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Differentiation and maturation of human neuroblastoma into smooth muscle cells

Research Project

Project/Area Number 02670456
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field Pediatrics
Research InstitutionKyoto Prefectural Univ. of Medicine

Principal Investigator

SUGIMOTO Tohru  Kyoto Prefectural Univ. of Medicine Assistant Prof., 医学部, 講師 (90117888)

Project Period (FY) 1990 – 1991
Project Status Completed (Fiscal Year 1991)
Budget Amount *help
¥2,300,000 (Direct Cost: ¥2,300,000)
Fiscal Year 1991: ¥900,000 (Direct Cost: ¥900,000)
Fiscal Year 1990: ¥1,400,000 (Direct Cost: ¥1,400,000)
Keywordsnuroblastoma / Smooth muscle cell / Differentiation
Research Abstract

The neural crest gives rise to a variety of tissues, including peripheral neurons, Schwann cells, melanocyles and ectomesencymal cells, which include the smooth muscle cells of large arteries. Cell lines derived from neuroblastoma (a neural crest tumor) have at least two distinct morphological cell types, a neuroblastic phenotype (N-type) and an epithelial-like phenotype (S-type) with characteristics of substrate-adhesiveness. We have analyzed 17 human neuroblastoma cell lines using a panel of monoclonal antibodies against cytoskeletal proteins. Three neuroblastoma cell lines (KP-N-SI, KP-N-YN and SMS-KCN) bound an alpha-smooth muscle acting antibody. In addition, one of these cell lines( KP-N-SI) bound anti-desmin monoclonal antibodies as determined by indirect immunoflourescence. A total of eight cloned cell lines were obtained from the above parent cell lines. These were composed of either N- or S-type cells and were confirmed to be the common neuroblastoma origin from each parent cell line by chromosomal analysis. Alpha-smooth muscle actin and desmin were demonstrated in the S-type cloned cells by indirect immunofluorescence, as well as by two-dimensional Western blot analysis. These results were confirmed by Northern blot analysis using a specific probe (pSHalphaSMA-3'UT) to human alpha-smooth muscle actin mRNA. This is the first report of the presence of alpha-smooth muscle actin and desmin in neuroblastoma cell lines. These data show that in addition to giving rise to cells with neural, Schwann cell and melanocyte markers, neuroblastoma can also give rise to the cells expressing smooth muscle cell marke.

Report

(3 results)
  • 1991 Annual Research Report   Final Research Report Summary
  • 1990 Annual Research Report
  • Research Products

    (19 results)

All Other

All Publications (19 results)

  • [Publications] T.Sugimoto: "Alpha-smooth-muscle actin and desmin expressions in human neuroblastoma cell lines" Int.J.Cancer. 48(1). 277-283 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] H.Kuroda: "Different drug sensitivity in two neuroblastoma cell lines established from the same patient before and after chemotherapy" Int.J.Cancer. 47(2). 732-737 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] T.Sugimoto: "Neuronal differentiation of human neuroblastoma cells by retinoid" J.Nutritional Science Vitaminology. (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] 杉本 徹ら: "細胞表面膜抗原,細胞骨格蛋白とNーmrc癌遺伝子による小児固形悪性腫瘍の診断と問題点" Human Cell. 5(1). 1 1-11 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] 杉本 徹ら: "平滑筋細胞マ-カ-を持つヒト神経芽腫細胞株" Human Cell. 4(3). 248-256 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] T. Sugimoto et al.: "Alpha-smooth-muscle actin and desmin expressions in human neuroblastoma cell lines." Int. J. Cancer. 48(1). 277-283 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] H. Kuroda, T. Sugimoto et al.: "Different drug sensitivity in two neuroblastoma cell lines established from the same patient before and after chemotherapy." Int. J. Cancer. 47(2). 732-737 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] T. Sugimoto et al.: "Neuronal differentiation of human neuroblastoma cells by retinoid." J. Nutritional Science Vitaminology. (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] T.Suhimoto: "Alphaーsmoothーmuscle actin and desmin expressions in human neuroblastoma cell lines" Int.J.Cancer. 48(1). 277-283 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] H.Kuroda: "Different drug sensitivity in two neuroblastoma cell lines established from the same patient before and after chemotherapy" Int.J.Cancer. 47(2). 732-737 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] T.Sugimoto: "Neuronal differentiation of human neuroblastoma cells by retinoid" J.Nutritional Science Vitaminology. (1992)

    • Related Report
      1991 Annual Research Report
  • [Publications] 杉本 徹ら: "細胞表面膜抗原,細胞骨格蛋白とNーmyc癌遺伝子による小児固形悪性腫瘍の診断と間題点" Human Cell. 5(1). 11-11 (1992)

    • Related Report
      1991 Annual Research Report
  • [Publications] 杉本 徹ら: "平滑筋細胞マ-カ-を持つヒト神経芽腫細胞株" Human Cell. 4(3). 248-256 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] T.Sugimoto: "Alphaーsmooth muscle actin and desmin expressions in human neuroblastoma cell lines" Int.J.Cancer. (1991)

    • Related Report
      1990 Annual Research Report
  • [Publications] H.Kuroda: "Different drug sensitivity in two neuroblastoma cell lines established from the same patient before and after chemotherapy" Int.J.Cancer. (1991)

    • Related Report
      1990 Annual Research Report
  • [Publications] Y.Hayashi: "characterization of an embryonal rhabdomyosarcoma cell line showing amplification and overーexpression of the Nーmyc oncogene" Int,J.Cancer. 45. 705-711 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] 杉本 徹: "神経芽腫の分化誘導:腫瘍生物学的特性と臨床応用の可能性" Biotherapy. 4. 1007-1018 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] 杉本 徹: "α平滑筋アクチンとデスミン陽性ヒト神経芽腫細胞株" 医学のあゆみ. 153. 273-274 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] 杉本 徹: "Granulocyte colonyーstimulating factorがヒト神経芽腫細胞コロニ-形成に及ぼす影響" 医学のあゆみ. 153. 317-318 (1990)

    • Related Report
      1990 Annual Research Report

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Published: 1990-04-01   Modified: 2016-04-21  

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