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Experimental study on the pathogenesis of acute acalculous cholecysitis, with special reference to the roles of microcirculatory disturbances, free radicals and prostaglandins.

Research Project

Project/Area Number 02670574
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field Digestive surgery
Research InstitutionMie University

Principal Investigator

OGURA Yoshifumi  Mie University School of Medicine, Assistant professor, 医学部, 講師 (00126963)

Project Period (FY) 1990 – 1991
Project Status Completed (Fiscal Year 1991)
Budget Amount *help
¥2,100,000 (Direct Cost: ¥2,100,000)
Fiscal Year 1991: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 1990: ¥1,600,000 (Direct Cost: ¥1,600,000)
Keywordsacute acalculous cholecystitis / ischemia-reperfusion injury / mecrocirculatory disturbance / prostaglandin / cholesterosis / radical scavenger / quinacrine / superoxide dismutase / 虚血・再還流 / フリ-ラジカル / 膜結合性PLA_2 / 過酸化脂質 / SOD
Research Abstract

To elucidate the pathogenesis of acute acalculaous cholecystitis, the gallbladder was subjected to ischemia-reperfusion by simultaneously occlusing the middle hepatic artery and the superior mesenteric vein in dogs, and the degree of inflammation and biochemcal changes in the gallbladder mucosa were studied by yarying the duration of ischemia or reperfusion. Cholecystitis was produced in all animals by 45-min ischemia followed by 90-min reperfusion as the shortest ischemia and reperfusion times. In this model, prologation of the ischemic time increased the area of mucosal inflammation horizontally with in-creases of the PLA_2 activity. content of lipoid peroxide, whereas by prolonging the reperfusion time the inflammation area spread deeper vertically toward the serosal side with significant increase in the mucosal PLA_2 activity, content of lipid peroxide and SOD activity. Moreover, the present study revealed that free radicals were eliminated efficiently by the intraarterial infusion of a mixuture of SOD and CAT, clearly suppressing the development of cholecystitis.
The levels of PGE_2, PGF_2alpha, 6KF, and TXB_2 in the gallbladder lavage fluid increased significantly after ischemia-reperfusion, and especially PGE_2 level was correlated well with inflammation area of the gallbladder mucosa. Infusion of a mixture of SOD, CAT and quinacrine into the cystic artery during the reperfusion time causd the reduction in the levels of PGE_2 and PGF_2 alpha in the gallbladder lavage fluid and inflammation area in the gallbladder.
The repeating ischemia-reperfusion of the gallbladder caused cholesterosis, reducation of pH, a decrease in the concentration of bile acid and lecithin in the gallbladder bile, and an increase in the cholesterol saturation index.

Report

(3 results)
  • 1991 Annual Research Report   Final Research Report Summary
  • 1990 Annual Research Report
  • Research Products

    (17 results)

All Other

All Publications (17 results)

  • [Publications] 田岡 大樹: "胆道疾患とフリ-ラジカル ー特に急性無石胆嚢炎の発生機序と関連してー" 肝胆膵. 16(2). 255-262 (1988)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] 小倉 嘉文: "気腫性胆嚢炎" 肝胆膵. 18(1). 51-59 (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] 田岡 大樹: "胆嚢疾患とフリ-ラジカル ー急性無石胆嚢炎の臨床的特徴と実験的検討ー" 活性酸素・フリ-ラジカル. 2(5). 630-637 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] 金田 真: "胆嚢コレステロ-シスの発生機序に関する実験的研究" 胆道. 5(5). 499-512 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Hiroki Taoka: "Experimental study on the pathogenesis of acute acalculous cholecystitis,with special reference to the roles of microcirculatory disturbances, free radicals and membrane-bound phospholipase Λ_2" Gastroenterologia Japonica. 26(5). 633-644 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Hiroki Taoka: "The role of free radicals in pathophysiology of bile duct disease -especially on the pathogenesis of acute acalculous cholecystitis-" KAN・TAN・SUI (Japan). 16(2). 225-262 (1988)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Yoshifumi Ogura: "Emphysematous cholecysitits" KAN・TAN・SUI (Japan). 18(1). 51-59 (1989)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Hiroki Taoka: "Acute acalculous cholecystitis and free radical" Journal of Active Oxygens & Free Radicals. 2(5). 630-637 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Makoto Kaneda: "Experimental stuey on pathogenesis of cholesterosis of the gallbladder" Journal of Japan Biliary Association. 5(5). 499-512 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Hiroki Taoka: "Experimental study on the pathogenesis of acute acalculous cholecystitis, with special reference to the roles of microcirculatory disturbances, free radicals and membrane-bound phospholipase A_2." Gastroenterologia Japonica. 26(5). 633-644 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] 田岡 大樹: "胆道疾患とフリ-ラジカルー特に急性無石胆嚢炎の発生機序と関連してー" 肝胆膵. 16(2). 255-262 (1988)

    • Related Report
      1991 Annual Research Report
  • [Publications] 小倉 嘉文: "気腫性胆嚢炎" 肝胆膵. 18(1). 51-59 (1989)

    • Related Report
      1991 Annual Research Report
  • [Publications] 田岡 大樹: "胆嚢疾患とフリ-ラジカルー急性無石胆嚢炎の臨床的特徴と実験的検討ー" 活性酸素・フリ-ラジカル. 2(5). 630-637 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] 金田 真: "胆嚢コレステロ-シスの発生機序に関する実験的研究" 胆道. 5(5). 499-512 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] Hiroki Taoka: "Experimental study on the pathogenesis of acute acalculous cholecystitis,with special reference to the roles of microcirculatory disturbances,free radicals and membraneーbound phospholipase ∧_2" Gastroenterologia Japonica. 26(5). 633-644 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] 田岡 大樹: "胆道疾患とフリ-ラジカルー特に急性無石胆嚢炎の発生機序と関連して" 肝胆膵. 16. 255-262 (1988)

    • Related Report
      1990 Annual Research Report
  • [Publications] 小倉 嘉文: "気腫性胆嚢炎" 肝胆膵. 18. 51-59 (1989)

    • Related Report
      1990 Annual Research Report

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Published: 1990-04-01   Modified: 2016-04-21  

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