Immunological studies on stinging insect hypersensitivity
| Project/Area Number |
02670261
|
|---|
| Research Category |
Grant-in-Aid for General Scientific Research (C)
|
| Allocation Type | Single-year Grants |
|---|
| Research Field |
Legal medicine
|
|---|
| Research Institution | Kagawa Medical School |
|---|
Principal Investigator |
AMENO Setsuko Kagawa Medical School, Forensic Medicine, Research Associate, 医学部, 助手 (00032904)
|
|---|
| Project Period (FY) |
1990 – 1991
|
| Project Status |
Completed (Fiscal Year 1991)
|
| Budget Amount *help |
¥2,200,000 (Direct Cost: ¥2,200,000)
Fiscal Year 1991: ¥400,000 (Direct Cost: ¥400,000)
Fiscal Year 1990: ¥1,800,000 (Direct Cost: ¥1,800,000)
|
|---|
| Keywords | Bee sting allergy / Shock / Bee venom specific IgE / Bee venom specific IgG4 / ELISA / アナフィラキシ-ショック / venom IgG_4 / venom IgE / ELISA |
|---|
| Research Abstract |
In Japan, an average of 37 fatalities per year due to bee or wasp stings were reported during the years 1979-1988. In order to confirm fatal anaphylaxis serologically, we used ELISA(Enzyme-Linked Immunosorvent Assay) to measure bee venom specific IgE(sIgE) and IgG4(sIgG4) antibodies in the sera of 22 patients who visited hospitals with allergy reactions or anaphylactic reactions after bee or wasp stings. Specific IgE was detected in 12 patients(52%) and sIgG4 was detected in 8 patients(35%). Both anti-venom antibodies were detected at significant level but having no relation to the degree of allergic reactions. However, especially high levels of sIgE and sIgG4 were detected in patients experiencing the most severe clinical reactions. Venom specific IgE and IgG4 appear to be useful when determining bee or wasp stings as the cause of fatalities
The venoms of (1) Apis mellifera (Dried whole venom ; HB), (2) Apis mellifera (Phospholipase A2 ; PLA2), (3) Dolicovespula arenaria (Yellow hornet ; YH), (4) Polisters apachus (Paper wasp ; PW), (5) A mixture of Polistes annularis, P. exelamans, P. fuscatus and P. metricus, and (6) A mixture of Vespula germanica and Vespula vulgaris were used as antigens. The venoms of HB, PLA2, YH, and PW were compared by isoelectric focusing (pH 3.5-9.5). Although a few common bands were detected, these venoms silver-stained banding patterns were different.
|
Report
(3 results)
Research Products
(4 results)
