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The Observation of the cells expressing superoxide dismutase mRNA by in situ hybridization.

Research Project

Project/Area Number 02670011
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field General anatomy (including Histology/Embryology)
Research InstitutionOkayama University

Principal Investigator

SASAKI Junzo  Okayama Univ. Med. Sch. Dept. Anat. Professor, 医学部, 教授 (30093686)

Project Period (FY) 1990 – 1991
Project Status Completed (Fiscal Year 1991)
Budget Amount *help
¥1,500,000 (Direct Cost: ¥1,500,000)
Fiscal Year 1991: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 1990: ¥1,000,000 (Direct Cost: ¥1,000,000)
KeywordsInsitu Hybridization / SOD / Superoxide / RNA Probe / Actin / Digoxigemin / ジゴキシゲニン
Research Abstract

Recent studies show that there is a close relationship between reactive oxygen species and man diseases.
In the first year, we synthesized ^<35>S-labeled RNA probe from cDNA of human Cu/Zn-type superoxide dismutase(SOD), which degrades reactive oxygen species and investigated the expression of SOD in normal rat tissues by in situ hybridization(ISH). In addition, ISH using nonradioactive probes was examined. As a resuly, the signals showing the presence of SOD mRNA were found in hippocampus, Purkinje's cells incerebellum, hepatocytes and epithelial cells of the bronchioles. Eosinophils also showed strong signals. This is interesting finding because, eosinophils are potent producer of active oxygen species. Nonradioactive RNA probes were synthesized in the presence of digoxigenin(DIG)-UTP and were visualized with anti-DIG-alkaline phosphatase conjugate and alkaline phosphatase reaction. Weak signals of SOD mRNA were detected in the isolated eosinophils while the sigals of actin mRNA were detected under electron microscopy by nonradioactive ISH.
In the last year, the expression of SOD mRNA was examined under pathological consitions. First, we tested the effect of a SOD derivative(SM-SOD)on liver injury caused by ischemia and repurfusion under electron microscopy. Postischemic reperfusion caused a drastic change in liver morphology, and intravenous administration of SM-SOD completely inhibited these morphological changes. But, the expression of endogeneous SOD in hepatocytes under these conditions was not marked. Secondly, we tested the exppession of SOD in rat lung after prolonged oxygen inhalation which caused lethal lung edema. The expression of SOD is not marked. It may be necessary to study the change of the expression of Mn type SOD.

Report

(3 results)
  • 1991 Annual Research Report   Final Research Report Summary
  • 1990 Annual Research Report
  • Research Products

    (42 results)

All Other

All Publications (42 results)

  • [Publications] Yoshiki Takehara: "Changes in the levels of lipoperoxide and antioxidant factors in human placenta during gestation." Acta Med.Okayama. 44. (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Yoshitaka Fujii: "Fetal calf serum increased the zona pellucida penetrability of rat oocytes matured in vitro." Acta Med.Okayama. 44. (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Junzo Sasaki: "Synthesis of actin in the nonciliated bronchiolar epithelial(Clara)colls of perinatal rats demonstrated by electron microscopy and in situ hybridization." Acta Histochem.Cytochem.23. (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Masayasu Inoue: "Inhibition of oxygen toxicity by targeting superoxide dismutase to endothelial cell surface." FEBS Lett.269. (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Masayasu Inoue: "Targeting SOD by gene and protein engineering and inhibition of free radical injury." Free Rad.Res.Commus.12-13. 391-399 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Sadahiro Watanabe: "Immunofluorescence microscopic study on the cytoskeletal filaments in the separated superficial cells of rat urinary bladder epithelium." Acta Histochem.Cytochem.24. (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Junzo Sasaki: "The expression of actin mRNA in megakaryocytes demonstrated in paraffin sections,and Epoxy resin semi-thin and thin sections by in situ hybridization." J.Electron Microsco.40. 118-123 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Junzo Sasaki: "The localization of actin mRNA in megakaryocytes demonstrated by in situ hybridization using digoxigenin-labeled riboprobes." Acta Histochem.Cytochem.24. (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] 佐々木 順造: "放射性および非放射性標識リボプロ-ブによるin situハイブリダイゼ-ション法" 岡山医学会雑誌. 103. (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] 井上 正康: "酵素活性ペプチドの分子設計と生体代謝制御。" BME(医用電子と生体工学). 5. (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Masayasu Inoue: "Expression of a hybrid Cu/Zn-type superoxide dismutase which has high affinity for heparin-like proteoglycans on vascular endothelial cells." J.Biol.Chem.266. (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Koichi Nakazono: "Does superoxide underlie the pathogenesis of hypertension?" Proc.Natl.Acad.Sci.USA. 88. 10045-10048 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Sadahiro Watanabe: "12-O-tetradecanoylphorbol-13-acetate(TPA)induces selective desquamation of superficial cells in rat urinary bladder epithelium." Cell Tiss.Res.(1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Sato E.: "Overexpression in yeast of neutrophil specific recombinant 39kDa protein,annexin I,and its biochemical properties.Nobel calcium-binding proteins-Fundamentals and clinical implications.(Heizmann,C.W.ed.)" Springer, (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Inoue,M.: "Inhibition of oxugen toxicity by regulating active oxygen metabolism in and around vascular endothelial cells with site-directed SOD derivatives.Oxidative Damage and Repair(Davies,K.ed.)" Pergamon Press, (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Yoshiki Takehara, Tamotsu Yoshioka, and Junzo Sasaki: "Changes in the levels of lipoperoxide and antioxidant factors in numan Placenta during gestation" Acta Med. okayama. 44. (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Yoshitaka Fujii, Tamotsu Yoshioka and junzo Sasaki: "Fetal calf calf serum increased the zona pellucida penetrability of rat oocytes matured in vitro." Acta Med. Okayama. 44. (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Junzo Sasaki, Sadahiro Watanabe, Takako Nomura, Shigeto Kanda, Tomoichiro Yamaai, Eiki Koyama, Sumihare Noji and Shigehiko Taniguchi: "Synthesis of actin in the nonciliated bronchiolar epithelial (Clara) cells of perinatal rats demonstrated by electron microscopy and in situ hybridization." Acta Histochem. Cytochem.23. (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Masayasu Inoue, Nobukazu Watanabe, Yoshimasa Morino, Yoshikazu Tanaka, Teruo Amachi and Junzo Sasaki: "Inhibition of oxygen toxicity by targeting superoxide dismutase to endothelial cell surface." FEBS LEtt. 269. (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Masayasu Inoue, Nobukazu Watanabe, Toshihiko Utsumi and Junzo Saski: "Targeting SOD by gene and protein engineering and inhibition of free radical injury." Free Rad. Res. Commus.12-13. 391-399 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Sadahiro Watanabe, Junzo Sasaki, Toshiyuki Kouno, Takao Okada and Nagayasu Otsuka: "Immunofluorescence microscopic study on the cytoskeletal filaments in the separated superficial cells of rat urinary bladder epithelium." Acta Histochem. Cytochem.24. 21-27 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Junzo Sasaki, Takako Nomura, Sadahiro Watanabe, Shigeto Kanda, Hiroki Watanabe, Kiyoshi Miake, Sumihare Noji and Shigehiko Taniguchi: "The expression of actin mRNA in megakaryocytes demonstraed in paraffin sections, and Epoxy resin semi-thin and thin sections by in situ hybridization." J. Electron Microsco.40. 118-123 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Junzo Sasai, Takako Nomura, Sadahiro Watanabe, Shigeto Kanda, Toshiyuki Kouno, Takao Okada, Sumihare Noji and Hiroki Watanabe: "The localization of actin mRNA in megakaryocytes demonstrated by in situ hybridization using digoxigenin-labeled riboprobes." Acta Histochem. Cytoche.24. (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Masayasu Inoue, Nobukazu Watanabe, Kenji Matsuno, Junzo Sasaki, Yoshikazu Tanaka, Haruyo Hatanaka and Teruo Amachi: "Expression of a hybrid Cu/Zu-type superoxide dimutase which has high affinity for heparin-like proteoglycans on vascular endothelial cells." J. Biol. Chem.266. (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Koichi Nakazono, Nobukazu Watanabe, Kenjiro Matsuno, Junzo Sasaki, Tatsuo Sato and Masayasu Inoue: "Does superoxide underlie the pathogenesis of hypertension?" Proc, Natl. Acad. Sci. USA. 88. 10045-10048 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Sadahiro Watanabe and junzo Sasaki: "12-O-tetradecanoylphorbol-13-acetate (TPA) induces selective desquamation of superficial cells in rat urinary bladder epithelium." Cell Tiss. Res.(1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Sato E. Tanaka, Y. Edashige, K. Sasaki J. Inoue, M. & Utsumi, K: "Overexpression in yeast of neutrophil specific recombinant 39kDa protein, annexin I, and its biochemical properties." Nobel calcium-binding proteins-Fundamentals and clinical implications.Springer. (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Inoue, M. Watanabe, K. Nakazono, K. and Sasaki, J.: "Inhibition of oxygen toxicity by regulating active oxygen metabolism in and around vascular endothelial cells with site-directed SOD derivatives." Oxidative Damage and Repair. Pergamon Press.(1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] JUNZO SAKAI: "THE EXPESSION OF ACTIN mRNA IN MEGAKARYOCYTES DEMONSRATED IN PARAFFIN SECTIONS,AND EPOXY RESIN SEMIーTHIN AND THIN SECTIONS BY IN SITU HYBRIDIZATION." J.ELECTRON MICROSCOPY. 40. 118-123 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] JUNZO SAKAI: "THE LOCALIZATION OF ACTIN mRNA IN MEGAKARYOCYTES DEMONSTRATED BY IN SITU HYBRIDIZATION USING DIGOXIGENINーLABELED RIBOPROBES." ACTA HISTOCHEM.CYTOCHEM.24. 335-339 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] 佐々木 順造: "放射性および非放射性標識リボプロ-ブによるIn situハイブリダイゼ-ション法" 岡山医学会雑誌. 103. 1-10 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] MASAYASU INOUE: "EXPRESSION OF A HYBRID Cu/ZnーTYPE SUPEROXIDE DISMUTASE WHICH HAS HIGH AFFINITY FOR HEPARINーLIKE PROTEOGLYCANS ON VASCULAR ENDOTHELIAL CELLS." J.BIOL.CHEM.266. 16409-16414 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] KOICHI NAKAZONO: "DOES SUPEROXIDE UNDERLIE THE PATHOGENESIS OF HYPERTENSIO?" PROC.NATL.ACAD.SCI.USA. 88. 10045-10048 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] SADAHIRO WATANABE: "12ー0ーTETRADECANOYLPHORBOLー13ーACETATE INDUCES SELECTIVE DESQUAMATION OF SUPERFICIAL CELLS IN RAT URINARY BLADDER EPITHELIUM." CELL TISSUE RES.(1992)

    • Related Report
      1991 Annual Research Report
  • [Publications] JUNZO SASAKI: "SYNTHESIS OF ACTIN IN THE NONCILIATED BRONCHIOLAR EPITHELIAL(CLARA)CELLS OF PERINATAL RATS DEMONSTRATED BY ELECTRON MICROSCORY AND IN SITU HYBRIDIZATION^*" Acta Histochem.Cytochem.23. 453-465 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] Masayasu Inoue: "Inhibition of oxygen toxicity by targeting superoxide dismutase to endothelial cell surface" FEBS LETTER. 269. 89-92 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] SADAHIRO WATANABE: "IMMUNOFLUORESCENCE MICROSCOPIC STUDY ON THE CYTOSKELETAL FILAMENTS IN THE SEPARATED SUPERFICIAL CELLS OF RAT URINARY BLADDER EPITHELIUM" Acta Histochem.Cytochem.24. 21-27 (1991)

    • Related Report
      1990 Annual Research Report
  • [Publications] Junzo SASAKI: "The Expression of Actin mRNA in Megakaryocytes Demonstrated in Paraffin Sections,and Epoxy Resin SemiーThin and Thin Sections by In Situ Hybridization." J.Electron Miuroscopy. 40. (1991)

    • Related Report
      1990 Annual Research Report
  • [Publications] JUNZO SASAKI: "THE LOCALIZATION OF ACTIN mRNA IN MEGAKARYOCYTES DEMONSTRATED BY IN SITU HYBRIDIZATION USING DIGOXIGENINーLABELED RIBOPROBES." Acta.Histochem.Cytochem.24. (1991)

    • Related Report
      1990 Annual Research Report
  • [Publications] 佐々木 順造: "放射性および非放射性標識リボプロ-ブによるIn situハイブリダイゼ-ション法" 岡山医学会雑誌. 103. (1991)

    • Related Report
      1990 Annual Research Report
  • [Publications] Eisuke Sato: "OVEREXPRESSION IN YEAST OF NEUTROPHLL SPECIFIC RECOMBINANT 39kDa PROTEIN,ANNEXIN I,AND ITS BIOCHEMICAL PROPERTIES in Novel CalciumーBinding Proteins." Springer, (1991)

    • Related Report
      1990 Annual Research Report
  • [Publications] Masayasu Inoue: "INHIBITION OF OXYGEN TOXICITY BY REGULATING ACTIVE OXYGEN METABOLISM IN AND AROUND VASCULAR ENDOTHELIAL CELLS WITH SITEーDIRECTED SOD DERIVATIVES in Oxidative Damage and Repair" Pergamon Press, (1991)

    • Related Report
      1990 Annual Research Report

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Published: 1990-04-01   Modified: 2016-04-21  

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