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The tissue distribution of collagen-binding heat shock protein, HSP47, in fibrotic diseases.

Research Project

Project/Area Number 02670148
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field Experimental pathology
Research InstitutionNagoya University

Principal Investigator

SAGA Shinsuke  Nagoya University, School of Medicine, associate professor, 医学部, 助教授 (40144141)

Project Period (FY) 1990 – 1991
Project Status Completed (Fiscal Year 1991)
Budget Amount *help
¥2,300,000 (Direct Cost: ¥2,300,000)
Fiscal Year 1991: ¥1,200,000 (Direct Cost: ¥1,200,000)
Fiscal Year 1990: ¥1,100,000 (Direct Cost: ¥1,100,000)
KeywordsHeat shock protein / Collagen / Collagen-binding / Antibody / Fibrotic diseases
Research Abstract

Heat shock protein of molecular weight 47, 000 D, HSP47, binds to native and denatured collagen including type I, type III, or type IV. HSP47 is supposed to be implicated in the transportation of procollagen molecules from ER to Golgi. In order to determine immunohistochemically the distribution of HSP47 in liver or lung from the patients with fibrotic diseases, polyclonal and monoclonal antibodies against chicken HSP47 were used. Howerer, in the fixed mammalian tissues such antidobies were not able to atain HSP47 because of the disruption of the epitopes by fixation. Therefore, in order to get the antibody that react to human HSP47 even in the fixed tissues, human HSP47 was partially purified from NP-40 extract of human embryonal fibroblasts cultured in at least 10 roller bottles by gelatin affinity chromatography or immunoaffinity chromatography. It was very difficult to get further purified HSP47 because human HSP47 in the partially purified fraction was extremely unstable and easily degraded. This difficulty disturbed the production of the specific polyclonal or monoclonal antibody against human HSP47. Thus, immunohistochemical study for the distribution of HSP47 in the fibrotic diseases was not proceeded.
The trial of in situ hybridization with cDNA probe of mouse HSP47 in the section of liver or lung tissue of the fibrotic diseases failed to determine the distribution of mRNA for HSP47.

Report

(3 results)
  • 1991 Annual Research Report   Final Research Report Summary
  • 1990 Annual Research Report
  • Research Products

    (5 results)

All Other

All Publications (5 results)

  • [Publications] Miyaishi,O.: "Distribution of the collagen-binding heat shock protein in chicken tissues." J.Histochem.Cytochem.

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Miyaishi, O.: "Distribution of the collagen-binding heat shock protein in chicken tissues." J. Histochem. Cytochem.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Miyaishi,O.: "Distribution of the collagenーbinding heat shok protein in chicken tissues." J.Histochem.Cytochem.

    • Related Report
      1991 Annual Research Report
  • [Publications] Yokoi,T.: "Characterization of cell fusion in XC cells induced by Suncus murinus mammary tumor virus." Arch.Virol.115. 267-276 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] Nishizawa,K.: "Specific localization of phosphoーintermedate filament protein in the constricted area of dividing cells." J.Biol.Chem.

    • Related Report
      1990 Annual Research Report

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Published: 1990-04-01   Modified: 2016-04-21  

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