| Project/Area Number |
02670568
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
Digestive surgery
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| Research Institution | NIIGATA UNIVERSITY |
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Principal Investigator |
SUZUKI Tsutomu Niigata University School of Medicine University Hospital The First Department of Surgery Lecturer, 医学部附属病院, 講師 (40183420)
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| Co-Investigator(Kenkyū-buntansha) |
SUZUKI Satoshi Niigata University School of Medicine University Hospital The First Department o, 医学部附属病院, 医員
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| Project Period (FY) |
1990 – 1992
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| Project Status |
Completed (Fiscal Year 1992)
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| Budget Amount *help |
¥2,400,000 (Direct Cost: ¥2,400,000)
Fiscal Year 1992: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 1991: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 1990: ¥600,000 (Direct Cost: ¥600,000)
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| Keywords | Cancer Metastsis / Genetic Instability / DNA Recombinational Mutation / DNA Fingerprint / Minisatellite DNA / Patient Prognosis / ケルセチン / DNA操み換え変異 / ミニサテライト DNA / 癌細胞 / DNAヘテロ化 / Pcー1,Pcー2 |
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| Research Abstract |
Mutagenicity of quercetin, a flavonoid, was examined by means of DNA fingerprint analysis using the Pc-1 and Pc-2 minisatellite probes that efficiently detect mutations due to recombination. Treatment of FM3A and BMT-11 tumor cells with 55 muM quercetin resulted in gain and loss of bands in the fingerprints in both cell lines. The frequencies of the clones having undergone mutation were 6/26 and 3/11, using Pc-1 probe, respectively. These results seem to provide a molecular basis for the phenotypic variations of BMT-11 tumor cells induced by quercetin, providing direct evidence of genetic instability of the tumor cells. Moreover, we tested the corelation between frequencies of DNA recombinational mutations and intensiveness of malignancy in human colon cancers. DNA of eight human colon cancer tissue and corresponding peripheral blood cells were prepared, respectively, and examined by DNA fingerprint analysis using Myo and hPc-1 polymorphic minisatellite probes. These eight specimens exhibited no extra-bands resulting from recombination and/or DNA slippage at present. We would explain how the prognosis of cancer patients is related to frequencies of DNA recombinational mutation.
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