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Delayed Neurotoxicity of Organophosphorus Compound

Research Project

Project/Area Number 02670226
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field Hygiene
Research InstitutionFukushima Medical College

Principal Investigator

KONNO Nobuhiro  Fukushima Medical College, Dept. of Public Health Assistant Professor, 医学部, 講師 (10045784)

Co-Investigator(Kenkyū-buntansha) KATOH Kiyoshi  Fukushima Medical College, Dept. of Public Health Instructor, 医学部, 助手 (40136974)
YAMAUCHI Toru  Mie University School of Medicine, Dept. of Public Health Professor, 医学部, 教授 (80045674)
Project Period (FY) 1990 – 1991
Project Status Completed (Fiscal Year 1991)
Budget Amount *help
¥2,300,000 (Direct Cost: ¥2,300,000)
Fiscal Year 1991: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 1990: ¥1,800,000 (Direct Cost: ¥1,800,000)
KeywordsOrganophosphate / DFP / Delayed Neurotoxicity / Chicken / OPIDN / Diisopropylfluorophosphate / DFP / ニワトリ
Research Abstract

Binding of [^3H]Diisopropylfluorophosphate(DFP) to membranes from the chicken spinal cord has been studied. [^3H]DFP binding was specific, saturable and reversible. [^3H]DFP binding sites behaved like a receptor site(s). Scatchard plots revealed two classes of binding sites. The competition curves showed that [^3H]DFP binding was inhibited by the delayed neurotoxic compound, such as DFP, mipafox and leptophos. It was also inhibited by paraoxon. Paraoxon might act as an antagonist to the binding sites. Autoradiography of [^3H]DFP binding in spinal cord slices showed higher levels of binding sites in gray matter compared with white matter. A single iv dosing of 40 mg/kg of tri-o-cresyl phosphate (TOCP) to chickens resulted in more than 70% decrease of [^3H]DFP binding sites in spinal cord membranes after 6 to 24 hr. This suggests that [^3H]DFP binding sites might be involved in the initial target of organophosphate induced delayed neurotoxicity (OPIDN).

Report

(3 results)
  • 1991 Annual Research Report   Final Research Report Summary
  • 1990 Annual Research Report
  • Research Products

    (14 results)

All Other

All Publications (14 results)

  • [Publications] Konno,N.,Katoh,K.,Yamauchi,T.,and Fukushima,M.: "Delayed neurotoxicity of triphenyl phosphite in hens:pharmacokinetic and biochemical studies." Toxicol.Appl.Pharmacol.100. 440-450 (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Katoh,k.,Konno,N.,Yamauchi,T.,and Fukushima,M.: "Effects of age on susceptibility of chickens to delayed neurotoxicity due to triphenyl phosphite." Pharmacology and Toxicology. 66. 387-392 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] 白坂 正良,紺野 信弘: "生体試料中の8種有機リン殺虫剤の簡易定量法ー中毒実験利用を目的としてー." 衛生化学. 36. 338-343 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Katoh,K.: "Age-related differences in the inhibition of neuropathy target esterase and susceptibility to triphenyl phosphite-induced delayed neurotoxicity in chickens." Jpn.J.Hyg.47. 861-870 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Konno,N.,Katoh,K.,Yamauchi,T.,and Fukushima,M.: "Distribution and Specific binding of[^3H]diisopropylfluorophosphate(DFP)in chicken nervous tissues." Environmental Sciences. 1. 155-168 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] 紺野 信弘: "有機リン殺虫剤の遅発性神経毒性" 宮城県獣医師会会報. 45. 108-116 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Yamauchi,T., Katoh,K., Konno,N. and Fukushima,M.: "Delayed neurotoxicity and toxicokinetics of leptophos in hens given repeatedly by low-dose intravenous injections." J. Toxicol. Sci.14. 11-21 (1989)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Konno,N., Katoh,K., Yamauchi,T. and Fukushima,M.: "Delayed neurotoxicity of triphenyl phosphite in hens : pharmacokinetic and biochemical studies." Toxicol. Appl. Pharmacol.100. 440-450 (1989)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Katoh,K., Konno,N., Yamauchi,T. and Fukushima,M.: "Effects of age on susceptibility of chickens to delayed neurotoxicity due to triphenyl phosphite." Pharmacology and Toxicology. 66. 387-392 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Katoh,K.: "Age-related differences in the inhibition of neuropathy target esterase and susceptibility to triphenyl phosphite-induced delayed neurotoxicity in chickens." Jpn. J. Hyg.47. 861-870 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Konno,N., Katoh,K., Yamauchi,T. and Fukushima,M.: "Distribution and specific binding of [^3H]diisopropylfluorophosphate (DFP) in chicken nervous tissues." Environmental Sciences. 1. 155-168 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Nobuhiro Konno et al: "Distribution and specific binding of [ ^3H]diisopropylfluorophosphate(DFP)to Chicken nervous tissues" Environmental Sciences.

    • Related Report
      1991 Annual Research Report
  • [Publications] Kiyoshi Katoh: "Ageーrelated differences in the inhibition of neuropathy target esterase and susceptibility to triphenyl phosphiteーinduced delayed neurotoxicity in chickens" 日本衛生学雑誌(英文).

    • Related Report
      1991 Annual Research Report
  • [Publications] Nobuhiro Konno: "Covalent and specific binding of [^3H]diisopropylfluorophosphate(DFP)to nervous tissues of chickens" Toxicology and Applied Pharmacology.

    • Related Report
      1990 Annual Research Report

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Published: 1990-04-01   Modified: 2016-04-21  

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