Proliferation of double negative (DN) gammadelta T cells in reseponse to allogeneic MHC antigen.
| Project/Area Number |
02670206
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
Immunology
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| Research Institution | Nagasaki University |
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Principal Investigator |
NAKAYAMA Eiichi Nagasaki University School of Medicine, Associate Professor., 医学部, 助教授 (60180428)
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| Co-Investigator(Kenkyū-buntansha) |
MIENO Masahiro Nagasaki University School of Medicine, Assistant Professor., 医学部, 助手 (60039576)
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| Project Period (FY) |
1990 – 1991
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| Project Status |
Completed (Fiscal Year 1992)
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| Budget Amount *help |
¥2,300,000 (Direct Cost: ¥2,300,000)
Fiscal Year 1991: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 1990: ¥1,600,000 (Direct Cost: ¥1,600,000)
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| Keywords | T-cells / Double negative / gammadelta / T細胞抗原レセプタ-(TCR) / γδT細胞 / T細胞機能 / TL抗原 / トランスジェニックマウス / T細胞抗原レセプタ-(αδ) / ダブル・ネガティブ細胞 |
|---|
| Research Abstract |
1. In TL transgenic mouse strain C3H.Tg.Tla^a-3.1, gammadelta T cells start to increase from three weeks after birth and reach to about 30% of total T cells in peripheral lymphoid tisene. It was demonstrated that gammadelta T cells in these mice respond to proliferate in stimulation with immobilized anti-TCR delta mAb.
2. Spleen cells from C3H.Tg.Tla^a-3.1 mice proliferated in response to mitomycin C (MMC) treated BALB/c stimulator cells. In these cultures, proliferative response was observed with gammadelta as well as alphabeta T cells. However, when alphabeta T cells were eliminated before MLC, no prolifenation was observed with gammadelta T cells. Thus, proliferative response of gammadelta T cells was dependent on alphabeta T cells. gammadelta T cells recovered after MLR culture showed no cytotoxicity against ^<51>Cr-labeled BALB/c target cells.
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Report
(3 results)
Research Products
(7 results)
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[Publications] Mieno, M., Suto, R., Obata, Y., Udono, H., Takahashi, T., Shiku, H., and Nakayama, E.: "CD4 CD8 T cell receptor alphabeta T cells: generation of an in vitro major histocompatibility complex class I specific cytotoxic T lymphocyte response and allogeneic tumor rejection." J. Exp. Med.174. 193-201 (1991)
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