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Experimental study of hypoplastic lungs with surgical disease in fetus.

Research Project

Project/Area Number 02670554
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field General surgery
Research InstitutionKagoshima University

Principal Investigator

TAKAMATSU Hideo (1991-1992)  Kagoshima University, Faculty of medicine, Professor, 医学部, 教授 (50142427)

秋山 洋 (1990)  鹿児島大学, 医学部附属病院, 教授 (60167856)

Co-Investigator(Kenkyū-buntansha) TAHARA Hiroyuki  Kagoshima University, Faculty of medicine, Research associate, 医学部, 助手 (70236719)
NOGUCHI Hiroyuki  Kagoshima University, Faculty of medicine, Assistant professor, 医学部, 講師 (80198580)
高松 英夫  鹿児島大学, 医学部附属病院, 講師 (50142427)
Project Period (FY) 1990 – 1992
Project Status Completed (Fiscal Year 1992)
Budget Amount *help
¥2,600,000 (Direct Cost: ¥2,600,000)
Fiscal Year 1992: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 1991: ¥900,000 (Direct Cost: ¥900,000)
Fiscal Year 1990: ¥900,000 (Direct Cost: ¥900,000)
KeywordsHypoplastic lung / Congenital diaphragmatic hernia / Nitrofen / Tenascin
Research Abstract

We tried to make an experimental animal model for the investigation of fetal hypoplastic lungs with surgical disease.
It has been well known that Nitrofen(2, 4-dichloro-4 nitrodiphenylether) induces in high frequency various types of CDH(congenital diaphragmatic hernia) and hypoplastic lungs in mice and rats.
One hundred mg or two hundred and fifty mg per kg bodyweight of Nitrofen with 3-5ml olive oil was administered to pregnant rat by intragastric intubation on 9th or 11th day of gestation. A dam was sacrificed on the 20th of gestation and fetuses were removed from a dam. The number of animals with diaphragmatic defect, position(right- or left-side), intrathoracic contents were evaluated. And hypoplastic lungs were studied immunohistochemically with Tenascin which is an extracelluar matrix glycoprotein playing a vital role in pathological change (embryogenesis, certain sequential events such as cell death, proliferation, attachment, detachment, migration, morphogenesis and cytodifferentiation).
Administration of 250mg per kg bodyweight of Nitrofen on 9th day of gestation induced diaphragmatic defect most frequently.
Immunohistochemically, expression of Tenascin was not observed in hypoplasic lung nor normal lung.

Report

(4 results)
  • 1992 Annual Research Report   Final Research Report Summary
  • 1991 Annual Research Report
  • 1990 Annual Research Report

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Published: 1990-04-01   Modified: 2016-04-21  

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