| Project/Area Number |
02670542
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
General surgery
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| Research Institution | KYOTO UNIVERSITY |
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Principal Investigator |
SUZUKI Shigehiko Faculty of Med. Plastic Surgery Assist. Professor, 医学部, 助教授 (30187728)
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| Project Period (FY) |
1990 – 1992
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| Project Status |
Completed (Fiscal Year 1992)
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| Budget Amount *help |
¥2,300,000 (Direct Cost: ¥2,300,000)
Fiscal Year 1992: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 1991: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 1990: ¥1,100,000 (Direct Cost: ¥1,100,000)
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| Keywords | SOD / Reactive oxygen species / Skin flap / Antioxidant / Lipid peroxide / Fat flap / 過酸化脂貿 / 虚血再潅流障害 |
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| Research Abstract |
1)In attempts to substantiate the possible participation of reactive oxygen species, and significance of the xanthine oxidase system in both post-ischemic reperfusion necrosis of the island flap and distal necrosis of the pedicle flap, and to develop new pharmacological measures for salvaging flap necrosis, a series of experiments were made using an island flap model and a random-pattern flap model in rats. The results demonstrated a possible involvement of ROS in both post-ischemic necrosis of island flaps and distal necrosis of random flaps. Xanthine oxidase was significant in producing ROS only in the former.
2)In order to examine the effect of topical use of SOD ointment which contains 1% SOD on the enhancement of flap survival, three experiments were made. Its application to the under surface of the flap or occlusive dressing was effective.
3)Involvement of lipid peroxidation in flap necrosis and effect of topical application of herbal antioxidant in salvagng flap necrosis were investigated. Among the herbal antioxidants, ellagic acid exerted the strongest effect in suppressing lipid peroxide levels and increasing flap survival.
4)Ischemic-reperfusion injury of island fat flap of rats was also investigated.
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