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Molecular Biology and Morphology of Cell Membranes Modified by Infection with Herpes Simplex virus

Research Project

Project/Area Number 02670194
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field Virology
Research InstitutionOkayama University

Principal Investigator

NII Shiro  Okayama University Medical School, Department of Virology, Professor, 医学部, 教授 (40029757)

Co-Investigator(Kenkyū-buntansha) ARAO Yujiro  Okayama University Medical School, Department of Virology, Research Associate (, 医学部, 助手 (40151146)
YOSHIDA Mariko  Okayama University Medical School, Department of Virology, Research Associate, 医学部, 助手 (20144743)
YAMADA Masao  Okayama University Medical School, Department of Virology, Assistant Professor, 医学部, 講師 (40166731)
Project Period (FY) 1990 – 1991
Project Status Completed (Fiscal Year 1991)
Budget Amount *help
¥2,000,000 (Direct Cost: ¥2,000,000)
Fiscal Year 1991: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 1990: ¥1,000,000 (Direct Cost: ¥1,000,000)
KeywordsHSV-1 / Hemadsorption / Human Herpesvirus 6 / Fc receptor / Microvilli / Tegument / テグメント / hemadsorption / 糖蛋白 / microvilli
Research Abstract

Experiments were conducted principally on two subjects along the above described project and the following results were obtained.
(1)Adsorption of sensitized sheep erythrocytes to FL cells infected with HSV-1 was studied biologically and morphologically. Almost 100% of the FL cells at a late stage of infection(i. e. 23 hrs)were positive for the appearance of HSV-1 gC antigen, while about 86% of them had hemadsorption(HAD)activity. To elucidate why all the infected cells do not show HAD activity, FL cells at the similar stage of infection were examined by high-resolution scanning electron microscopy and also by immuno-scanning electron microscopy. When infected cells were treated first with peroxidaseantiperoxidase(PAP)rabbit IgG and then with goat anti-rabbit IgG labeled with colloidal gold, the surface of all the cells were clearly tagged with colloidal gold particles. Morphological observations of HAD revealed that many microvilli adhered to the surface of erythrocytes. Thus, in addition to the expression of HSV-induced FcR on the surface membrane of infected cells, which is prerequisite for HAD, microvilli play an essential role on the appearance of this phenomenon.
(2)Human lymphocytes and MT4 cells infected with human herpesvirus 6(HHV-6)were examined electron microscopically, with special attention to the interaction of viral particles with cellular membranes. While the distinct tegument coating the capsids was not revealed in enveloped viral particles in the perinuclear cisternae or around intranuclear viral particles, it was clearly demonstrated for all the capsids located in the cytoplasm as well as extracellularly. This finding provides the basis for proposing a possible viral transport mechanism, i. e. the egress of HHV-6 is achieved by fusion of the envelope of a perinuclear virus with the outer lamella of the nuclear membrane and reenvelopment of the resulting cytoplasmic nucleocapsid at a cytoplasmic membrane.

Report

(3 results)
  • 1991 Annual Research Report   Final Research Report Summary
  • 1990 Annual Research Report
  • Research Products

    (25 results)

All Other

All Publications (25 results)

  • [Publications] 白 増亮: "単純ヘルペスウイルス感染細胞表面に発現するFcーレセプタ-による感作赤血球吸着反応:微細形態学的ならびに細胞生物学的考察" 岡山医学会雑誌. 103. 267-281 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] 新居 志郎: "ヒトヘルペスウイルス6の成熟と放出過程" 電子顕微鏡. 27. (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Z.L.Bai: "Electron Microscopic Study of Hemadsorption Phenomenon in Cultured Cells Caused by HSV-1 Induced Fc-Receptor" Journal of Electron Microscopy. 43. (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] S.Nii: "Maturation and Release of Herperviruses" Journal of Electron Microscopy. 43. (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] S.Nii: "Immunobiology and Prophylaxis of Human Herpesvirus Infections" Plenum Press, 10 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] M.Yoshida: "Electro Microscopy 1990,Vol.3 (Proceedoings of the XII the International Congress for Electron Microscopy)" San Francisco Press, 2 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] S.Nii: "World Health Organization Bi-regional Training Course on Electron Microscopy in Bio-Medical Research and Diagnosis of Human Diseases" Educational Technology Unit and Printing Section of the University of Adelatide, 11 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] 新居 志郎: "顕微鏡サイエンスの展望ー基礎と応用" 日本電子顕微鏡学会第36回シンポジウム実行委員会, 4 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] 新居 志郎: "SCAN TECH '92" 日本電子顕微鏡学会・SEM研究部会, 3 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Bai Z. L.: "Hemadsorption phenomenon in cultured cells caused by HSV-1 induced Fc receptor : ultrastructural and biological studies" Okayama Igakkai Zasshi. 103, No. 4. 267-281 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Nii S.: "Maturation and release of human herpesvirus 6 (In Japanese)" Electron-Microscopy. 27. (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Nii S. (et al.): "Matruration and release of herpesviruses" J Electron Microsc. 42. (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Bai Z. l. (et al.): "Electron microscopic study of hemadsorption phenomenon in cultured cells caused by HSV-1 induced Fc receptor" J Electron Micros. 43. (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Nii S. (et al.): "Replication of human herpesvirus 6(HHV-6) : morphological aspects" Immunobiology and prophylaxis of human herpesvirus infections. Plenum Press, New York. 19-28 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Yoshida M. (et al.): "Electron microscopic study of human herpesvirus 6 (HHV-6)" Electron Microscopy 1990,. 3, Proceedings of the twelfth international congress on electron microscopy, San Francisco Press, Inc.,. 384-385 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Nii S (et al.): "Transmission and scanning electron microscopy of human herpesviuses" World health organization bi-regional training course on electron microscopy in biomedical research and diagnosis of human diseases. The Educational Technology Unit and The Printing Section of The University of Adelaide. 186-196 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Nii S.: "Electron microscopic study on replication of herpesviruses" Microscopic science : basis and application, Proceedings of the thirty-sixth sympodium on electron microscopy. 53-56 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Nii S: "Scanning electron microscopy of viruses and infected cells" SCAN TECH '92, Research Group for Scanning Electron Microscopy. 59-61 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] 新居 志郎: "ヒトヘルペスウイルス6の成熟と放出過程" 電子顕微鏡. 27. (1992)

    • Related Report
      1991 Annual Research Report
  • [Publications] Z.L.Bai: "Electron Microscopic study of Hemadsorption Phenomenon in Cultured Cells Caused by HSVー1 Induced Fc Receptor" Journal of Electron Microscopy. 43. (1993)

    • Related Report
      1991 Annual Research Report
  • [Publications] S.Nii: "Maturation and Release of Herpesviruses" Journal of Electron Microscopy. 43. (1993)

    • Related Report
      1991 Annual Research Report
  • [Publications] S.Nii: "World Health Organization.BiーRegional Training Course on Electron Microscopy in BioーMedical Research and Diagnosis of Human Diseases" Educational Technology Unit and Printing Section of the University of Adelaide, 11 (1992)

    • Related Report
      1991 Annual Research Report
  • [Publications] 白 増亮: "単純ヘルペスウイルス感染細胞表面に発現するFcーレセプタ-による感作赤血球吸着反応:微細形態学的ならびに細胞生物学的考察" 岡山医学会雑誌. 103. (1991)

    • Related Report
      1990 Annual Research Report
  • [Publications] S.Nii: "Immunobiology and Prophylaxis of Human Herpesvirus Infections" Plenum Press, 10 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] M.Yoshida: "Electron Microscopy 1990" San Francisco Press, 2 (1990)

    • Related Report
      1990 Annual Research Report

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Published: 1990-04-01   Modified: 2016-04-21  

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