| Project/Area Number |
02670025
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
神経解剖学
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| Research Institution | Tsukuba University |
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Principal Investigator |
KOHNO Kunio Basic Med.Sci.,Anat., Prof., 基礎医学系, 教授 (60013942)
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| Project Period (FY) |
1990 – 1992
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| Project Status |
Completed (Fiscal Year 1992)
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| Budget Amount *help |
¥2,400,000 (Direct Cost: ¥2,400,000)
Fiscal Year 1992: ¥400,000 (Direct Cost: ¥400,000)
Fiscal Year 1991: ¥400,000 (Direct Cost: ¥400,000)
Fiscal Year 1990: ¥1,600,000 (Direct Cost: ¥1,600,000)
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| Keywords | neurons / polarity / microtubules / serial sections / image processing / axon / dendrites / 滑面小胞体 / 固定法 / 老化 / イノシトール三燐酸 / ゴルジ装置 / 軸索初節 / 電子顕微鏡 |
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| Research Abstract |
Vertebrate neurons regularity have two definite kinds of processes, axon and dendrites, both of which need different functional materials for transmission and reception, but contain no organelles for synthesis of materials. The materials will be synthesized in ER and Golgi apparatus of neuronal soma and distributed for the axon and dendrites. The mechanism of distribution for both processes has been examined in the morphological viewpoint.
1) First project year: It was revealed that goldfish Purkinje cell cytoplasm has a peripheral rim of Nissl body and a central Golgi area, and a bundle of axonal microtubules penetrates deeply into the Golgi area.
2) Second year: Three-dimensional relationships between the bundle of axonal microtubules and the Golgi networks were examined in 10 neurons reconstructed from serial ultrathin sections.
3) Third year: Lamellar stacks of smooth endoplasmic reticulum contained in the Purkinje cell dendrites and in which the localization of IP3 receptors is reported, were reconstructed from the serial ultrathin sections.
As a result, it can be said that each neuron is in various functional state and it is difficult for three-dimensional architecture of neuronal organelles to regulate in a simple rule.
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