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RESEARCH FOR TRANSMEMBRANE SIGNALING SYSTEM OF EPIDERMAL KERATINOCYTES; INTRACELLULAR FREE CALCIUM AND CELLULAR RESPONSES.

Research Project

Project/Area Number 02670469
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field Dermatology
Research InstitutionHOKKAIDO UNIVERSITY

Principal Investigator

KOIZUMI Hiroko  HOKKAIDO UNIVERSITY MEDICAL HOSPITAL, DERMATOLOGY, LECTURER, 医学部附属病院, 講師 (60113552)

Co-Investigator(Kenkyū-buntansha) YASUI Chikako  HOKKAIDO UNIVERSITY SCHOOL OF MEDICINE, DERMATOLOGY, ASSISTANT, 医学部, 助手 (50210240)
FUKAYA Toru  HOKKAIDO UNIVERSITY SCHOOL OF MEDICINE, DERMATOLOGY, ASSISTANT, 医学部, 助手 (90133727)
Project Period (FY) 1990 – 1992
Project Status Completed (Fiscal Year 1992)
Budget Amount *help
¥2,600,000 (Direct Cost: ¥2,600,000)
Fiscal Year 1992: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 1991: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 1990: ¥1,100,000 (Direct Cost: ¥1,100,000)
KeywordsKERATINOCYTE / CALCIUM / PEROXIDE / SUBSTANCE P / PHOSPHOLIPASE C / PROTEIN KINASE C / ADENYLATE CYCLASE / PSORIASIS / Protein kinase C / phospholipase C / protein kinase C / adenylate cyclase / 細胞内遊離カルシウム / リポコルチン / フォドリン / エピネフリン
Research Abstract

Epidermal keratinocytes proliferate and differentiate to make stratum corneum. Many chemical mediators, hormones and peptides stimulate keratinocytes to induce phosphophatidylinositol metabolism, alteration of intracellular free calcium, adenylate cyclase response activation. We elucidated that bradykinin, thrombin, substance P, beta-adrenarin, adenosine, and histamine induce increase of intracellular free calcium. These effects are mediated with synthesis of inositol 1,4,5-trisphosphate or adenylate cyclase activation. The importance of calcium as a second messenger is shown. Presence of membrane skeleton proteins such as spectrin, b-fodrin, 4.1 protein like proteins were detected immunologically and the alteration of their distribution in cultured keratinocytes were observed with the change of calcium concentration of the medium. A new technique was reported to detect peroxide produced from keratinocytes using dihydrorhodamine 123.
Cross-talk among transmembrane signaling systems in keratinocytes, and drugs to influence these systems should be investigated. Abnormal keratinization and acceleration of proliferation are considered to closely realt to pathogenesis of psoriasis. The alteration of membrane signaling systems in psoriasis and the action mechanisms of the drugs used in therapy of skin diseases are under investigation.

Report

(4 results)
  • 1992 Annual Research Report   Final Research Report Summary
  • 1991 Annual Research Report
  • 1990 Annual Research Report
  • Research Products

    (77 results)

All Other

All Publications (77 results)

  • [Publications] KOIZUMI H.: "Substance P induces intracellular Ca^<++>increase and protein kinase C avtivation in the epidermis." British Journal of Dermatology. 127. 595-599 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] KOIZUMI H.: "Effect of novel synthesized retino-benzoic acid(Am-80)on adenylate cyclase and arachidonic release of pig epidermis." Journal of Dermatological Science. 3. 97-102 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] YASUI C: "Adenylate cyclase induces intracellular calcium increase measured by imaging fluorography using Fura2-AM." British Journal of Dermatology. 127. 589-594 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] SAKURADA H.: "Use of dihydrorhodamine123for detecting intracellular generation of peroxide upon UV irradiation in epidermal keratinocytes." Archives of Dermatological Research. 284. 114-116 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] SHIMIZU T.: "Localization of proteins immunologically related to erythrocyte protein4.1,spectrin and ankyrin thyroid gland." Acta Histochemica. 93. 441-445 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] KOIZUMI H.: "Adenosine deaminase activity in serum of patients with psoriasis,mycosis fungoides,and adult T ccll leukemia." Acta Dermatovenereologica(Stockh). 72. 410-412 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] 小泉 洋子: "表皮細胞情報伝達系とカルシウム." 皮膚診療. 14. 402-405 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] 小泉 洋子: "角化異常とレチノイド." FRAGRANCE JOURNAL.20. 26-31 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] 飯塚 一: "表皮細胞における核酸の生合成と代謝." FRAGRANCE JOURNAL.20. 41-47 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] 小泉 洋子: "neuropeptide." 皮膚科の臨床.

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] 小泉 洋子: "Protein kinase C." 皮膚科の臨床.

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] 小泉 洋子: "情報伝達系" 日本皮膚科学会雑誌.

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] KOIZUMI H.: "clinical significance of serum adenosine deaminase activity in patients with mycosis fungoides." Journal of Dermatology.

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] SHIMIZU T.: "Calcium dependent formation of 4.1-like protein in cultured human keratinocytes." Histochemistry.

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] SHIMIZU T.: "Immunoreactive analogues of erythrocyte ankyrin in human epidermal keratinocyte." Arch Derm Res.

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] OSADA S.: "predominant expression of nPKCη,aCa^<2+>-independent isoform of protein kinase C in epithelial tissues,in association with epithelial differentiation." Cell Growth and Differentiation.

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] 小泉 洋子: "乾癬.Today's therapy1993今日の治療指針" 医学書院, (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Koizumi H, Fukaya T, Tanaka H, Ohkawara A,Ueda T:"Substance Pinduces intracellular calicium increase and protein kinase C activation in the epiudermis." British Journal of Dermatology. 127. 595-599 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Koizumi H, Sato KC, Ohkawara A: "Effect of novel synthesized retinobenzoic acid (Am-80) on adenylate cyclase and arachidonic acid release of pig epiudermis." Journal of Dermatological Research. 3. 97-102 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Yasui C, Koizumi H, Fukaya T, Ohkawara A, Ueda T:"Adenylate cyclase induces intracellular calcium increase measured by imaging fluorography using Fura 2-AM." British Journal of Dermatology. 127. 589-594 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Sakurada H, Koizumi H, Ohkawara A, Ueda T, Kamo N: "Use of dihydrorhodamin 123 for detecting intracellula generation of peroxide upon UV irradiation in epidermal keratinocytes." Archives of Dermatological Research. 284. 114-116 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Shimizu T, Takakuwa Y, Koizumi H, Ishibashi T, Ohkawara A: "Localization of proteins immunologically related to erythrocyte protein 4.1, spectrin and ankyrin n thyroid gland." Acta Histochemica. 93. 441-445 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Koizumi H, Ohkawara A: "Adenosine deaminase activity in serum of patients with psoriasis, mycosis fungoides, and adult T cell leukemia." Acta Dermatovenereologica(Stockh). 72. 410-412 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Koizumi H: "Signal transduction and calcium in epidermal keratinocytes." Hihu Shinryo. 14. 402-405 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Koizumi Hiroko: "Disorder of keratinization and retinoid" Fragrance Journal. 20. 26-31 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Iizuka H:"Nucleic acid metabolism in epidermal keratinocytes" Fragrance Journal. 20. 41-47 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Koizumi H: "Neuropeptide" Hihu Rinshou.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Koizumi H: "Protein kinase C" Hihu Rinshou.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Koizumi H: "signal transduction in epidermal keratinocytes." Japanese Journal of Dermatology.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Koizumi H, Tomizawa K, Tanaka H, Kumakiri M, Ohkawara A: "Clinical significance of serum adenosine deaminase activiy in patients with mycosis fungoides." Journal of Dermatology.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Shimizu T, Takakuwa Y, Koizumi H, Ishibashi T, Ohkawara A: "Calcium dependent formation of 4.1-like protein in cultured human keratinocytes." Histochemistry.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Shimizu T, Takakuwa Y, Koizumi H, Ishibashi T, Ohkawara A: "Immunoreactive analogues of erythrocyte ankyrin in human epidermal keratinocyte." Archives of Dermmatological Research.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] OsadaS,Hashimoto Y, Nomura S, Kohno Y, Chida K, Tajima O, Kubo K, Akimoto K, Koizumi H, Kitamura S, Suzuki K, Ohno S, Kuroki T: "Predominant expression of nPKCeta, a Ca^<2+>independent isoform of protein kinase C in epithelial tissues, in association with epithelial differentiation." Cell Growth and Differentiation.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Koizumi H: "psoriasis" Today's therapy 1993.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Koizumi H.: "Substance P induces intracellular Ca^<++> increase and protein kinase C avtivation in the epidermis." British Journal of Dermatology. 127. 595-599 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] Koizumi H.: "Effect of novel synthesized retino-benzoic acid(Am-80) on adenylate cyclase and arachidonic release of pig epidermis." Journal of Dermatological Science. 3. 97-102 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] Yasui C.: "Adenylate cyclase induces intracellular calcium increase measured by imaging fluorography using Fura 2-AM." British Journal of Dermatology. 127. 589-594 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] Sakuraba H.: "Use of dehydrorhodamine 123 for detecting intracellular generation of peroxide upon UV irradiation in epidermal keratinocytes." Archives of Dermatological Research. 284. 114-116 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] Shimizu T.: "Localization of proteins immunologically related to erythrocyte protein 4.1,spectrin and ankyrin in thyroid gland." Acta Histochemica. 93. 441-445 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] Koizumi H.: "Adenosine deaminase activity in serum of patients with psoriasis,mycosis fungoides,and adult T cell leukemia." Acta Dermatovenereologica. 72. 410-412 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] 小泉 洋子: "表皮細胞情報伝達系とカルシウム." 皮膚診療. 14. 402-405 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] 小泉 洋子: "角化異常とレチノイド." FRANGANCE JOURNAL. 20. 26-31 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] 飯塚 一: "表皮細胞における核酸の生合成と代謝" FRAGANCE JOURNAL. 20. 41-47 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] 小泉 洋子: "neuropeptide." 皮膚科の臨床.

    • Related Report
      1992 Annual Research Report
  • [Publications] 小泉 洋子: "Protein kinase C." 皮膚科の臨床.

    • Related Report
      1992 Annual Research Report
  • [Publications] 小泉 洋子: "情報伝達系." 日本皮膚科学会雑誌.

    • Related Report
      1992 Annual Research Report
  • [Publications] Koizumi H.: "clinical significance of serum adenosine deaminase activity in patients with mycosis fungoides." Journal of Dermatology.

    • Related Report
      1992 Annual Research Report
  • [Publications] Shimizu T.: "Calcium dependent formation of 4.1-like protein in cultured human keratinocytes." Histochemistry.

    • Related Report
      1992 Annual Research Report
  • [Publications] Shimizu T.: "Immunoreactive analogues of erythrocyte ankyrin in human epidermal keratinocyte." Arch Derm Res.

    • Related Report
      1992 Annual Research Report
  • [Publications] Osada S.: "predominant expression of nPKCη,a Ca^<2+>-independent isoform of protein kinase C in epithelial tissues,in association with epithelial differentiation." Cell Growth and Differentiation.

    • Related Report
      1992 Annual Research Report
  • [Publications] 小泉 洋子: "乾癬(Today's therapy 1993 今日の治療指針)" 医学書院,

    • Related Report
      1992 Annual Research Report
  • [Publications] 小泉 洋子: "乾癬(Today's therapy 1993 今日の治療指針)" 医学書院, (1993)

    • Related Report
      1992 Annual Research Report
  • [Publications] Yoshifumi Osawa: "Adenylate cyclase induces intracellular Ca^<2+> increase in single human epidermal keratinocytes of the epidermal sheet as measured by digital imaging microscopy using Fura 2ーAM" Archives of Dermatological Research. 283. 91-95 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] Tadamichi Shimizu: "Presence and localization of proteins immunologically related to erythrocyte protein 4.1 in skin" Histochemistry. 95. 549-554 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] 小泉 洋子: "乾癬に対するエイコサペンタエン酸内服・外用治療効果" 日本皮膚科学会雑誌. 101. 1723-1726 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] 小泉 洋子: "角化異常とレテノイド" FREGRANCE JOURNAL. 20. 26-31 (1992)

    • Related Report
      1991 Annual Research Report
  • [Publications] Hiroko Koizumi: "Substance P induces intracellular calcium increase and translocation of protein kinase C in the epidermis" Journal of Investigative Dermatology. 98. (1992)

    • Related Report
      1991 Annual Research Report
  • [Publications] Hitoshi Sakurada: "Use of dihydrorhodamine 123 for detecting intracellular generation of peroxides upon UV irradiation in epidermal keratinocytes" Archives of Dermatological Research. 284. (1992)

    • Related Report
      1991 Annual Research Report
  • [Publications] 飯塚 一: "表皮細胞における核酸の生合成と代謝" FRAGRANCE JOURNAL. 20. 41-47 (1992)

    • Related Report
      1991 Annual Research Report
  • [Publications] Hiroko Koizumi: "Effects of novel synthesized retinobenzoic acid(Amー80)on adenylate cyclase and arachidonic acid release in pigepidermis" Journal of Dermatological Science. 3. (1992)

    • Related Report
      1991 Annual Research Report
  • [Publications] 小泉 洋子: "表皮細胞情報伝達系とカルシウム" 皮膚病診療. 14. (1992)

    • Related Report
      1991 Annual Research Report
  • [Publications] Yoshifumi Osawa: "Adenylatecyclase induces intracellular Ca^<2+> increase in single human epidermal keratinocytes of the epidermal sheet as measured by digital imaging microscopy using fura 2ーAM" Dermatology Digest. 2. 14 (1992)

    • Related Report
      1991 Annual Research Report
  • [Publications] Tadamichi Shimizu: "Calcium dependent formation of 4.1ーlike proteins in cultured human keratinocytes" Archives of Dermatological Research.

    • Related Report
      1991 Annual Research Report
  • [Publications] Hiroko Koizumi: "Adenosine deaminase activity in sera of patients with psoriasis,mycosis fungoides,and adult T cell leukemia"

    • Related Report
      1991 Annual Research Report
  • [Publications] Hiroko Koizumi: "Substance P induces intracellular calcium increase and translocation of protein kinase C in epidermis" submitted.

    • Related Report
      1991 Annual Research Report
  • [Publications] Junーichiro Takano: "Ultraviolet action spectra for peroxide generation in human and pig epidermal keratinocytes loaded with dihydrorhodamine 123" submitted.

    • Related Report
      1991 Annual Research Report
  • [Publications] Chikako Yasui: "Adenylate cyclase induces intracellular Ca^<2+> increase in single human epidermal keratinocytes measured by the fluorescence microscopy using Fura 2ーAM" submitted.

    • Related Report
      1991 Annual Research Report
  • [Publications] Tadamichi Shimizu: "Immunoreactive analogues of erythrocyte ankyrin in human epidermal keratinocytes" submitted.

    • Related Report
      1991 Annual Research Report
  • [Publications] Hiroko Koizumi: "Immunoblot demonstration of protein kinase C in pig epidermis." Journal of Dermatology. 17. 24-27 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] Tadamichi Shimizu: "Immunohistochemical analysis of human skin using antiーspectrin and antiーβーfodrin antibodies." Archives of Dermatological Research. 282. 274-277 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] Toshiyuki Nakagaki: "Ultraviolet action spectrum for intracellular free Ca2+ increase in human epidermal keratinocytes." Cell Structure and Function. 15. 175-179 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] Hiroko Koizumi: "Translocation of protein kinase C from cytosol to membrane fractions in human epidermal keratinocytes by recombinant interferonーgamma." Jounal of Dermatological Science. 1. 237-310 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] Hiroko Koizumi: "Effect of 1,25ーdihydroxyvitamin D3 on adenylate cyclase and protein kinase C in pig epidermis." Acta Dermatovenereologica (Stockholm). 71. 6-10 (1991)

    • Related Report
      1990 Annual Research Report
  • [Publications] Hiroko Koizumi: "Betaーadrenergic stimulation induces intracellular Ca2+ increase in human epidermal keratinocytes." Journal of Investigative Dermatology. 96. 234-237 (1991)

    • Related Report
      1990 Annual Research Report
  • [Publications] Yoshifumi Osawa: "Adenylate cyclase induces intracellular Ca2+ increase in single human epidermal keratinocytes of the epidermal sheet as measured by digital imaging microscopy using fura 2ーAM" Archives of dermatological Research. 283. (1991)

    • Related Report
      1990 Annual Research Report
  • [Publications] Tadamichi Shimizu: "Presence and localization of proteins immunologically related to erythrocyte protein 4.1 in skin." Histochemistry. (1991)

    • Related Report
      1990 Annual Research Report
  • [Publications] Chikako Yasui: "Adenylate cyclase induces intracellular calcium increase in human epidermal keratinocytes.(abstract)" Journal of dermatological Science(JDSECI). 1. 402 (1990)

    • Related Report
      1990 Annual Research Report

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Published: 1990-04-01   Modified: 2016-04-21  

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