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Studies on HRF 20, a novel membrane glycoprotein which restrict homologous complement reaction

Research Project

Project/Area Number 02670210
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field Immunology
Research InstitutionFukuoka University

Principal Investigator

OKADA Noriko  Fukuoka Univ. Sch. Med. Microbiol. Assistant Prof., 医学部, 助手 (20160682)

Project Period (FY) 1990 – 1991
Project Status Completed (Fiscal Year 1991)
Budget Amount *help
¥900,000 (Direct Cost: ¥900,000)
Fiscal Year 1991: ¥900,000 (Direct Cost: ¥900,000)
KeywordsComplement / HRF20 / Glycoprotein / PNH / HIV / AIDS / Mebrane / Monoclonal antibody / HRF
Research Abstract

We developed a monoclonal antibody, 1F5, which sensitizes human erythrocytes for hemolysis by homologous human complement. The antigenic molecule was identified to be HRF20 (20 kDa homologous restriction factor) which inhibit the reaction of C8 and C9 to form a membrane attack complex on C5b67, the trimolecular intermediate complement complex on cell membrane.
HRF20 was found to be deficient on the abnormal erythrocytes of patients suffering from paroxysmal nocturnal hemoglobinuria (PNH). Adsorption of purified HRF20 on to the abnormal PNH erythrocytes reconstituted their ability to resist against homologous complement attack. We encountered to find a patient who is hereditary-deficient in HRF20 expression. Analysis of HRF20 gene of the patient revealed that one base deletion was found at 2 sites of the patient's HRF20 gene.
Immunohistochemical analysis revealed that HRF20 is expressed not only on blood cells but also on endothelial cells, kidney cells, nerve Schwann cells, etc. HRF20 is also significantly expressed on fetal cells indicating that it would be playing a role to protect fetal, cells including nerve cell from membrane damage by complement.
We also found that HRF20 expression was decreased on CD8 bright T lymphocytes from HIV-infected patients even before appearance of AIDS or ARC symptoms.

Report

(3 results)
  • 1991 Annual Research Report   Final Research Report Summary
  • 1990 Annual Research Report
  • Research Products

    (31 results)

All Other

All Publications (31 results)

  • [Publications] A.Tomita N.Okada & H.Okada: "Comparative study of decay accelerating factor and HLA-DR within the CD8-bright population." Eur.J.Immunol.21. 1843-1848 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] M.Yamashina E.Ueda T.Kinoshita T.Tanami et.al: "Inherited complete dificiency of 20kDa homologous restriction factor (CD59) as a cause of paroxysmal nocturnal hemoglobinuria." New Engl.J.Med.323. 1184-1189 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] H.Okada N.Okada & T.Hideshima: "HRF 20,a membrane inhibitor of complement attack,does not protect cells from the cytotoxic reaction by lymphokine activated killer cells." Biochem.Biophys,Res,Comm.171. 717-721 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] R.Harada N.Okada T.Fijita & H.Okada: "Purification of 1F5 antigen which prevents complement attack on on homologous cell membranes." J.Immunology.144. 1823-1828 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] T.Hideshima N.Okada & H.Okada: "Expression of HRF 20,a regulatory molecule of complement activation,on perpheral blood mononuclear cells." Immunology.69. 396-401 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] N.Okada R.Harada & H.Okada: "Erythrocytes of paroxysmal nocturnal hemoglobinuria patients acqure resistance to complement attack by purified 20kDa homologous restriction factor (HRF20)." Clin.Exp.Immunology.80. 109-113 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] A.Tomita,N.Okada,R.Watanabe T.Shinohara,H.Toriya et.al: "Flontiers of mucosal immundogy Vol 1:Altered expression of DAF & HRF20 regulatory molecules of complement activation on peripheral bood mononuclear cells in Ulcerative colitis." Elsevier Sience Publishers., (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] R.Watanabe,A.Tomita N.Okada H.Toriya et,al: "Flontiers of mucosal immundogy Vol 1:Immunohistological study of Ulcerative colitis using monoclonal autibadies against HRF20 and DAF." Elsevier Sience Publishers., (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Okada, N., Harada, R. & Okada, H.: "Erythrocytes of paroxysmal nocturnal hemoglobinuria patients acquire resistance to complement attack by purified 20 kDa homologous restriction factor (HRF20)" Clin. Exp. Immunol.80. 109-113 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Harada, R., Okada, N. & Okada, H.: "Purification of 1F5 antigen which prevents complement attack on homologous cell membranes" J. Immunol. 144. 1823-1828 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Hideshima, T., Okada, N. & Okada, H.: "Expression of HRF20, a regulatory molecule of complement activation, on peripheral blood mononuclear cells" Immunology. 69. 396-401 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Nose, M., Katoh, M., Okada, N., Kyogoku, M. & Okada, H.: "Tissue distribution of HRF20, a novel factor preventing the membrane attack of homologous complement and its predominant expression on endothelial cells in vivo." Immunology. 70. 145-149 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Okada, H., Okada, N. & Hideshima, t.: "HRF20, a membrane inhibitor of complement attack, does not protect cells from the cytotoxic reaction by lymphokine activated killer cells." Biochem. Biophys. Res. Comm.171. 717-721 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Yamashina, M., Ueda, E., Kinoshita, T., Takami, T., Ojima, A., Ono, H., Tanaka, H., Kondo, N., Orii, T., Okada, H., Inoue, K. & Kitan: "Inherited complete deficiency of 20kDa homologous restriction factor (CD59) as a cause of paroxysmal nocturnal hemoglobinuria." N. Eng. J. Med.323. 1184-1189 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Okuda, K., Kanamaru, A., Ueda, E., Kinoshita, T., Okada, N., Okada, H., Kakishta, F. & Nagai, K.: "Expression of decay-accelerating factor on hematopoietic progenitors and their progeny cell growth in cultures withfractionated bone marrow cells from normal individuals and patients with paroxysmal nocturnal hemoglobinuria." Exp. Haematol.18. 1132-1146 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Sayama, K., Shiraishi, S., Shirakata, Y., Kobayashi, Y., Okada, N., Okada, H. & Miki, Y.: "Characterizaion of homologous restriction factor (HRF20) in human skin and white blood cells." Clin. Exp. Immunol.82. 355-358 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Tamai, H., Matsuo, S., Fukatsu, A., Nishikawa, K., Sakamoto, N., Yoshioka, K., Maki, S., Okada, N. & Okada, H.: "Localization of 20kDa homologous restriction factor (HRF20) in deseased human glomeruii--an immunofluorescence study." Clin. Exp. Immunol.84. 256-262 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] McGeer, P. L., Walker, D. G., Akiyama, H., Kawamata, T., Guam, A. L., Perker, C. L., Okada, N. & McGeer, F. G.: "Detection of the membrane inhibitor of reactive lysis (CD59) in deseased neurons of Alzheimer brain." Brain Res.544. 315-319 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Tomita, A., Okada, N. & Okada, H.: "Comparative study of decay-accelerating factor and HLR-DR within the CD8-bright population." Eur. J. Immunol.21. 1843-1848 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Weiss, L., Okada, N., Haeffner-Cavallion, N., Hattori, T., Faucher, C KazatOhkine, M. D. & Okada, H.: "Decreased expression of the membrane inhibitor of complement mediated cytolysis CD59 on T lymphocytes of HIV-infected patients." AIDS.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Holmes, C. H., Simpson, K. I., Okada, H., Okada, N., Wainwright, S. D., Purcell, D. F. I. & Houlihan, J. M.: "Complement regulatory proteins at the feto-maternal interface during human placental development : Distribution of CD59 by comparison with membrane cofactor protein and decay accelerating factor." Eur. J. Jmmunol.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Tomita, A., Okada, N., Watanabe, R., Shinohara, T., Toriya, H., Umeno, T. & Okada, H.: excepta Medica, Amsterdam. Frontiers of Mucosal Immunology(Tsuchiya, M., Magura, H., Hibi, T. & Mori, I., Ed.) Vol. 1. (Altered expression of DAF and HRF20, regulatory molecules of complenetn activation on peripheral blood mononuclear cell in ulcerative colitis.), (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] A.Tomita,N.Okada & H.Okada: "Comparative study of decay accelerating factor and HLA-DR within the CD8-bright population." Eur.J.Immunol. 21. 1843-1848 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] M.Yamashina,E.Ueda,T.Kinoshita,T.Tanami et.al: "Inherited complete deficiency of 20kDa homologous restriction factor(CD59)as a cause of paroxysmal nocturnal hemoglobinuria." New Engl.J.Med.323. 1184-1189 (1990)

    • Related Report
      1991 Annual Research Report
  • [Publications] H.Okada,N.Okada & T.Hideshima: "HRF20,a membrane inhibitor of complement attack,does not protect cells from the cytbtoxic reaction by lymphokine activated killer cells." Biochem.Biophys.Res,Comm.171. 717-721 (1990)

    • Related Report
      1991 Annual Research Report
  • [Publications] R.Harada,N.Okada,T.Fijita & H.Okada: "Purification of 1F5 antigen which prevents complement attack on onhomologosu cell membranes." J.Immunology.144. 1823-1828 (1990)

    • Related Report
      1991 Annual Research Report
  • [Publications] T.Hideshima,N.Okada & H.Okada: "Expression of HRF20,a regulatory molecule of complement activation,on perpheral blood mononuclear cells." Immunology. 69. 396-401 (1990)

    • Related Report
      1991 Annual Research Report
  • [Publications] N.Okada,R.Harada & H.Okada: "Erythrocytes of paroxysmal nocturnal hemoglobinuria patients acqure resistance to complement attack by purified 20kDa homologous restriction factor(HRF20)" Clin.Exp.Immunology.80. 109-113 (1990)

    • Related Report
      1991 Annual Research Report
  • [Publications] A.Tomita,N.Okada,R.Watanabe,T.Shinohara,H.Toriya et.al: "Flontiers of mucosal immundogy Vol 1:Altered expression of DAF & HRF20 regulatory molecules of complement activation on eripheral bood mononuclear cells in Ulcerative colitis." Elsevier Sience Publishers., (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] R.Watanabe,A.Tomita,N.Okada,H.Toriya et.al: "Flontiers of mucosal immundogy Vol 1:Immuno histological study of Ulcerative colitis using monoclonal autibadies against HRF20 and DAF." Elsevier Sience Publishers., (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] 岡田 則子(分担): "Annual Review 免疫 1991:MAC形成阻止糖蛋白" 中外医学社, 370 (1991)

    • Related Report
      1990 Annual Research Report

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Published: 1991-04-01   Modified: 2016-04-21  

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