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Analysis of polysaccharide in the cyst wall of Pneumocystis carinii and therapeutic strategy for pneumocystosis using beta-1, 3-glucan biosynthesis inhibitor in vivo.

Research Project

Project/Area Number 02670171
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field 寄生虫学(含医用動物学)
Research InstitutionKyoto Prefectural University of Medicine

Principal Investigator

YAMADA Minoru  Kyoto Prefectural University of Medicine, Department of Medical Zoology, Lecturer, 医学部, 講師 (70106392)

Co-Investigator(Kenkyū-buntansha) MATSUDA Shinji  Kyoto Prefectural University of Medicine, Department of Medical Zoology, Instruc, 医学部, 助手 (70199800)
YOSHITSUGU Matsumoto Kyo  of Medicine, Department of Medical Zoology, Instructor, 医学部, 助手 (00173922)
Project Period (FY) 1990 – 1991
Project Status Completed (Fiscal Year 1991)
Budget Amount *help
¥2,300,000 (Direct Cost: ¥2,300,000)
Fiscal Year 1991: ¥600,000 (Direct Cost: ¥600,000)
Fiscal Year 1990: ¥1,700,000 (Direct Cost: ¥1,700,000)
KeywordsPneumocystis carinii / pellicle / beta-1, 3-glucan biosynthesis inhibitor / Therapy / Prophylaxis / Aculeacin A / Glucan / Aculeacin A and trimethoprim-sulfamethoxazole / Zymolyase
Research Abstract

In vivo activities of a beta-1, 3-glucan biosynthesis inhibitor, aculeacin A, against P. carinii and P. carinii pneumonia were investigated using the rat model. Intraperitoneal administration of aculeacin A at 25 mg/kg or 10 mg/kg, or even at 2 mg/kg per day for 2 weeks from 5 Weeks of steroid treatment, affected cyst wall formation, inhibited cyst maturation, and prevented severe pneumonia in steroid-treated rats. Administration of aculeacin A at 10 mg/kg, or even at 2 mg/kg per day earlier, during the initial phase of infection, was more effective suggesting that this drug can be used prophilactically. Simultaneous administration of aculeacin A (0.4 mg/kg) and trimethoprim-sulfamethoxazole (1mg/kg as a dose of sulfamethoxazole) from 5 weeks of steroid treatment showed therapeutic effects against rat pneumocystosis. This data suggested that dosages of both drugs were reduced, though distinct synergy of use of both drugs was not given in this case.
Thus it is hoped that studies of beta-1, 3-glucan biosynthetic inhibitors will not only help in the understanding of the biochemical pathways of the organisms, but also lead to improved human pneumocystosis chemotherapy.

Report

(3 results)
  • 1991 Annual Research Report   Final Research Report Summary
  • 1990 Annual Research Report
  • Research Products

    (17 results)

All Other

All Publications (17 results)

  • [Publications] Minoru Yamada: "Activity of Aculeasin A,an inhibitor of β-1,3-glucan synthesis,against Phneumocystis carinii pneumonia" Bulletin de la Societe Francaise Parasitologie. 1074 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Yoshitsugu Matsumoto: "Yeast glucan of Pneumocystis carinii cyst wall:An excellent target for chemotherapy" Journal of Protozoology. 38. 6S-7S (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] 山田 稔: "ニュ-モシスチス・カリニ肺炎の治療薬としてのグルカン合成阻害薬,aculeacin A" 寄生虫学雑誌. 40. 200 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] 松本 芳嗣: "グルカン合成阻害薬,aculeacin Aによるニュ-モシスチス・カリニ嚢子壁の形成阻害" 寄生虫学雑誌. 40. 200 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] 松本 芳嗣: "グルカン合成阻害薬,aculeacin Aによるニュ-モシスチス・カリニ肺炎予防効果とその作用機作に関する考察" 寄生虫学雑誌(発表予定). 41. (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] 松本 芳嗣: "グルカン合成阻害薬aculeacin AとST合剤の併用による実験的ラット・ニュ-モシスチスカリニ肺炎の予防" 寄生虫学雑誌. 41. 116 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] M. Yamada: "Activity of aculeacin A, an inhibitor of beta-1, 3-glucan synthesis, against Pneumocystis carinii pneumonia" Bulletin de la Societe Francaise de Parasitologie TOME 8/1990 Supplt n 2/Resumes-Abstracts. 1074

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Y. Matsumoto: "Yeast glucan of Pneumocystis carinii cyst wall : An excellent target for chemotherapy" Journal of Protozoology. 38(6). 6S-7S

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] 松本 芳嗣: "グルカン合成阻害薬,aculeacinAによるニュ-モシスチス・カリニ嚢子壁の形成阻害" 寄生虫学雑誌. 40. 200 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] 山田 稔: "ニュ-モシスチス・カリニ肺炎の治療薬としてのグルカン合成阻害薬,aculeacinA" 寄生虫学雑誌. 40. 200 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] Yoshitsugu Matsumoto: "Yeast glucan of Pneumocystis carinii cyst wall;An excellent target for chemotherapy" Journal of Protozoology. 38. 6S-7S (1992)

    • Related Report
      1991 Annual Research Report
  • [Publications] 松本 芳嗣: "グルカン合成阻害薬,aculeacinAによるニュ-モシスチス・カリニ肺炎予防効果とその作用機作に関する考察" 寄生虫学雑誌. 41. (1992)

    • Related Report
      1991 Annual Research Report
  • [Publications] 松本 芳嗣: "グルカン合成阻害薬aculeacinAとST合剤の併用による実験的ラット・ニュ-モシスチスカリニ肺炎の予防" 寄生虫学雑誌. 41. 116 (1992)

    • Related Report
      1991 Annual Research Report
  • [Publications] Minoru Yamada: "Activity of aculeacin A,an inhibitor of βー1,3ーglucan synthesis,against Pneumocystis carinii pneumonia" Bulletin de la Societe Francaise de Parasitologie. 1074 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] Yoshitsugu Matsumoto: "Yeast glucan of Pneumocystis carinii cyst wall as a new target for therapy"

    • Related Report
      1990 Annual Research Report
  • [Publications] 松本 芳嗣: "グルカン合成阻害薬,aculeacinAによるニュ-モシスチス・カリニ嚢子壁の形成阻害" 寄生虫学雑誌. (1991)

    • Related Report
      1990 Annual Research Report
  • [Publications] 山田 稔: "ニュ-モシスチス・カリニ肺炎の治療薬としてのグルカン合成阻害薬,aculeacinA" 寄生虫学雑誌. (1991)

    • Related Report
      1990 Annual Research Report

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Published: 1990-04-01   Modified: 2016-04-21  

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