991 Annual Research Report
  • [Publications] Toyoshi Fujimoto: "第六回日本電子顕微鏡学セミナ-予稿集" 日中電子顕微鏡学セミナ-実行委員会, 125 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] Toyoshi Fujimoto: "Electron Microscopic Cytochemistry and Immunocytochemistry in Biomedicine,eds.by K.Ogawa and T.Barka" CRC Press,Inc.,Boca Raton,

    • Related Report
      1991 Annual Research Report
  • [Publications] T.Fujimoto: "Fodrin in the human polymorphonuclear leukocyte:redistribution induced by the chemotactic peptide." Journal of Cell Science. 96. 477-484 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] 藤本 豊士: "ヒト好中球のバンド4.1タンパク質" 解剖学雑誌. 65. 265 (1990)

    • Related Report
      1990 Annual Research Report

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Published: 1990-04-01   Modified: 2016-04-21  

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