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In vitro detection of antigen sensitization by bioluminiscence and its clinical application.

Research Project

Project/Area Number 02670428
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field Pediatrics
Research InstitutionUniversity of Tokyo

Principal Investigator

HAYAKAWA Hiroshi  University of Tokyo, Faculty of Medicine, Associate Professor, 医学部(方), 助教授 (80107507)

Co-Investigator(Kenkyū-buntansha) TAKAMIZAWA Masaru  University of Tokyo, Faculty of Medicine, Fellow, 医学部(方), 助手 (40216784)
KOMODA Fusako  University of Tokyo, Faculty of Medicine, Fellow, 医学部(方), 助手 (50107603)
IWATA Tsutomu  University of Tokyo, Faculty of Medicine, Assistant Professor, 医学部(方), 講師 (00134578)
Project Period (FY) 1990 – 1991
Project Status Completed (Fiscal Year 1991)
Budget Amount *help
¥2,300,000 (Direct Cost: ¥2,300,000)
Fiscal Year 1991: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 1990: ¥1,300,000 (Direct Cost: ¥1,300,000)
KeywordsChemiluminiscence / Phytohaemagglutinin / Ovoalubumin / Lactoalbumin / Food allergy / Atopic disease / リンパ球 / 好中球 / 食物アレルギ- / FMLP / アレルギ-疾患
Research Abstract

Chemiluminiscence response of peripheral white blood cells under stimulation by phytoheamagglutinin and FMLP was investigated in the infants with atopic diseases related to food allergy, controled with healty adults and immunodeficient children. Similar to FMLP,phytohaemaggulutinin stimulated significant chemiluminiscence in the healthy adults and atopic infants, however no response was observed in the patients with cellular immunodeficiency. This results suggested that this method might be applicable as a simple clinical examination to detect cellular immunity of the donors.
Chomiluminiscence response of peripheral white blood cells under stimulation by the antigens which sensitize the donors previously was also investigated in the infants with atopic diseases. The subjects were selected from the paticipants of the official project "Health consultation and follow-up survey of atopic infants", projected and supported by Department of Health and Welfare, Bunkyo-ku. The antigens, ovoalbumin for egg-allergy and lactoalbumin for cow-milk allergy, stimulated significant chemiluminiscence response in some of the sensited infants, however the response was not so remarkable as phytohaemagglutinin and FMLP.
Althogh the results obtained seemed to be not enough this preliminary study suggested the possibility of application of chemiluminiscence to diadnosis food allergy and other complicated allergy, and the further investigation is promising.

Report

(3 results)
  • 1991 Annual Research Report   Final Research Report Summary
  • 1990 Annual Research Report
  • Research Products

    (3 results)

All Other

All Publications (3 results)

  • [Publications] 岩田力: "東京都文京区におけるアレルギー健康調査について(第2報)" 小児科臨床. 44. 2645-2650 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Tsutomu Iwata et al.: "Health consultation and follow-up survey of stopic infants in Bunkyoku, Tokyo (Second report). (in Japanese)" Shonika-Rinsho. 44. 2645-2650 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] 岩田力: "東京都文京区におけるアレルギ-健康診査について" 小児科臨床. 43. 2645-2650 (1991)

    • Related Report
      1991 Annual Research Report

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Published: 1990-04-01   Modified: 2016-04-21  

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