Expression of period repeat sequence consisting in Drosopila clock gene in the rat brain : Insitu hybridization study.
| Project/Area Number |
02670028
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
神経解剖学
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| Research Institution | Okayama University |
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Principal Investigator |
TOKUMAGA Akira Okayama Univ. Medical School, Third Dept. of Anatomy. Professor, 医学部, 教授 (40009634)
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| Co-Investigator(Kenkyū-buntansha) |
ONO Katsuhiko Okayama Univ. Medical School. Third Dept. of Anatomy. Instructor, 医学部, 助手 (30152523)
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| Project Period (FY) |
1990 – 1991
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| Project Status |
Completed (Fiscal Year 1991)
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| Budget Amount *help |
¥400,000 (Direct Cost: ¥400,000)
Fiscal Year 1991: ¥400,000 (Direct Cost: ¥400,000)
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| Keywords | Clock Gene / Suprachiasmatic Nucleus / In Situ Hybridization / Circadian Rhythm / Rat Brain |
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| Research Abstract |
The clock gene of Drosophila containing a hexamer repeat sequence(ACAGGC)_n (period, per, repeat)is reported to be concerned in ther circadian rhythm. Young and his co-workers(1987)succeeded to clone a mouse genomic DNA containing the per repeat(cp2.2). In this study, temporal and spacial expression of CP2.2F and RB15 was examined in the adult rat brain by in situ hybridization. The former is a family of genes detected by using cp2.2 as a probe, and the latter is a gene corresponding to pRB15, one of the 11 cDNA recombinants homologous to cp2.2 from the rat brain cDNA libraly. Both genes were expressed in almost all neurons. However, it was in the suprachiasmatic nucleus that fluctuation to the genes under the light-dark cycle was apparently observed ; an intensive hybridization signal in the middle of the day and a weak in the middle of the night(p<0.01). On the other hand, the signal stayed relatively constant in other brain regions. A weak expression of both genes(CP2.2F and RB15)was detected in some glial and ependymal cells in the day but only a few in the night.
The present findings suggest that CP2.2F and RB15 may contribute to the circadian rhythm in the rat nervous system.
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Report
(3 results)
Research Products
(9 results)
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[Publications] Ishida, N., Noji, S., Ono, K., Koyama, E., Nohno, T., Taniguchi, S., Tokunaga, A., Fujii, T. and Mitsui, Y: "Diurnal regulation of per repeat mRNA in the suprachiasmatic nucleus in rat brain." Neuroschi. Letters. 122. 113-116 (1991)
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Related Report
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[Publications] Shibata, N., Noji, S., Ishida, M., Ono, K., Taniguchi, S., Mitsui, Y., Ohmoto, T., and Tokunaga, A.: "Expression of period repeat mRNA in the suprachiasmatic nucleus of the rat brain." Neurosci. Research. Suppl. 16. S152 (1991)
Description
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Related Report
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