| Project/Area Number |
02670147
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
Experimental pathology
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| Research Institution | Tokyo Women's Medical College |
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Principal Investigator |
KOBAYASHI Makio Tokyo Women's Medical College, Pathology, Prof., 医学部, 教授 (80060086)
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| Co-Investigator(Kenkyū-buntansha) |
SAWADA Tatsuo Nihon Univ,Sch.of Med, Pathology, Asso. Prof., 医学部, 助教授 (90162544)
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| Project Period (FY) |
1990 – 1992
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| Project Status |
Completed (Fiscal Year 1992)
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| Budget Amount *help |
¥2,200,000 (Direct Cost: ¥2,200,000)
Fiscal Year 1992: ¥400,000 (Direct Cost: ¥400,000)
Fiscal Year 1991: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 1990: ¥1,100,000 (Direct Cost: ¥1,100,000)
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| Keywords | retinoblastoma / adenovirus type 12 / DNA analysis / RB gene / ElA oncoprotein / southern blot analysis / in situ hybridization / Rb遺伝子 / in situハイブリダイゼ-ション / Rhodopsin |
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| Research Abstract |
To elucidate the early genetic change in tumorigensis of retinoblastoma, we investigated the genomic expression in retinal tumors induced by human adenovirus type 12 in rats, using various DNA probes. Twenty two rats received a single intraocular inoculation of concentrated virus fluid within 24 hours after birth, Intravitreous tumors were induced in one out of 22 animals ithin 118 days after the inoculation. The adenovirus related oncoprotein gene ElA and human retinoblastoma susceptibility gene H3-8 were detected in the tumors by southern blot hybridization. In sitt hybridization analysis demonstrated expression of adenovirus type 12 ElA gene in the inner granular layer of the retina within 3 hours after the inoculation. After then, N-myc gradually increased activity in transformed cells. It was suggested that integration of adenovirus type 12 ElA fragment with the host genome and expression of the gene following amplified N-myc were required for progression of this tumor.
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