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Functions of the mutated NS1 protein of influenza B virus which has a long caroxyl terminal deletion.

Research Project

Project/Area Number 02670196
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field Virology
Research InstitutionJichi Medical School

Principal Investigator

TOBITA Kiyotake  Jichi Medical School, Professor, 医学部, 教授 (00077174)

Co-Investigator(Kenkyū-buntansha) ODAGIRI Takato  Jichi Medical School, Lecturer, 医学部, 講師 (80177237)
TANAKA Toshinori  Jichi Medical School, Lecturer, 医学部, 講師 (30146154)
TASHIRO Masato  Jichi Medical School, Assistant Professor, 医学部, 助教授 (90111343)
Project Period (FY) 1990 – 1991
Project Status Completed (Fiscal Year 1991)
Budget Amount *help
¥2,300,000 (Direct Cost: ¥2,300,000)
Fiscal Year 1991: ¥900,000 (Direct Cost: ¥900,000)
Fiscal Year 1990: ¥1,400,000 (Direct Cost: ¥1,400,000)
Keywordsinfluenza B virus / NS gene / nucleotide sequence / deletion mutation / NS1 protein / cytopathic effect / インフルエンザウイルス / 変異体 / RNA合成 / CPE
Research Abstract

By hetero typic crosses of influenza A and B viruses, we isolated 3 mutants of influenza virus B/Yamagata, which carried deletion mutations in their NS genes, namely, clone 201, AUB2-253 and AWBY-234. Due to a frame-shift downstream, all of them expressed greatly truncated NS1 protein which lacked more than half of the carboxyl terminal portion of the molecule, leaving NS2 intact. To delineate the functions of the NS gene of influenza virus unequivocally, we first prepared a reassortant virus between clone 201 and wild type B/Lee/40, in which only the mutated NS gene of clone 201 was inserted in place of that of B/Lee. As a control, we also prepared a. control reassortant between wild type, Lee/40 and wild type B/Yamagata, in which only the NS gene was substituted with the wild type NS gene of Yamagata. Comparative studies of the two reassortants revealed the following.
(1) A large carboxyl terminal deletion of the NSI protein of influenza virus greatly accelerates cell sestruction.
(2) Such a deletion mutation does not affect viral protein synthesis.
(3) Carboxyl terminal deletion of the NS1 protein alters the mode of VRNA synthesis.
The presence of a large amount of subgenomic RNAs within the virion and the occurrence of multiplicity reactivation suggested that a deletion in NS1 protein inhibited efficient elongation of the nascent viral RNA chains.

Report

(3 results)
  • 1991 Annual Research Report   Final Research Report Summary
  • 1990 Annual Research Report
  • Research Products

    (15 results)

All Other

All Publications (15 results)

  • [Publications] K.Tobita: "Nucleotide sequence and some biological properties of the NS gene of a newly isolated influenza B virus mutant which has a long carboxyterminal deletion in the NS1 protein." Virology. 174. 314-319 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] T.Odagiri: "Mutation in NS2,a nonstructural protein of influenza A Virus,extragenically causes aberrant replication and expression of the PA gene and leads to generation of defective interfering particles." Proceedings of the National Academy of Science of the United States of America. 87. 5988-5992 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] T.Odagiri: "Synthesis of the NS2 nonstructural protein messenger RNA of influenza viruses occurs in the absence of viral protein synthesis." Archives of Virology. 184. 227-234 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] M.Tashiro: "Pneumotropic revertants derived from a pantropic mutant,F1-R,of Sendai virus." Virology. 184. 227-234 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] K.Tobita: "Persistence of viral genes in a variant of MDBK cell after productive replication of a mutant of influenza virus A/WSN." Virology.

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] K. Tobita, T. Tanaka, T. Odagiri, M. Tashiro and S. -Y. Feng: "Nucleotide sequence and some biological properties of the NS gene of a newly-isolated influenza B virus mutant which has a long carboxyl terminal deletion in the NS1 protein." Virology. 174. 314-319 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] T. Odagiri and K. Tobita: "Mutation in NS2, a nonstructural protein of influenza A virus, extragenically causes aberrant replication and expression of the PA gene and leads to generation of defective interfering particles." proceedings of the National Academy of Science of the United States of America. 87. 5988-5992 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] T. Odagiri, K. Tobita and M. Tashiro: "Synthesis of the NS2 nonstructural protein messenger RNA of influenza viruses occurs in the absence of viral protein synthesis." Archives of Virology. 184. 227-234 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] M. Tashiro, I. James, S. Karri, K. Wahn, K. Tobita, H. -D. Klenk, R. Rott and J. Seto: "Pneumotropic revertants derived from a pantropic mutant, F1-R, of Sendai virus." Virology. 184. 227-234 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] K.Tobita: "Persistence of viral genes in a variant of MDBK cell after productive replication of a mutant of influenza virus A/WSN." Virology.

    • Related Report
      1991 Annual Research Report
  • [Publications] M.Tashiro: "Pneumotropic revertants derived from a pantropic mutant, F1ーR, of Sendai virus." Virology. 184. 227-234 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] T.Odagiri: "Synthesis of the NS2 nonstructural protein messenger RNA of influenza viruses occurs in the absence of viral protein synthsis." Archives of Virology. 120. 281-288 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] K.Tobita: "Nucleotide sequence and some biological properties of the NS gene of a newly isolated influenza B virus mutant which has a long carboxyl terminal deletion in the NS1 protein." Virology. 174. 214-219 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] T.Odagiri: "Mutation in NS2, a nonstructural protein of influenza A virus, extragenically causes aberrant replication and expression of the PA gene and leads to generation of DI particles." Proc.Nat.Acad.Sci.USA. 87. 5988-5992 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] M.Tashiro: "Altered budding site of a pantropic mutant of Sendai viru,F1ーR,in polarized epithelial cells." J.Virol.64. 4672-4677 (1990)

    • Related Report
      1990 Annual Research Report

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Published: 1990-04-01   Modified: 2016-04-21  

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