IMADA Atsushi Sanyo Women's Junior College, Living Department, Assistant Prof., 生活学科, 助教授 (00112201)
TANAKA Junko Hiroshima University, The faculity of Medicine, Assistant, 医学部, 助手 (70155266)
YOSHIZAWA Koji Hiroshima University, The faculity of Medicine, Professor, 医学部, 教授 (30109954)
|Budget Amount *help
¥2,100,000 (Direct Cost: ¥2,100,000)
Fiscal Year 1991: ¥400,000 (Direct Cost: ¥400,000)
Fiscal Year 1990: ¥1,700,000 (Direct Cost: ¥1,700,000)
For increased yield and quality improvement of agricultural products, a wide variety and a large quantity of agricultural chemicals are used in Japan. Safety of such agricultural products when ingested by men is determined by the levels of residual amounts of effective substances of respective agricultural chemicals]However, when such agricultural products are taken by men, m addition to such residual effective substances, products derived from decomposition of organic solvent, emulsifier and stabilizer may become toxic. We conducted following experiment using 3 kinds of organophosphorous insecticide, Fenitrothion, Diazinon and Malathion, and I kind of chlorinated hydrocarbons fungicide (Captan), total 4 kinds of agricultural chemicals.
After diluting those chemicals to respective working concentrations, we exposed them to the sun light for 1, 2, 4 and 8 days for determination of residual amount of effective substances. We, then, tested by Ames test mutagenesis of mixture of residual effective substances and decomposition products.
Fig. 1 shows residual amount of effective substances of 4 kinds of agricultural chemicals on the day 1, 2, 4 and 8.3 or ganophosphorotis chemicals were easily decomposed while chlorinated organic chemical was not.
Table 1 shows the level of mutagenesis of each mixture of residual effective substances and decomposition products.
Chlorinated organic chemical showed high mutagenesis whereas Fenitrothion showed low mutagenesis. Diazation and Malathion, however, showed no mutagenesis. For practical application in our life, further experiments were planned to extract residual substances of agricultural chemicals and their decomposition products absorbed on the fruits in the market. Sudden decease of the leader of this research, Dr. Yukio Hraoka, on October 24, 199 1, however, has made it impossible to continue further qualitative, quantitative and Ames tests of agricultural chemicals.