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Morpho-Physiological Study on Mechanism of Arrhythmia in Heart Muscle Cell by Using High-Speed Image Processor

Research Project

Project/Area Number 02670159
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field Experimental pathology
Research InstitutionKyoto Prefectural University of Medicine

Principal Investigator

TAKAMATSU Tetsuro  Kyoto Pref. Univ. Med. Dept. Path. Associate Prof., 医学部, 助教授 (40154900)

Project Period (FY) 1990 – 1991
Project Status Completed (Fiscal Year 1991)
Budget Amount *help
¥2,200,000 (Direct Cost: ¥2,200,000)
Fiscal Year 1991: ¥1,200,000 (Direct Cost: ¥1,200,000)
Fiscal Year 1990: ¥1,000,000 (Direct Cost: ¥1,000,000)
KeywordsArrhythmia / Heart Muscle Cell / Calcium Wave / Confocal laser Scanning Microscope / Image Processing / Indoー1
Research Abstract

I have developed a dual, digital fluorescence imaging system and new confocal laser scanning microscope employing an inverted optical system for imaging fast dynamic changes of the intracellular calcium ion concentration ([Ca^<2+>]_i) in heart muscle cells.
In 1990, Images of[Ca^<2+>]_i taken at 67-ms intervals during propagation of calcium waves revealed that the calcium wave front was constant in shape, spatially steep, typically rising from 500 to 1200 nM in about 10 mu m, and propagating at constant velocity, typically 100 mu m/sec at 22 ゚C. In 1991, I have investigate the biological application of a confocal laser scanning microscope for imaging the calcium wave. This instrument has several advantages for observing moving objects and flexible choice of scanning modes. 1) A faster scanning speed of up to 0.25 sec per frame including line scan, fast horizontal and vertical full-frame scan and a faster horizontal narrow band scan. 2) An efficient optical path specially designed for th … More e inverted fluorescence microscope system. 3) Direct transfer of digitized images from the frame memory in the CLSM to that of an image processor, avoiding the degradation of densitometric data by duplicated A/D and D/A conversion. These features gave us the opportunity to investigate the properties of the calcium wave during its propagation between isolated guinea-pig ventricular cell pairs. The actual[Ca^<2+>]_i is obtained directly through the fluorescence intensity of injected fluo-3, which responds to changes of[Ca^<2+>]_i in optically sectioned unit volumes of the cell. Optical sectioning images obtained enable us to determine the cell-to-cell junction between heart muscle paired cells and reveal that the high[Ca^<2+>]_i can evoke calcium-induced calcium release from the sarcoplasmic reticulum in the neighboring heart muscle cells through the gap junction without delay.
The confocal laser scanning microscope with depth-discriminating ability is a valuable tool for taking pictures of the sequence of biological events in living heart muscle cells such as the calcium wave, a model for arrhythmia at cellular level. Less

Report

(3 results)
  • 1991 Annual Research Report   Final Research Report Summary
  • 1990 Annual Research Report
  • Research Products

    (25 results)

All Other

All Publications (25 results)

  • [Publications] Tetsuro Takamatsu,Tetsuhiro Minamikawa,Hideyuki Kawachi and Setsuya Fujita: "Imaging of Calcium Wave Propagation in Guinea-Pig Ventricular Cell Pairs by Confocal Laser Scanning Microscopy" CELL STRUCTURE AND FUNCTION. 16. 341-346 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Tetsuhiro Minamikasa,Tetsuro Takamatsu and Setsuya Fujita: "Application of Laser Microtomography to in Situ Three-Dimensional Subcellular Morphology" ACTA HISTOCHEM.CYTOCHEM.24. 55-60 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Tetsuhiro Minamikasa,Tetsuro Takamatsu and Setsuya Fujita: "Laser Microtomography-3D Presentation and High-Temporal Resolution Imaging" ACTA HISTOCHEM.CYTOCHEM.24. 349-352 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] 高松 哲郎,南川 哲寛,藤田 晢也: "細胞の動的断層像がとれる共焦点レ-ザ高速走査顕微鏡" 組織培養. 16. 430-433 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] 南川 哲寛,高松 哲郎,河内 秀幸,藤田 晢也: "共焦点レ-ザ走査顕微鏡を用いた単離心筋細胞のカルシウム波の解析法" 医学のあゆみ. 155. 473-474 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] 河内 秀幸,南川 哲寛,高松 哲郎,藤田 晢也: "高速走査共焦点レ-ザ顕微鏡による心室筋対細胞間のカルシウム波伝播の解析" 心筋の構造と代謝. 13. 63-67 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] 藤田 晢也,高松 哲郎,南川 哲寛: "限界を超える生物顕微鏡(宝谷 紘一・木下 一彦編) 4章 立体断面を見る(共焦点レ-ザ-走査蛍光顕微鏡)" 学会出版センタ-, 177 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] 高松 哲郎,南川 哲寛,藤田 晢也: "組織細胞化学 レ-ザ顕微鏡の原理,技術と医学生物学への応用" 学際企画, 196 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Minamikawa, T., Takamatsu, T., Kawachi, H. and Fujita, S: "Calcium waves in isolated cardiac myocytes as revealed by confocal laser scanning microscope" Igaku no ayumi. 155. 473-474 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Takamatsu, T., Minamikawa, T. and Fujita, S: "High-velocity microscopic tomography on the living cell" Soshiki Baiyou. 16. 430-433 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Minamikawa, T., Takamatsu, T., and Fujita, S.: "Application of laser microtomography to in situ three-dimensional subcellular morphology." Acta Histochem. Cytochem.24. 55-60 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Minamikawa, T., Takamatsu, T. and Fujita, S.: "Laser microtomography -3D presentation and high-temporal resolution imaging." Acta Histochem. Cytochem.24. 349-352 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Takamatsu, T., Minamikawa, T., Kawachi, H. and Fujita, S: "Imaging of calcium wave propagation in guinea-pig ventricular cell pairs by confocal laser scanning microscopy" Cell Struct. Func.16. 341-346 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Takamatsu, T., Minamikawa, T. and Fujita, S: "Confocal laser scanning microscope and its application to biology and medicine" Histochemistry and cytochemistry. 1991. 171-181 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Fujita, S, Minamikawa, T. and Takamatsu, T.: "Confocal laser scanning microscope" Spectroscopical Society of Japan. Series 21. 53-69 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Tetsuro Takamatsu,Tetsuhiro Minamikawa,Hideyuki Kawachi and Setsuya Fujita: "Imaging of Calcium Wave Propagation in GuineaーPig Ventricular Cell Pairs by Confocal Laser Scanning Microscopy" CELL STRUCTURE AND FUNCTION. 16. 341-346 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] Tetsuhiro Minamikawa,Tetsuro Takamatsu and Setsuya Fujita: "Application of Laser Microtomography to in Situ ThreeーDimensional Subcellular Morphology" ACTA HISTOCHEM.CYTOCHEM.24. 55-60 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] Tetsuhiro Minamikawa,Tetsuro Takamatsu and Setsuya Fujita: "Laser Microtomographyー3D Presentation and HighーTemporal Resolution Imaging" ACTA HISTOCHEM.CYTOCHEM.24. 349-352 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] 高松 哲郎,南川 哲寛,藤田 晢也: "細胞の動的断層像がとれる共焦点レ-ザ高速走査顕微鏡" 組織培養. 16. 430-433 (1990)

    • Related Report
      1991 Annual Research Report
  • [Publications] 南川 哲寛,高松 哲郎,河内 秀幸,藤田 晢也: "共焦点レ-ザ走査顕微鏡を用いた単離心筋細胞のカルシウム波の解析法" 医学のあゆみ. 155. 473-474 (1990)

    • Related Report
      1991 Annual Research Report
  • [Publications] 河内 秀幸,南川 哲寛,高松 哲郎,藤田 晢也: "高速走査共焦点レ-ザ顕微鏡による心室筋対細胞間のカルシウム波伝播の解析" 心筋の構造と代謝. 13. 63-67 (1990)

    • Related Report
      1991 Annual Research Report
  • [Publications] 藤田 晢也,高松 哲郎,南川 哲寛: "限界を超える生物顕微鏡(宝谷 紘一,木下 一彦編)4章立体断面を見る(共焦点レ-ザ-走査蛍光顕微鏡)" 学会出版センタ-, 177 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] 高松 哲郎,南川 哲寛,藤田 晢也: "組織細胞化学 レ-ザ顕微鏡の原理,技術と医学生物学への応用" 学際企画, 196 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] 高松 哲郎: "細胞の動的断層像がとれる共焦点レ-ザ高速走査顕微鏡" 組織培養. 16. 430-433 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] 高松 哲郎: "共焦点レ-ザ走査顕微鏡を用いた単離心筋細胞のカルシウム波の解析法" 医学のあゆみ. 155. 473-474 (1990)

    • Related Report
      1990 Annual Research Report

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Published: 1990-04-01   Modified: 2016-04-21  

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