| Project/Area Number |
02670368
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
Neurology
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| Research Institution | AICHI MEDICAL UNIVERSITY |
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Principal Investigator |
MITSUMA Terunori DEPARTMENT OF INTERNAL MEDICINE PROFESSOR, 医学部, 教授 (00111857)
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| Co-Investigator(Kenkyū-buntansha) |
KAYAMA Masato DEPARTMENT OF INTERNAL MEDICINE ASSISTANT, 医学部, 助手 (60177615)
NAKAO Naoki DEPARTMENT OF INTERNAL MEDICINE ASSISTANT PROFESSOR, 医学部, 講師 (00140038)
HIROOKA Yoshifumi DEPARTMENT OF INTERNAL MEDICINE ASSOCIATE PROFESSOR, 医学部, 助教授 (90126821)
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| Project Period (FY) |
1990 – 1992
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| Project Status |
Completed (Fiscal Year 1992)
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| Budget Amount *help |
¥2,400,000 (Direct Cost: ¥2,400,000)
Fiscal Year 1992: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 1991: ¥900,000 (Direct Cost: ¥900,000)
Fiscal Year 1990: ¥800,000 (Direct Cost: ¥800,000)
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| Keywords | neuropeptide / beta-endorphin / cerebrovascular disorder / TRH / substance P / somatostatin / gracil axonal dystrophy mouse / エンドセリン111 / エンドセリンIII / βーエンドルフィン / 薄束ジストロフィ-マウス / TRH関連関質 |
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| Research Abstract |
In order to clarify the pathophysiological significance of neuropeptide on the central nervous system disease, pathophysiological, immunohistochemical and clinical studies were made. We newly developed radioimmunoassay for endothelin I, pre-pro-TRH (178-199) and TRH-glycine, and these assay systems are very specific and reliable. Using these assay systems, endothelin I, pre-pro-TRH (178-199) and TRH-glycine were found to be widely distributed in the rat body. By an immunohistochemical analysis, endothelin III was widely distributed in the rat body. Meclofenoxate and bifemelane act on the anterior pituitary and reduce plasma beta-endorphin levels, while clothiapine acts on the anterior pituitary to increase plasma beta-endorphin levels in rats. Plasma beta-endorphin levels increased significantly after cerebrovasucular attack and then return to pre-attack levels. In gracil axonal dystrophy mouse, TRH, somatostain and substance P concentrations in the brain changed and the regions of changes in the peptides are nearly correlated with the pathological changes of this mice. From these findings it is suggest that neuropeptide may play important role in central nervous system disease.
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