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Biochemical Studies on Liver Functions in Primary Cultured Hepatocytes of Old Rats

Research Project

Project/Area Number 02670305
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field Gastroenterology
Research InstitutionTottori University

Principal Investigator

MURA Tetsuo  Division of Chemistry, Steroid Research, Tottori University School of Medicine, Lecture, 医学部, 講師 (80093631)

Project Period (FY) 1990 – 1992
Project Status Completed (Fiscal Year 1992)
Budget Amount *help
¥600,000 (Direct Cost: ¥600,000)
Fiscal Year 1992: ¥300,000 (Direct Cost: ¥300,000)
Fiscal Year 1991: ¥300,000 (Direct Cost: ¥300,000)
KeywordsPrimary cultured of old rat hepatocytes / Low cell density culture / Fatty acid synthesis by glucagon / Bile acid conjugation / Peroxisome and bile acid synthesis / Desmosterol conversion / Hydroxylation of bile acid by dexamethasone / EGF receptor assay / グルカゴンの脂肪酸合成誘導能 / デスモステロール / グリシン / タウリン抱合胆汁酸比 / デキサメタゾンによる胆汁酸合成 / 老齢ラット肝機能 / 初代培養肝細胞 / 細胞密度依存性 / 細胞表層膜蛋白質 / 肝細胞増殖能 / 多層集合体形成能 / タンパク質リン酸化 / 5^1ーヌクレオチダ-ゼ / 老齢ラット初代培養肝細胞 / 細胞密度依存性肝機能 / 肝細胞膜蛋白質 / 胆汁酸抱合能 / コレステロ-ル代謝 / コレステロ-ル胆石症
Research Abstract

Many metabolic functions besides proliferation are regulated by cell density and that there is a reciprocal relation between cell growth and expression of hepatocyte-specific functions. On the other hand, old rat hepatocytes in primary culture are well known not to respond normally to EGF. Therefore, we examined whether any reciprocal related between cell growth and expression of hepatocyte-specific functions could be demonstrated in old rat hepatocytes by change in cell density or addition of plasma membranes protein from adult and old rat liver.
The following results were obtained : (1)The effect of glucagon on fatty acid synthesis at old rat hepatocytes was lower than that of adult rats. (2)Norepinephrine stimulation of DNA synthesis was lower at the presence of insulin and EGF (3)When the old rat hepatocytes were cultured at low cell density, the dexamethasone increased taurin conjugation of the dihydroxylated bile acids, and reversibly the hydroxylation of deoxycholate in the 6 beta and 7alpha position was decreased.
These results are interesting to speculate on how old hepatocytes lose the ability to respond to the cell-surface mulator during aging. Cell-cell contact could be considered as being analogues to the interaction of hormones with surface receptors.
The interaction between cells could produce an intracellular signal that is responsible for suppression of cell growth and expression of tissue-specific functions.

Report

(4 results)
  • 1992 Annual Research Report   Final Research Report Summary
  • 1991 Annual Research Report
  • 1990 Annual Research Report
  • Research Products

    (12 results)

All Other

All Publications (12 results)

  • [Publications] Y.KOHNO: "The Effect of Human Milk on DNA Synthesis of Neonatal Rat Hepatocyte in Primary Culture" Pediatric Research. 29. 251-255 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Y.KOHNO: "Iron-Saturated Lactoferrin as a Co-Mitogenic Substance for Neonatal Rat Hepatocytes in Primary Culture" Acta Pediatrica.

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Y.Kohno: "The Effect of Human Milk on DNA synthesis of Neonatal Rat Hepatocytes in Primary Culture" Pediatric Research. 29. 251-255 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Y.Kohno: "Iron-Saturated Lactoferrin as a Co-Mitogenic Substance for Neonatal Rat Hepatocytes in Primary Culture" Acta Pediatrica.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Y Kohno: "The Effect of Human Milk on DNA Synthesis of Neonatal Rat Hepatocytes in Primary Culture" Pediatric Research. 29. 251-255 (1991)

    • Related Report
      1992 Annual Research Report
  • [Publications] Y Kohno: "Iron-Saturated Lactoferrin as a Co-Mitogenic Substance for Neonatal Rat Hepatocytes in Primary Culture" Acta Pediatrica.

    • Related Report
      1992 Annual Research Report
  • [Publications] 武良 哲雄: "Reciprocal modulation of liver functions of primary cultured old rat hepatocytes by contact with cells or cell membranes"

    • Related Report
      1991 Annual Research Report
  • [Publications] 武良 哲雄: "DensityーDependent Hepatocyte Functions of Old Rat in Primary Culture"

    • Related Report
      1991 Annual Research Report
  • [Publications] 武良 哲雄: "Use of hepatocytes in spheroid formation for biochemical studies on old rat liver functions"

    • Related Report
      1991 Annual Research Report
  • [Publications] 武良 哲雄: "Modulation of bile acid conjugation of old rat hepatocytes in primary culture by cellーcell contact and cell membranes"

    • Related Report
      1990 Annual Research Report
  • [Publications] 武良 哲雄: "Membrane characteristic of old rat liver parenchymal in primary monolayer culture"

    • Related Report
      1990 Annual Research Report
  • [Publications] 武良 哲雄: "老齢および成熟ラットの初代培養肝細胞による肝機能の生化学的研究"

    • Related Report
      1990 Annual Research Report

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Published: 1991-04-01   Modified: 2016-04-21  

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