Characterization of a novel anti-ENA antibodies

• Project/Area Number: 02670486
• Research Category: Grant-in-Aid for General Scientific Research (C)
• Allocation Type: Single-year Grants
• Research Field: Dermatology
• Research Institution: Nara Medical University (C)
• Principal Investigator: MIYAGAWA Sachiko Nara Medical University Department of Dermatology Associate Professor, 医学部, 助教授 (30094626)
• Co-Investigator(Kenkyū-buntansha): OKADA Natsuko Osaka University Medical School Department of Dermatology Assistant Professor, 医学部, 講師 (60135697) ; YOSHIOKA Akira Nara Medical University Department of Pediatrics Associate Professor, 医学部, 助教授 (40106498)
• Project Period (FY): 1990 – 1991
• Project Status: Completed (Fiscal Year 1991)
• Budget Amount: ¥2,400,000 (Direct Cost: ¥2,400,000); Fiscal Year 1991: ¥1,200,000 (Direct Cost: ¥1,200,000); Fiscal Year 1990: ¥1,200,000 (Direct Cost: ¥1,200,000)
• Keywords: antinuclear antibodies / extractable nuclear antigens / thrombocytopenic purpura / iopathic throbocyopenic purpura / antinuclear antibodies / antiーENA antibodies

Research Abstract

Circulating antibodies against certain extractable nuclear antigens (ENA) have been shown to have diagnostic importance in connective tissue diseases. We reported an antibody against ENA found in the sera of patients with idiopathic thrombocytopenic purpura (ITP). The antigen, tentatively designated TPNA (thrombocytopenic purpura associated nuclear antigen) was distinct from UlRNP/Sm, SSA/SSB, Scl-70, PCNA, PM-Scl, Jo-1, and Ku by immunodiffusion. On immunoblotting, the anti-TPNA serum recognized polypeptides of 99 kd, 98 kd, and 96 kd in rabbit thymus extracts and a 99 kd polypeptide in KB cell extracts. The anti-TPNA antibody was detected in the sera of 2 out of 360 ANA positive patients, both children with clinical features of ITP. One patient developed systemic lupus erythematosus eight years after the onset of ITP.

Research Products

• [Publications] Miyagawa S,Inagaki Y,Okada N,et al: "A novel anti-ENA antibody in sera of patients with childhood idiopathic thrombocytopenic purpura." J Dermatol. 18. 69-73 (1991)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] 倉田 義之,宮川 幸子,小杉 智他: "ITPにおける抗核抗体の臨床的意義" 日本臨床血液学会雑誌.
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] Miyagawa S, Inagaki Y, Okasa N, et al: "A novel anti-ENA antibody in sera of patients with childhood idiopathic thrombocytopenic purpura." J Dermatol. 18. 69-73 (1991)
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] Kurata Y, Miyagawa S, Kosugi S, et al: "Clinical significance of antinuelear antibodies in ITP" Jap J Clin Hematol.
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] Miyagawa S,Inagaki Y,Okada N,et al: "A novel antiーENA antibody in sera of patients with childhood idiopathic thrombocytopenic purpura." J Dermatol. 18. 69-73 (1991)
• [Publications] 倉田 義之,宮川 幸子,小杉 智 他: "ITPにおける抗核抗体の臨床的意義" 日本臨床血液学会雑誌.
• [Publications] Miyagawa S,Inagaki Y,Okada N,et al: "A novel antiーENA antibody in sera of patients with childhood idiopathic thrombocytopenic purpura." Journal of Dermatology. 18. (1991)