| Project/Area Number |
02670027
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
神経解剖学
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| Research Institution | Okayama University |
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Principal Investigator |
NOMURA Takako Okayama University Medical School Department of Anatomy, Assistant, 医学部, 助手 (20116437)
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| Co-Investigator(Kenkyū-buntansha) |
MIZUKAWA Kiminao Okayama Univetsity Medical School, Department of Anatomy, Lecturer, 医学部, 講師 (40137154)
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| Project Period (FY) |
1990 – 1991
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| Project Status |
Completed (Fiscal Year 1991)
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| Budget Amount *help |
¥2,200,000 (Direct Cost: ¥2,200,000)
Fiscal Year 1991: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 1990: ¥1,400,000 (Direct Cost: ¥1,400,000)
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| Keywords | Receptor / Autoradiography / Muscarinic Cholinergic Receptor / Hemi-parkinsonian Rats / Dopamine receptor / Ischemia / GABAReceptors / 水迷路減試験 / 水迷路試験 / ヘミ・パ-キンソン病モデル / ド-パミン レセプタ- |
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| Research Abstract |
We established the procedures of receptor in vitro macro autoradiographical methods for neurotransmitter system in the central nervous system. By using these methods, we investigated the hemi-parkinsonian rats which were induced by the administration of 6-OHDA in the left substantia nigra. In the stratum of the hemi-parkisonian rats, dopaminergic receptors showed the up-regulation and dopamine uptake sites were completely disappeared. On the other hand, we could not find out the alteration of the cholinergic receptors concentration.
Administration of ibotenic acid to the Meynert nucleus, we induced expeimental dementia rats. In these rats, the cholinergic receptors in the cerebral cotex were disapperared.
The experimental ischemic rats were made by occulusion of the mid cerebral artery for 1 hour. We investigated the GABAnergic receptors in the ischemic rats brain using as ligands, 3H-labeled muscimol and SR 95531, which are considered much more specific binding ligand for GABA A receptor. As a results, GABAnergic receptors in the ischemic regions were significantly and simutanenously decreased.
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