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Mechanism of heat resistance of bacterial spores

Research Project

Project/Area Number 02670185
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field 細菌学
Research InstitutionNagoya City University

Principal Investigator

TOCHIKUBO Kunio  Nagoya City University Medical School, Professor, 医学部, 教授 (30079991)

Co-Investigator(Kenkyū-buntansha) ICHIKAWA Yuko  Nagoya City University Medical School, Research Associate, 医学部, 助手 (80173441)
YASUDA Yoko  Nagoya City University Medical School, Assistant Professor, 医学部, 講師 (70080009)
Project Period (FY) 1990 – 1991
Project Status Completed (Fiscal Year 1991)
Budget Amount *help
¥2,300,000 (Direct Cost: ¥2,300,000)
Fiscal Year 1991: ¥600,000 (Direct Cost: ¥600,000)
Fiscal Year 1990: ¥1,700,000 (Direct Cost: ¥1,700,000)
KeywordsBacillus subtilis / Bacterial spore / Heat resistance / Hydrostatic pressure / Germination / Dipicolinic acid / Glucose dehydrogenase / 枯草菌芽胞 / 高圧
Research Abstract

The following experiments were carried out for the purpose of clarification of the heat resistance of bacterial spores.
1. Death of bacterial spores by hydrostatic pressure at various temperatures.
The dormant spores of Bacillus subtilis treated at 4゚C for 1 h with hydrostatic pressures between 1, 000 and 5, 000 kg/cm^2 remained alive without being affected very much. On the other hand, a great majority of dormant spores treated at 37゚C and 60゚C under the same conditions of hydrostatic pressure were killed and the phenomena observed resembled those of physiological germination by L-alanine.
2. Purification of inactive glucose dehydrogenase from dormant spores.
Glucose dehydrogenase is considered to play an important role in energy production immediately after germination and we have estimated that the enzyme is present in dormant spores as an inactive form consisting of two subunits with M. W. of 30K and becomes an active form having four subunits during germination. Therefore, purification of the inactive form of the enzyme from B. subtilis spores has been tried using the antibodies raised against the 192-207 peptide and the active fore of the enzyme from Bacillus megaterium. The latter antibody appeared to be available. The binding of dipicolinic acid (DPA) to the purified inactive enzyme would be examined in connection with heat resistance of spores.
3. Detection and purification of DPA-binding macromoleculer substances in dormant spores.
Although DPA is contained abundantly in bacterial spores, its role remains unclear. Therefore, we have been trying to detect some DPA-binding macromolecular substances in dormant spores of B. subtilis using the antibody against DPA. Two substances with H. W. of 440K and 230K were detected and their purification is in progress. The role of DPA in heat resistance would be examined.

Report

(3 results)
  • 1991 Annual Research Report   Final Research Report Summary
  • 1990 Annual Research Report
  • Research Products

    (11 results)

All Other

All Publications (11 results)

  • [Publications] Yoko Nakanishi,Yoko Yasuda,Yuko Ichikawa,Kunio Tochikubo: "Lethal effects of different pressures and temperatures on Bacillus subtilis spores" Journal of General Microbiology. 138. (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Shigeko Nishioka,Yoko Yasuda,Kunio Tochikubo: "Purification of an inactive form of glucose dehydrogenase from Bacillus subtilis spores" Microbiology and Immunology. 36. (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Yutaka Matano,Yoko Yasuda,Kunio Tochikubo: "Detection of dipicolinic acid-binding macromolecules in dormant spores" FEMS Microbiology Letters. 90. (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Yoko Nakanishi, Yoko Yasuda, Yuko Ichikawa and Kunio Tochikubo: "Lethal effects of different pressures and temperatures on Bacillus subtilis spores" Journal of General Microbiology. 138. (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Shigeko Nishioka, Yoko Yasuda and Kunio Tochikubo: "Purification of an inactive form of glucose dehydrogenase from Bacillus subtilis spores" Microbiology and Immunology. 36. (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Yutaka Matano, Yoko Yasuda and Kunio Tochikubo: "Detection of dipicolinic acid-binding macromolecules in dormant spores" FEMS Microbiology Letters. 90. (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Yoko Nakanishi、Yoko Yasuda、Yuko Ichikawa、Kunio Tochikubo: "Lethal effects of different pressures and temperatures on Bacillus subtilis spores" Journal of General Microbiology. 138. (1992)

    • Related Report
      1991 Annual Research Report
  • [Publications] Shigeko Nishioka、Yoko Yasuda、Kunio Tochikubo: "Purification of an inactive form of glucose dehydrogenase from Bacillus subtilis spores" Microbiology and Immunology. 36. (1992)

    • Related Report
      1991 Annual Research Report
  • [Publications] Yutaka Matano、Yoko Yasuda、Kunio Tochikubo: "Detection of dipicolinic acidーbinding macromolecules in dormant spores" FEMS Microbiology Letters. 90. (1992)

    • Related Report
      1991 Annual Research Report
  • [Publications] Kunio Tochikubo, Yoko Yasuda, Yuko Ichikawa, Yoko Nakanishi: "Lethal effects of different pressures and temperatures on Bacillus subtilis spores" Journal of General Microbiology. 138. (1992)

    • Related Report
      1990 Annual Research Report
  • [Publications] Kunio Tochikubo, Yoko Yasuda, Shigeko Nishioka: "Purification of an inactive form of glucose dehydrogenase from Bacillus subtilis spores" Microbiology and Immunology. 36. (1992)

    • Related Report
      1990 Annual Research Report

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Published: 1990-04-01   Modified: 2016-04-21  

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